Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

Anderson, Alexa; Segura, Tatiana

doi:10.3791/64554

Cited by 6 publications

(7 citation statements)

References 23 publications

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“…The clear associations between cellular morphology and cell phenotype aligned with the profiles in M1/M2 activation shown by previous publication, where macrophages with more circular shape presented a higher expression of M2‐associated markers (CD206 and Arg1+). [ 23 ] This observation led us to suggest that differences in the association of microgel size with macrophages phenotype between this study and Lowen et al.’s work may be due to difference in packing on the microgels [ 38 ] and cell size. Taken together, our results show that that the spatial confinement of MAP scaffolds modulates macrophages phenotype with M1(LPS/IFN γ ) activation.…”

Section: Resultssupporting

confidence: 45%

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds

Liu

Suarez‐Arnedo

Riley

et al. 2023

Adv Healthcare Materials

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Macrophages are essential in the initiation, maintenance, and transition of inflammatory processes such as foreign body response and wound healing. Mounting evidence suggests that physical factors also modulate macrophage activation. 2D in vitro systems demonstrate that constraining macrophages to small areas or channels modulates their phenotypes and changes their responses to known inflammatory agents such as lipopolysaccharide. However, how dimensionality and pore size affect macrophage phenotype is less explored. In this work, the change in macrophage M1/M2 polarization when confined in microporous annealed particle (MAP) scaffolds is studied. Particles sizes (40, 70, and 130 µm) are selected using outputs from software LOVAMAP that analyzes the characteristics of 3D pores in MAP gels. As the size of building block particle correlates with pore size inside the scaffolds, the three types of scaffold allow us to study how the degree of spatial confinement modulates the behavior of embedded macrophages. Spatially confining macrophages in scaffolds with pore size on the scale of cells leads to a reduced level of the inflammatory response, which is correlated with a change in cell morphology and motility.

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Section: Resultssupporting

confidence: 45%

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds

Liu

Suarez‐Arnedo

Riley

et al. 2023

Adv Healthcare Materials

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“…Alexa Fluor 488 C5-tetrazine (Alexa488-Tet) and Alexa Fluor 555 C2-tetrazine (Alexa555-Tet) were synthesized through two base-catalyzed thiol-Michael addition reactions in series, as described previously. [30, 31] Heparin sodium salt (14,000 Da; EMD Millipore) was modified with norbornene functional handles and fabricated into nanoparticles as described previously by Wilson, et al [21] 1 H NMR shifts of pendant norbornene groups at δ6.33 and δ6.02 (vinyl protons, endo) and δ6.26 and δ6.23 ppm (vinyl protons, exo) were compared to the heparin methyl group at δ2.00 ppm to determine functionalization.…”

Section: Experimental Methodsmentioning

confidence: 99%

Engineering the microstructure and spatial bioactivity of MAP scaffolds in vitro instructs neovascularization in vivo

Anderson,

Caston,

Riley

et al. 2023

Preprint

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In tissues where the vasculature is either lacking or abnormal, biomaterials can be designed to promote vessel formation and enhance tissue repair. In this work, we independently tune the microstructure and bioactivity of microporous annealed particle (MAP) scaffolds to guide cell patterning in 3D and promote de novo assembly of endothelial progenitor-like cells into vessels. We implement bothin silicocharacterization andin vitroexperimentation to elucidate an optimal scaffold formulation for vessel formation. We determine that MAP scaffolds with pore volumes on the same order of magnitude as cells facilitate cell growth and vacuole formation. We achieve spatial control over cell spreading by incorporating adhesive microgels in well-mixed, heterogeneous MAP scaffolds. While we demonstrate that integrin engagement is the primary driver of network formation in these materials, introducing adhesive microgels loaded with heparin nanoparticles leads to the formation of vascular tubes after 3 days in culture. We then showin vivothat this unique scaffold formulation enhances vessel maturation in a wound healing model and instructs differential vascular patterning in the tumor microenvironment. Taken together, this work determines the optimal microstructure and ligand presentation within MAP scaffolds that lead to vascular constructsin vitroand facilitate neovascularizationin vivo.

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“…[24] Compared to spherical microgels, the interlinking of rod-shaped microgels leads to the formation of larger pores with less microgel volume. [25] The use of thinner and higher-aspect-ratio microgels produced by in-mold polymerization showed that pore size increases proportionately with the aspect ratio (up to 20), producing scaffolds with porosities of up to 90% and a mean pore size of up to 82 μm. The constructs supported rapid, extensive filling by fibroblasts within one week.…”

Section: Technological Approaches For the Development Of Biohybrid Im...mentioning

confidence: 99%

Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

Fernández‐Colino

Kießling

Slabu

et al. 2023

Adv Healthcare Materials

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Today's living world is enriched with a myriad of natural biological designs, shaped by billions of years of evolution. Unraveling the construction rules of living organisms offers the potential to create new materials and systems for biomedicine. From the close examination of living organisms, several concepts emerge: hierarchy, pattern repetition, adaptation, and irreducible complexity. All these aspects must be tackled to develop transformative materials with lifelike behavior. This perspective article highlights recent progress in the development of transformative biohybrid systems for applications in the fields of tissue regeneration and biomedicine. Advances in computational simulations and data‐driven predictions are also discussed. These tools enable the virtual high‐throughput screening of implant design and performance before committing to fabrication, thus reducing the development time and cost of biomimetic and biohybrid constructs. The ongoing progress of imaging methods also constitutes an essential part of this matter in order to validate the computation models and enable longitudinal monitoring. Finally, the current challenges of lifelike biohybrid materials, including reproducibility, ethical considerations, and translation, are discussed. Advances in the development of lifelike materials will open new biomedical horizons, where perhaps what is currently envisioned as science fiction will become a science‐driven reality in the future.

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Controlling Particle Fraction in Microporous Annealed Particle Scaffolds for 3D Cell Culture

Cited by 6 publications

References 23 publications

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds

Spatial Confinement Modulates Macrophage Response in Microporous Annealed Particle (MAP) Scaffolds

Engineering the microstructure and spatial bioactivity of MAP scaffolds in vitro instructs neovascularization in vivo

Lifelike Transformative Materials for Biohybrid Implants: Inspired by Nature, Driven by Technology

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