Controlling structural properties of self-assembled oligonucleotide–mercaptohexanol monolayers

Kick, Alfred; Bönsch, Martin; Kummer, K.; Vyalikh, D. V.; Молодцов, С. Л.; Mertig, Michael

doi:10.1016/j.elspec.2009.04.002

Cited by 31 publications

(41 citation statements)

References 52 publications

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“…This discrepancy is only apparent, because different properties are monitored by electrochemical and spectroscopic methods. The electrochemical measurements rely on the behaviour of the redox marker and provide a stoichiometry around 5 strand on the basis of a total charge compensation [20]. The value determined experimentally is lower, indicating that the binding does not proceed from a single, non-specific electrostatic interaction mode between the two partners.…”

Section: Discussionmentioning

confidence: 99%

“…In this ideal case, the peak currents are proportional to the scan rate according to Eq. (4) and (5).…”

Section: Interaction At the Surfacementioning

confidence: 98%

“…From the point of view of electrochemical DNA analysis, an essential feature of DNA is the presence of the negatively charged phosphate groups within the backbone. On the one hand, electrostatic repulsions between DNA strands can be used to modulate the DNA surface coverage of a sensor [3][4][5][6][7][8] or the hybridisation efficiency [9]. On the other hand, electrostatic interactions between DNA and charged electroactive markers can be exploited to devise various electrochemical sensing strategies.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)6]3+ and an elongated thrombin binding aptamer G-quadruplex

Rache

Kejnovská

Buess‐Herman

et al. 2015

Electrochimica Acta

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Section: Discussionmentioning

confidence: 99%

“…In this ideal case, the peak currents are proportional to the scan rate according to Eq. (4) and (5).…”

Section: Interaction At the Surfacementioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)6]3+ and an elongated thrombin binding aptamer G-quadruplex

Rache

Kejnovská

Buess‐Herman

et al. 2015

Electrochimica Acta

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“…We omit the details here and refer to previously published results [15,21]. We omit the details here and refer to previously published results [15,21].…”

Section: Hybridization Kinetics and Mass Transportmentioning

confidence: 99%

Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

Danz

Kick

Sonntag

et al. 2011

Engineering in Life Sciences

Self Cite

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The development of a surface plasmon resonance (SPR) spectrometer comprising angular-resolved analysis for quasi-monochromatic illumination is reported. The optical system utilizes disposable, injection-molded chips combined with a lateral imaging optical system for parallel analysis of one-dimensional spot arrays. Further parallelization is achieved by introducing a segmented light source. This source sequentially illuminates three neighbored one-dimensional arrays in order to keep angular-resolved analysis without introducing any mechanically moving parts. This system is applied to detect genetic variations among different DNA samples obtained from polymerase chain reaction (PCR). For this purpose, 135 spots on the chip surface have been prepared by spotting and analyzed separately

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“…For example Steel et al reported that longer DNA probes tend to orient themselves as coils lying on the surface rather than aligning perpendicularly [4]. Nonspecific attachment of the DNA chains to the surface via their negatively charged backbone is also a problem which can be eliminated by utilizing the mentioned molecule blocking agents [5]. Sakao and Nakamura also reported a novel approach to apply DNA probes with a lower double stranded enhancement region [6].…”

Section: Introductionmentioning

confidence: 99%

AFM nanoshaving: A novel prospect for the structural comparison of bioreceptor layers

Bonyár

Harsányi

2011

Proceedings of the 2011 34th International Spring Seminar on Electronics Technology (ISSE)

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In this work we present a nanoscale method called AFM nanoshaving (atomic force microscopy) as a prospect for the structural comparison of bioreceptor layers. Upon nanoshaving a biofunctionalized surface -such as DNA receptor chains on a gold thin film substrate -a higher pressing force is applied with the AFM tip during the scan, which removes the previously chemically attached bioreceptor molecules. By analyzing the cross sections of the shaved and untouched regions of the surface it is possible to gain information about the orientation of the receptor molecules which is not possible by any other indirect measurement methods. Our work discusses the possibilities of this novel technique and presents pilot results about the characterization of DNA based bioreceptor layers with AFM nanoshaving. 978-1-4577-2112-0/2011/$ 26.00

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Controlling structural properties of self-assembled oligonucleotide–mercaptohexanol monolayers

Cited by 31 publications

References 52 publications

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)6]3+ and an elongated thrombin binding aptamer G-quadruplex

Electrochemical and circular dichroism spectroscopic evidence of two types of interaction between [Ru(NH3)6]3+ and an elongated thrombin binding aptamer G-quadruplex

Surface plasmon resonance platform technology for multi‐parameter analyses on polymer chips

AFM nanoshaving: A novel prospect for the structural comparison of bioreceptor layers

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