Controlled release of DNA from poly(vinylpyrrolidone) capsules using cleavable linkers

Ng, Sher Leen; Such, Georgina K.; Johnston, Angus P. R.; Antequera-García, Gema; Caruso, Frank

doi:10.1016/j.biomaterials.2011.05.011

Cited by 46 publications

(42 citation statements)

References 38 publications

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“…35,37 Other approaches have relied on the post-fabrication treatment of hydrogen-bonded multilayers with short disulfide-containing linkers to introduce stabilizing crosslinkes that can be cleaved by reducing agents to promote film erosion and release incorporated agents. 38–41 …”

Section: Resultsmentioning

confidence: 99%

Layer-by-Layer Assembly of Amine-Reactive Multilayers Using an Azlactone-Functionalized Polymer and Small-Molecule Diamine Linkers

2017

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We report the reactive layer-by-layer assembly of amine-reactive polymer multilayers using an azlactone-functionalized polymer and small-molecule diamine linkers. This approach yields crosslinked polymer/linker-type films that can be further functionalized, after fabrication, by treatment with functional primary amines, and provides opportunities to incorporate other useful functionality that can be difficult to introduce using other polyamine building blocks. Films fabricated using poly(2-vinyl-4,4-dimethylazlactone) (PVDMA) and three model non-degradable aliphatic diamine linkers yielded reactive thin films that were stable upon incubation in physiologically relevant media. In contrast, films fabricated using PVDMA and varying amounts of the model disulfide-containing diamine linker cystamine were stable in normal physiological media, but were unstable and eroded rapidly upon exposure to chemical reducing agents. We demonstrate that this approach can be used to fabricate functionalized polymer microcapsules that degrade in reducing environments, and that rates of erosion, extents of capsule swelling, and capsule degradation can be tuned by control over the relative concentration of cystamine linker used during fabrication. The polymer/linker approach used here expands the range of properties and functions that can be designed into reactive PVDMA-based coatings, including functionality that can degrade, erode, and undergo triggered destruction in aqueous environments. We therefore anticipate that these approaches will be useful for the functionalization, patterning, and customization of coatings, membranes, capsules, and interfaces of potential utility in biotechnical or biomedical contexts and other areas where degradation and transience are desired. The proof of concept strategies reported here are likely to be general, and should prove useful for the design of amine-reactive coatings containing other functional structures by judicious control of the structures of the linkers used during assembly.

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Section: Resultsmentioning

confidence: 99%

Layer-by-Layer Assembly of Amine-Reactive Multilayers Using an Azlactone-Functionalized Polymer and Small-Molecule Diamine Linkers

2017

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“…Low-fouling PVP capsules assembled via hydrogen bonding and stabilized using covalent cross-linking were investigated as a potential carrier for the development of drug delivery [225] . Ng et al report that the capsules present relative stability and good cargo (fluorescently labelled plasmid DNA) retention over 48 h, which make these capsules very attractive for applications in drug and gene delivery [225] .…”

Section: Challenges Of Pvp For Applications In Pharmaceutical Industrmentioning

confidence: 99%

Poly(vinylpyrrolidone) – A Versatile Polymer for Biomedical and Beyond Medical Applications

Teodorescu¹,

Bercea²

2015

Polymer-Plastics Technology and Engineering

383

222

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The high versatility of poly(vinylpyrrolidone) (PVP) can be explained by its diverse properties including its solubility in water and in a broad range of liquid media, high chemical and thermal resistance, and unique wetting, binding, and film-forming properties. Thanks to biocompatibility, absence of toxicity and high capacity to form interpolymer complexes, PVP is widely used for designing materials for different applications, such as biomaterials for medical and nonmedical uses. This review summarizes a vast diversity of applicative examples showing the tremendous opportunities for future research and developments of PVP based biomaterials.

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“…A major application for PVP is in micellar systems, [152][153][154][155][156][157] although PVP has also been used in liposomes, 158-160 nanostructured capsules, [161][162][163][164] and drug conjugates. A major application for PVP is in micellar systems, [152][153][154][155][156][157] although PVP has also been used in liposomes, 158-160 nanostructured capsules, [161][162][163][164] and drug conjugates.…”

Section: Poly(vinylpyrrolidone)mentioning

confidence: 99%

Antibiofouling polymer interfaces: poly(ethylene glycol) and other promising candidates

2015

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Nonspecific protein adsorption and/or microbial adsorption on biomedical materials adversely affects the efficacy of a range of biomedical systems, from implants and biosensors to nanoparticles. To address this problem, antibiofouling polymers can be coated on biomedical devices or built into nanoparticles to confer protein and/or microbial repellent properties. The current review provides an overview of the range of synthetic polymers currently used to this end and explores their biomedical potential. The most widely-used antifouling polymer, poly(ethylene glycol) (PEG) is reviewed alongside several promising alternatives, including zwitterionic polymers, poly(hydroxyfunctional acrylates), poly(2-oxazoline)s, poly (vinylpyrrolidone), poly(glycerol), peptides and peptoids. For each material, notable applications for both nanomedicine and macroscopic surface coatings are highlighted.

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Controlled release of DNA from poly(vinylpyrrolidone) capsules using cleavable linkers

Cited by 46 publications

References 38 publications

Layer-by-Layer Assembly of Amine-Reactive Multilayers Using an Azlactone-Functionalized Polymer and Small-Molecule Diamine Linkers

Layer-by-Layer Assembly of Amine-Reactive Multilayers Using an Azlactone-Functionalized Polymer and Small-Molecule Diamine Linkers

Poly(vinylpyrrolidone) – A Versatile Polymer for Biomedical and Beyond Medical Applications

Antibiofouling polymer interfaces: poly(ethylene glycol) and other promising candidates

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