Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout

Dong, Ning; Cai, Qingyun; Li, Zhixin; Xu, Liangzhen; Wu, Huping; Zhong, Lin; Qiu, Bin; Li, Cheng; Lin, Zhenyu

doi:10.1039/d1an00257k

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2021

DOI: 10.1039/d1an00257k

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Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout

Ning Dong

Qingyun Cai

Zhixin Li

et al.

Abstract: The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection. In this...

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Cited by 4 publications

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“…Hence, measuring HAase through a high-accuracy method is essential. Many methods, including fluorescent methods, − colorimetric methods, weighting methods, and so on, have been reported for the HAase quantification so far. Our group has developed two ECL methods for HAase detection based on changing the molecular weight of signal probes by HAase for signal probe ultrafiltration and collection and decomposing the hydrogel loaded with signal probes by HAase for signal probe release .…”

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confidence: 99%

Electrochemiluminescence Biosensor for Hyaluronidase Based on the Adjustable Electrostatic Interaction between the Surface-Charge-Controllable Nanoparticles and Negatively Charged Electrode

Huang

Liu

et al. 2022

ACS Sens.

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A novel electrochemiluminescence (ECL) biosensor for hyaluronidase (HAase) based on the adjustable electrostatic interaction between the surface-charge-controllable nanoparticles and negatively charged electrode has been devised. Hyaluronic acid (HA)-coated amino-modified ruthenium bipyridine-doped silica nanoparticles (Ru@SiO 2 −NH 2 @HA NPs) have been synthesized and act as ECL indicators, and the surface of this particle is negatively charged because HA contains a large amount of OH− and COO−. The strong electrostatic repulsion between the Ru@ SiO 2 −NH 2 @HA NPs and negatively charged indium tin oxide (ITO) electrode surface leads to the detection of a low-intensity ECL signal. In the presence of HAase, the HA on the surface of the Ru@SiO 2 −NH 2 @HA NPs can be decomposed, and the particles can be transformed into positively charged amino-modified ruthenium bipyridine-doped silica nanoparticles (Ru@SiO 2 −NH 2 NPs), which can be concentrated near the surface of the ITO electrode through electrostatic attraction, and result in the detection of an enhanced ECL signal. The ECL of the system has a good linear relationship with HAase concentration in the range of 2.0−60 U/ mL, and the limit of detection was 0.37 U/mL. The designed biosensor had been applied to detect the target in real samples with satisfied results.

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confidence: 99%

Electrochemiluminescence Biosensor for Hyaluronidase Based on the Adjustable Electrostatic Interaction between the Surface-Charge-Controllable Nanoparticles and Negatively Charged Electrode

Huang

Liu

et al. 2022

ACS Sens.

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New sensing methods using commercially available products: Based on PGM and PTS

Jiang,

Deng,

et al. 2025

Biosensors and Bioelectronics

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Patent Overview of Innovative Hyaluronic Acid-Based Hydrogel Biosensors

Fatimi,

Damiri,

Berrada

et al. 2024

Biosensors

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Hyaluronic acid-based hydrogels are emerging as highly versatile materials for cost-effective biosensors, capable of sensitive chemical and biological detection. These hydrogels, functionalized with specific groups, exhibit sensitivity modulated by factors such as temperature, pH, and analyte concentration, allowing for a broad spectrum of applications. This study presents a patent-centered overview of recent advancements in hyaluronic acid hydrogel biosensors from 2003 to 2023. A total of 50 patent documents—including 41 patent applications and 9 granted patents—reveal a growing interest, primarily driven by United States-based institutions, which account for approximately 54% of all filings. This trend reflects the strong collaboration between universities, industry, and foundations in pushing this technology forward. Most patented technologies focus on biosensors for in vivo blood analysis, measuring critical parameters such as gas concentration and pH, with particular emphasis on glucose monitoring via tissue impedance using enzyme-immobilized oxidase electrodes. Additionally, the 9 granted patents collectively showcase key innovations, highlighting applications from continuous glucose monitors to implantable vascular devices and sweat analyte detection systems. These patents underscore the adaptability and biocompatibility of hyaluronic acid hydrogels, reinforcing their role in enhancing biosensor performance for real-time health monitoring. In summary, this overview highlights the importance of patent analysis in tracking and directing research and development, helping to clarify the field’s evolution and identify innovation gaps for hyaluronic acid-based hydrogel biosensors.

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Convenient hyaluronidase biosensors based on the target-trigger enhancing of the permeability of a membrane using an electronic balance as a readout

Abstract: The change in hyaluronidase (HAase) is related to specific changes in the structure of vitreous, and it is necessary to develop simple but sensitive methods for HAase detection. In this...

Cited by 4 publications

References 31 publications

Electrochemiluminescence Biosensor for Hyaluronidase Based on the Adjustable Electrostatic Interaction between the Surface-Charge-Controllable Nanoparticles and Negatively Charged Electrode

Electrochemiluminescence Biosensor for Hyaluronidase Based on the Adjustable Electrostatic Interaction between the Surface-Charge-Controllable Nanoparticles and Negatively Charged Electrode

New sensing methods using commercially available products: Based on PGM and PTS

Patent Overview of Innovative Hyaluronic Acid-Based Hydrogel Biosensors

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