Controlled release microparticles as a single dose hepatitis B vaccine: evaluation of immunogenicity in mice

Enhancement of immune response of HBsAg loaded poly (L-lactic acid) microspheres against Hepatitis B through incorporation of alum and chitosan

“…biodegradability and biocompatibility, and has proven safety and a non-toxic profile (Gilding and Reed 1979). Encapsulation studies using PLA for formulating microspheres for vaccine delivery have included several antigens including HBsAg (Singh et al 1997) and PLA microspheres have been found to provide adjuvant activity in inducing immune responses (Raghuvanshi et al 2002). Antigens such as tetanus toxoid (TT), Yersinia pestis V antigen and HBsAg have been encapsulated previously in PLA microspheres (Gilding and Reed 1979;Nellore et al 1992;Almeida et al 1993;Raghuvanshi et al 2002;Eyles et al 2003) and these have provided encouraging results.…”

Antibody Response to Polyhistidine-Tagged Peptide and Protein Antigens Attached to Liposomes via Lipid-Linked Nitrilotriacetic Acid in Mice

“…Precipitation or adsorption onto aluminum salts is the traditional approach, and alum remains the only vaccine adjuvant approved for use in the United States (16,18). Alternatively, proteins can be associated with lipidic or polymeric particulates via encapsulation or chemical conjugation (5,8,28,43). However, these strategies present significant challenges.…”

CpG Oligodeoxynucleotides Adsorbed onto Polylactide-Co-Glycolide Microparticles Improve the Immunogenicity and Protective Activity of the Licensed Anthrax Vaccine

“…Biodegradable cationic polylactide-co-glycolide (PLG) microparticles represent a different form of immune adjuvant. PLG improve the uptake and processing of adsorbed antigen by antigen-presenting cells (3,6,7,28,35,36,37). The current work examines whether CpG ODN adsorbed onto PLG microparticles (CpG ODN-PLG) increase the speed and magnitude of protective anti-PA immunity induced by coadministered AVA.…”