Controlled release and characterisation of photocaged molecules using in situ LED illumination in solution NMR spectroscopy

Abstract

Photocaging is an attractive strategy to control molecular behaviour, for example, in chemical synthesis, interaction studies or photodynamic therapies. Here, we demonstrate that in situ illumination by the LED NMRtorch...

Assessing Photostability of mAb Formulations In Situ Using Light-Coupled NMR Spectroscopy

“…UV-A illumination was performed using an NMRtorch lighthead with an array of 4 × 365 nm LEDs (LZ4-V4UV0R, LED Engin, nominal power 10 W), same as in previous studies, , with illumination controlled by the NMR console and transistor–transistor logic triggers. Samples were placed in 5 mm quartz NMRtorch tubes, prepared as previously described, , and sealed with transparent caps made from hand-polished 7 mm long Suprasil rods (Heraeus) and pieces of fluorinated ethylene propylene (FEP) tubing with 4.5 mm inner diameter.…”

Quality Data from Messy Spectra: How Isometric Points Increase Information Content in Highly Overlapping Spectra

“…The data in b were taken from our recent publication about phosphate detection with the PUB module [9,10] . The data in the remaining panels were originally published by Hutchinson, Welsh and Burés ( c ), [11,12] Lei and colleagues ( d ), [13,14] and Bramham, Zalar, and Golovanov ( e ), [15,16] and I am grateful to the authors for generously providing the raw data upon request. Please see their original publications for the structure of the organocatalyst and the R, R′, and Ar residues.…”

“…For instance, the enzymatic hydrolysis of adenosine triphosphate ( 4 ), yielding the corresponding diphosphate 4* , gives 1 H NMR spectra exhibiting a pseudo‐isointensic point at around 8.37 ppm (Figure 1e). [15] …”

Simple and effective in situ sample illumination for electron paramagnetic resonance

“…The light around the area containing the sample is however scattered, illuminating the sample from the outside. 23,24 This arrangement benefits from the matching cross-section area of the tube end and light emitting surface, minimising the transfer losses and avoiding intermediary optical components such as optical fibres, thus maximising the intensity of light delivered to the sample. 23,24 In early versions of the NMRtorch 23,24 the light scattering centres were introduced via abrasive etching on the outside surface of the glass.…”