Controlled partial skin thickness burns: an animal model for studies of burnwound progression

Knabl, Joerg.S; Bayer, G. S.; Bauer, W.; Schwendenwein, Ilse; Dado, Petra F; Kucher, Christian; Horvat, Reinhart; Turkof, Edvin; Schossmann, Bernd; Meissl, G.

doi:10.1016/s0305-4179(98)00172-7

Cited by 42 publications

(27 citation statements)

References 28 publications

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“…Copper thermal conductivity is more efficient than other metals used by researchers 1,7,12 . The results clearly demonstrated that this experimental model provided excellent contrast between normal and burned tissue at different temperatures.…”

Section: Discussionmentioning

confidence: 99%

An optimized animal model for partial and total skin thickness burns studies

Campelo¹,

Campelo²,

Britto³

et al. 2011

Acta Cir. Bras.

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PURPOSE: Development of an improved animal model for studying skin burns in rats. METHODS: Twenty-four male Wistar rats were randomly assigned to four groups (n=6): G1-Control, G2-T100°C, G3-T150°C and G4-T200°C. Two 10 x 10 mm squares were outlined with a sterile surgical marker on each side and along the vertebral column using a prepared template positioned between the anterior and posterior limbs. G2-G4 rats were subjected to 100°C, 150°C and 200ºC thermal burns, respectively. G1 rats served as controls. Burns were inflicted by applying a copper plate connected to an electronic temperature controlling device to the dorsal skin of anesthetized rats. Four burns were produced on each animal (total area: 4 cm 2 / animal) leaving about 1 cm of undamaged skin between burn areas. Analgesia was administered during 24 h after burn injury by adding 30 mg codeine phosphate hemihydrate to 500 ml tap water. RESULTS:The application of 100°C and 150ºC resulted in partial thickness skin burns with central reepithelialization of the burned area only at 100°C. In G4 group the whole thickness of the skin was injured without central reepithelialization. However, there was marginal reepithelialization in all groups. CONCLUSION: The model studied is inexpensive and easily reproducible, enabling the achievement of controlled burns with partial or total impairment of the skin in experimental animals. Keywords: Models, Animal. Hot Temperature. Burns. Rats. RESUMO OBJETIVO: Desenvolvimento de um modelo animal aperfeiçoado para estudo de queimaduras cutâneas em ratos. MÉTODOS: Vinte e quatro ratos Wistar, machos, foram distribuídos aleatoriamente em quatro grupos (n=6): G1-Controle, G2-T100°C, G3-T150°C e G4-T200°C. Dois quadrados medindo 10x10 mm foram delineados com um marcador cirúrgico estéril em cada lado e ao longo da coluna vertebral e posicionados entre os membros anteriores e posteriores, utilizando um molde previamente preparado. Os ratos dos grupos G2-G4 foram submetidos a queimaduras térmicas de 100°C, 150°C e 200°C, respectivamente. O grupo G1 foi utilizado como controle. As queimaduras foram infligidas pela aplicação de uma placa de cobre, ligada a um dispositivo de controle eletrônico de temperatura, na pele dorsal de ratos anestesiados. Quatro queimaduras foram produzidas em cada animal (área total: 4 cm2/animal), deixando cerca de 1 cm de pele intacta entre as áreas queimadas. Analgesia foi obtida durante 24 horas após a queimadura por adição de 30mg de fosfato hemi-hidratado de codeína a 500 ml de água potável. RESULTADOS: A aplicação 100°C e 150°C resultou na produção de queimaduras profundas comprometendo parte da espessura da pele, com reepitelização central da área queimada, somente a 100°C. No grupo G4 houve lesão de toda a espessura da pele sem reepitelização central. Entretanto, observou-se reepitelização marginal em todos os grupos estudados. CONCLUSÃO: O modelo estudado é de baixo custo e facilmente reproduzível, propiciando a obtenção controlada de queimaduras com comprometimento parcial ou total da...

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Section: Discussionmentioning

confidence: 99%

An optimized animal model for partial and total skin thickness burns studies

Campelo¹,

Campelo²,

Britto³

et al. 2011

Acta Cir. Bras.

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show abstract

“…Initially, it is important to explain that in the model we designed we took into consideration a couple of important facts: depilation is mandatory because hair may hinder the achievement of a uniform burn [7,8] and the demarcation of necrosis was stable by the third day [5,7], this being the reason why the animals were killed by that time. Burns can be classified into first, second and third degrees, with first-degree burns being limited to the epidermis, seconddegree burns involving the epidermis and part of the dermis, and third-degree burns destroying the epidermis and all dermis [6,16].…”

Section: Discussionmentioning

confidence: 99%

“…In contact burn models, it is imperative to have a large enough area of skin to permit a full contact [5,7] with uniform pressure [19] since pressure has a direct influence on burn depth. In the present model, these issues were addressed by positioning the animal on its side, with fixation of the excess of skin located on the lateral wall of the thorax to a solid base (Styrofoam) associated with the addition of weight to the apparatus (1 kg) to allow a uniform pressure contact.…”

Section: Discussionmentioning

confidence: 99%

“…In order to evaluate the effectiveness of burn wound treatment, however, it is extremely important to be able to create predictable and uniform burns, where most of the confounding variables can be eliminated. Burn depth, defined by three different elements -temperature, time of exposure, and contact pressure -is the primary determinant of prognosis [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A new model for the standardization of experimental burn wounds

Venter

Monte‐Alto‐Costa

Marques

2015

Burns

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“…[12][13][14][15][16][17][18][19][20][21][22] When defining present model, rats were selected because they are easily acquired, inexpensive, and easy to manipulate compared to larger animals. Due to need for only conventional infrared heater and rats, model offers genuinely low cost alternative for burn studies.…”

Section: Discussionmentioning

confidence: 99%