Controlling stem cell fate using cold atmospheric plasma

Abstract

The stem cell is the foundation of regenerative medicine and tissue engineering. Regulating specific stem cell fate, such as cell attachment, proliferation, differentiation, and even death, undergoes continuous development. Cold atmospheric plasma (CAP), the core technology of plasma medicine, is attracting tremendous attention due to its ability and versatility to manipulate various types of cells, including stem cells. Specifically, the direct and indirect applications of CAP in controlling cell fate are best exemplified by upfront irradiation of the stem cells and modification of the stem cell niche, respectively. This review will describe the recent advances in various CAP strategies, both direct and indirect, and their influence on the fate of healthy and cancer stem cells. Particular emphasis will be placed on the mechanism of connecting the physical and chemical cues carried by the plasma and biological changes presented by the cells, especially at the transcriptomic level. The ultimate goal is to exploit CAP's potential in regenerative medicine.

Multimodal treatment combining cold atmospheric plasma and acidic fibroblast growth factor for multi‐tissue regeneration

“…The Piezobrush compact handheld plasma devices (Relyon Plasma GmbH, Regensburg, Germany), PZ2 and PZ3, were used throughout the experiments 7 . They are based on the CeraPlas component and piezoelectric direct discharge (PDD) technology, in which low input voltage is transformed to create high electric field density (Figure 1).…”

“…The Piezobrush compact handheld plasma devices (Relyon Plasma GmbH, Regensburg, Germany), PZ2 and PZ3, were used throughout the experiments. 7 They are based on the CeraPlas component and piezoelectric direct discharge (PDD) technology, in which low input voltage is transformed to create high electric field density (Figure 1). The piezoelectric radiofrequency (RF) plasma generator provides several advantages, such as a high ionization speed, an effective RONS generation rate, multigas ignition, low power consumption, and no magnetic fields.…”

“…CAP’s main components include ions, electrons, metastables, photons, and electromagnetic fields. Upon reaction with ambient air, CAP forms a hierarchical cluster of reactive oxygen and nitrogen species (RONS) 7 . The key role of RONS in various biological and cellular pathways 8 has helped to push the study of CAP into the medical field.…”

Non-Thermal Atmospheric Pressure Plasma-Conditioned Root Dentin Promotes Attraction and Attachment of Primary Human Dental Pulp Stem Cells in Real-Time Ex Vivo

“…In regenerative medicine, NTAPP may allow a versatile range of applications involving the manipulation of various cell types and processes, including stem cell attachment, proliferation, differentiation, and even apoptosis [3]. Tan et al reported evidence of NTAPP improving stem cell attachment, proliferation, and differentiation [3].…”

“…In regenerative medicine, NTAPP may allow a versatile range of applications involving the manipulation of various cell types and processes, including stem cell attachment, proliferation, differentiation, and even apoptosis [3]. Tan et al reported evidence of NTAPP improving stem cell attachment, proliferation, and differentiation [3]. It activated proliferation of various mesoderm-derived human stem cells [4], and enhanced proliferation of adipose tissue-derived stem cells without affecting their stem cell properties [5].…”