Controlled immobilization-traction based on intervertebral stability is conducive to the regeneration or repair of the degenerative disc: an in vivo study on the rat coccygeal model

Che, Yanjun; Guo, Jiangbo; Liang, Ting; Chen, Xi; Zhang, Wen; Yang, Huilin

doi:10.1016/j.spinee.2018.10.018

Cited by 13 publications

(17 citation statements)

References 43 publications

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“…A reliable method to generate IVD defects is essential in order to investigate pathophysiology and therapeutic interventions for IVD degeneration. Different strategies have been used to induce degenerative changes in rats, including the naturally occurring disc degeneration in aged animals, 18‐21 surgery injury, 4,5,7,12,13,22‐27 and mechanical loading 28‐40 . The process of naturally induced IVD degeneration is slow and the severity of tissue damage can vary between animals.…”

Section: Introductionmentioning

confidence: 99%

“…Different strategies have been used to induce degenerative changes in rats, including the naturally occurring disc degeneration in aged animals, [18][19][20][21] surgery injury, 4,5,7,12,13,[22][23][24][25][26][27] and mechanical loading. [28][29][30][31][32][33][34][35][36][37][38][39][40] The process of naturally induced IVD degeneration is slow and the severity of tissue damage can vary between animals. Surgical injury is widely adopted for inducing degeneration in lumbar IVD, while both surgical and mechanical approaches are commonly used for coccygeal IVDs.…”

Section: Introductionmentioning

confidence: 99%

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Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section

et al. 2021

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Background: Rats are a widely accepted preclinical model for evaluating intervertebral disc (IVD) degeneration and regeneration. IVD morphology is commonly assessed using histology, which forms the foundation for quantifying the state of IVD degeneration. IVD degeneration severity is evaluated using different grading systems that focus on distinct degenerative features. A standard grading system would facilitate more accurate comparison across laboratories and more robust comparisons of different models and interventions.Aims: This study aimed to develop a histology grading system to quantify IVD degeneration for different rat models.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section

et al. 2021

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“…The caudal discs were evaluated and analyzed by X-ray and MRI imaging after each time point [ 7 , 12 , 13 ]. Intervertebral disc space was measured using the Masuda method [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

Regenerating and repairing degenerative intervertebral discs by regulating the micro/nano environment of degenerative bony endplates based on low-tension mechanics

Jing

Guo

Hao

et al. 2022

BMC Musculoskelet Disord

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Background Conservative treatment is the recommended first-line treatment for degenerative disc diseases. Traction therapy has historically been one of the most common clinical methods to address this, but the clinical effect remains controversial. Methods Forty-two six-month-old male Sprague-Dawley rats were randomly divided into six groups: the model group (Group A, four coccyx vertebrae (Co7-Co10) were fixed with customized external fixators, and the vertebral disc degeneration model was constructed by axial compression of the target segment Co8 - Co9 for 4 weeks), the experimental control group (Group B, after successful modeling, the external fixation device was removed and self-rehabilitation was performed) and four intervention groups (Groups C to F): Groups C and E: Co8 - Co9 vertebrae compressed for 4 weeks followed by two or 4 weeks of high tension traction (HTT), respectively, and Groups D and F: vertebrae compressed for 4 weeks followed by two or 4 weeks of low-tension traction (LTT), respectively. Imaging tests (X-ray and MRI) were performed to assess disc height and T2 signal intensity at each time point. After the experiment, the animals were euthanized, and the caudal vertebrae were collected for analysis of intervertebral disc histopathology, proteoglycan content, and micronanostructure of the annulus fibrosus, nucleus pulposus and bony endplate. Results Signs of tissue regeneration were apparent in all four intervention groups. After two to 4 weeks of intervention (HTT and LTT), the morphology of pores in the bony endplate, their number, and diameter had recovered significantly compared with those in Group A. The LTT group was superior to the HTT group, and the 4w in situ group was significantly superior to the 2w group. Meanwhile, the histological scores of discs, the mean fibril diameter and modulus of annulus fibrosus were significantly improved compared with the control groups, and the LTT group was superior to HTT group. Conclusions Low-tension traction better promotes active reconstruction of bony endplates and improves the elastic modulus and micro/nanostructure of the disc. Thus, it further promotes the regeneration and repair of intervertebral discs.

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“…The muscles and surrounding ligaments were carefully cut from the specimens after the mice were killed. The intact tibial plateau and a part of the tibial shaft were placed in a tube containing a mixed enzyme solution of type I and type II collagenase (4%) and then digested in a 37 • C incubator for 1 week [22]. Following digestion of the articular cartilage of the tibial plateau, the subchondral bone of each sample was exposed.…”

Section: Visual Observation By Semmentioning

confidence: 99%

Microstructure and mechanical properties of subchondral bone are negatively regulated by tramadol in osteoarthritis in mice

Liu

Shao

et al. 2020

Bioscience Reports

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Objectives: In the treatment of osteoarthritis (OA), tramadol, a common weak opioid, has become popular due to its effectiveness in inhibition of pain. In this study, we aimed to explore the effect of tramadol on subchondral bone, especially changes in the microstructure and mechanical properties. Methods: A mouse model of OA was established in the present study by destabilization of the medial meniscus (DMM). A vehicle or drug was administered for 4 weeks. Specimens were harvested and analyzed radiologically and histologically using micro-CT, scanning electron microscopy (SEM), atomic force microscopy (AFM) and histological staining to evaluate the knee joints of mice undergoing different forms of intervention. Results: In the early stages of OA induced by DMM, the subchondral bone volume fraction in the OA group was significantly higher than in the sham+vehicle group, while the volume in the treatment groups was lower than in the DMM+vehicle and sham+vehicle groups. In addition, the elastic moduli in the treatment groups clearly decreased compared with the DMM+vehicle and sham+vehicle groups. Observations of the subchondral bone surface by SEM indicated serious destruction, principally manifesting as a decrease in lacunae and more numerous and scattered cracks. Histological staining demonstrated that there was no difference in the degeneration of either the articular cartilage or synovial cells whether tramadol was used or not. Conclusion: Although tramadol is effective in inhibiting pain in early OA, it negatively regulates the microstructure and mechanical properties of subchondral bone in joints.

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Controlled immobilization-traction based on intervertebral stability is conducive to the regeneration or repair of the degenerative disc: an in vivo study on the rat coccygeal model

Cited by 13 publications

References 43 publications

Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section

Development of a standardized histopathology scoring system for intervertebral disc degeneration in rat models: An initiative of the ORS spine section

Regenerating and repairing degenerative intervertebral discs by regulating the micro/nano environment of degenerative bony endplates based on low-tension mechanics

Microstructure and mechanical properties of subchondral bone are negatively regulated by tramadol in osteoarthritis in mice

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