Controlled carbon nanotube layers for impedimetric immunosensors: High performance label free detection and quantification of anti-cholera toxin antibody

Palomar, Quentin; Gondran, Chantal; Holzinger, Michael; Marks, Robert S.; Cosnier, Serge

doi:10.1016/j.bios.2017.05.052

Cited by 39 publications

(25 citation statements)

References 39 publications

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“…Label-free and single-immunoreaction biosensors have been reported using electrode substrates nanostructured with PPI-AuNP nanocomposite [ 81 ], PANnf’s [ 82 ], ZnO NPs [ 83 ], CNTs [ 84 ] and CNFs [ 85 ] for Vibrio cholerae [ 82 ] and its characteristic toxins [ 81 , 82 , 85 ] or antibodies [ 84 ]. Although all these immunosensors exhibit good analytical characteristics in terms of sensitivity and selectivity, none has been applied to the analysis of real samples.…”

Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

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Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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The excellent capabilities demonstrated over the last few years by electrochemical affinity biosensors should be largely attributed to their coupling with particular nanostructures including dendrimers, DNA-based nanoskeletons, molecular imprinted polymers, metal-organic frameworks, nanozymes and magnetic and mesoporous silica nanoparticles. This review article aims to give, by highlighting representative methods reported in the last 5 years, an updated and general overview of the main improvements that the use of such well-ordered nanomaterials as electrode modifiers or advanced labels confer to electrochemical affinity biosensors in terms of sensitivity, selectivity, stability, conductivity and biocompatibility focused on food and environmental applications, less covered in the literature than clinics. A wide variety of bioreceptors (antibodies, DNAs, aptamers, lectins, mast cells, DNAzymes), affinity reactions (single, sandwich, competitive and displacement) and detection strategies (label-free or label-based using mainly natural but also artificial enzymes), whose performance is substantially improved when used in conjunction with nanostructured systems, are critically discussed together with the great diversity of molecular targets that nanostructured affinity biosensors are able to quantify using quite simple protocols in a wide variety of matrices and with the sensitivity required by legislation. The large number of possibilities and the versatility of these approaches, the main challenges to face in order to achieve other pursued capabilities (development of antifouling, continuous operation, wash-, calibration- and reagents-free devices, regulatory or Association of Official Analytical Chemists, AOAC, approval) and decisive future actions to achieve the commercialization and acceptance of these devices in our daily routine are also noted at the end.

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Section: Selected Nanostructures In Electrochemical Biosensing Formentioning

confidence: 99%

“… Scheme of an EIS-based immunosensor for anti-cholera toxin antibodies prepared by coordinative binding of the biotinylated cholera toxin on poly(pyrrole-NTA)/Cu 2+ electropolymerized at a MWCNTs/GCE, and Nyquist plots obtained by measurement of [Fe(CN) 6 ] 4−/3− . Reprinted from [ 84 ] with permission. …”

Section: Figures Scheme and Tablesmentioning

confidence: 99%

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Campuzano

Yáñez‐Sedeño

Pingarrón

2020

Sensors

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“…Because of the WHO’s goal to increase the global capacity to monitor and control the major epidemics and pandemic threats, sensitive, rapid, and predictable detection of specific antibodies by biosensors has become particularly important for clinical serodiagnosis [ 3 , 5 , 6 , 55 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 ].…”

Section: Potential Applications Of Antibody Biosensing For Clinicamentioning

confidence: 99%

“…Using the specific antigen as an MRE and CNTs to enhance the electric properties, electrical changes could be measured at antibody concentration as low as 6~50 pg L −1 , and as high as ~70 μg L −1 . Recently, another EIS-based immunosensor was also developed based on CNTs deposits [ 83 ]. The bioreceptor unit, biotin-modified cholera toxin B subunit, was immobilized with the nitrilotriacetic acid-Cu(II) complex.…”

Section: Potential Applications Of Antibody Biosensing For Clinicamentioning

confidence: 99%

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Jiang

2017

Sensors

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Biosensors for proteins have shown attractive advantages compared to traditional techniques in clinical laboratory diagnosis. In virtue of modern fabrication modes and detection techniques, various immunosensing platforms have been reported on basis of the specific recognition between antigen-antibody pairs. In addition to profit from the development of nanotechnology and molecular biology, diverse fabrication and signal amplification strategies have been designed for detection of protein antigens, which has led to great achievements in fast quantitative and simultaneous testing with extremely high sensitivity and specificity. Besides antigens, determination of antibodies also possesses great significance for clinical laboratory diagnosis. In this review, we will categorize recent immunosensors for proteins by different detection techniques. The basic conception of detection techniques, sensing mechanisms, and the relevant signal amplification strategies are introduced. Since antibodies and antigens have an equal position to each other in immunosensing, all biosensing strategies for antigens can be extended to antibodies under appropriate optimizations. Biosensors for antibodies are summarized, focusing on potential applications in clinical laboratory diagnosis, such as a series of biomarkers for infectious diseases and autoimmune diseases, and an evaluation of vaccine immunity. The excellent performances of these biosensors provide a prospective space for future antibody-detection-based disease serodiagnosis.

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“…Solution processing of CNT-based films, therefore, offers great potential for the cost-effective preparation of devices such as flexible electrodes, supercapacitors, biosensors, and biofuel cells [ 23 , 24 , 25 , 26 ]. For the construction of bioelectrodes, the simple drop-casting of CNTs from aqueous and non-aqueous suspensions represents the most commonly employed method [ 7 , 27 ].…”

Section: Introductionmentioning

confidence: 99%

Flotation Assembly of Large-Area Ultrathin MWCNT Nanofilms for Construction of Bioelectrodes

et al. 2017

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Abstract:We report a simple, versatile, and rapid method for the fabrication of optically-transparent large-area carbon nanotube (CNT) films via flotation assembly. After solvent-induced assembly, floating films were transferred to a flat supporting substrate to form conductive and transparent CNT film electrodes. The resulting electrodes, with uniform 40 ± 20 nm multi-walled CNT (MWCNT) layers, were characterized by electrochemical and microscopy methods. The flotation method does not require specialized thin-film instrumentation and avoids the need for surfactants and pre-oxidized CNTs which can hamper electrochemical performance. A proof-of-concept nanostructured bioelectrode demonstrating high sensitivity for glucose was developed with an electropolymerized poly(pyrene-adamantane) layer for host-guest immobilization of active β-cyclodextrin tagged GOx enzymes. The polymer provides pyrene groups for cross-linking to CNTs and pendant adamantane groups for binding the β-cyclodextrin groups of the tagged enzyme. This demonstration offers a new approach for the preparation of stable and transparent CNT film electrodes with attractive electrochemical properties towards future photobio-and bio-electrochemical fuel cells, electrochemical sensors, and electroanalysis.

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Controlled carbon nanotube layers for impedimetric immunosensors: High performance label free detection and quantification of anti-cholera toxin antibody

Cited by 39 publications

References 39 publications

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Electrochemical Affinity Biosensors Based on Selected Nanostructures for Food and Environmental Monitoring

Construction and Potential Applications of Biosensors for Proteins in Clinical Laboratory Diagnosis

Flotation Assembly of Large-Area Ultrathin MWCNT Nanofilms for Construction of Bioelectrodes

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