Conventional and molecular biotyping of Brucella strains isolated from cattle, sheep and human

AYDIN, İÇA Tuba ;

doi:10.1501/vetfak_0000002536

Ankara Üniversitesi Veteriner Fakültesi Dergisi

2012

DOI: 10.1501/vetfak_0000002536

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Conventional and molecular biotyping of Brucella strains isolated from cattle, sheep and human

İÇA Tuba ; AYDIN¹

Abstract: Summary:In this study, the role of Brucella spp. in cattle and sheep abortions among Kayseri region was investigated and predominant subspecies and biovars in this region were determined by conventional and molecular biotyping methods. For this purpose, 61 cattle and 64 sheep abortion material and also 50 human blood isolates were examined. A total of 29 Brucella spp. 17 (27.9%) and 12 (18.7%) of which were isolated from cattle and sheep specimens, respectively) were isolated from animal sources. Both animal a… Show more

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Cited by 6 publications

References 18 publications

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Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

Şevik

2020

Transbounding Emerging Dis

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This study was carried out to investigate the frequency and genetic diversity of pestiviruses in abortion cases in cattle and small ruminants in Turkey. During January 2012 and December 2017, a total of 2029 aborted foetuses (553 bovine foetuses, 1,388 sheep foetuses and 88 goat foetuses) were collected from different regions of Turkey. Real-time RT-PCR (RRT-PCR) assays were used to detect pestiviral RNA in aborted foetuses. To confirm the cause of abortion, pestivirus-positive foetuses were also examined for the presence of Brucella spp., Campylobacter spp., Chlamydophila abortus (C. abortus), akabane virus, bluetongue virus and Schmallenberg virus by molecular detection methods. Pestiviral RNA was detected in 61 (11%) of the 553 bovine foetuses, 124 (8.9%) of the 1,388 sheep foetuses and 3 (3.4%) of the 88 goat foetuses. Furthermore, C. abortus DNA was detected in 3 pestivirus-positive sheep foetuses, whereas other infectious agents were not detected in pestivirus-positive foetuses. Genetic characterization of the pestivirus RRT-PCR positive samples was conducted by sequencing 5′ untranslated (5′ UTR) and non-structural autoprotease (N pro ) genomic regions. A total of 68 sequences were obtained, and phylogenetic analyses revealed that all sequences belonged to BVDV-1, including 1b (8/68), 1f(2/68), 1l (4/68), 1r (10/68), Aydin-like pestivirus (20/68) and one unknown genotype (24/68). The 5′ UTR and N pro sequences of this unknown genotype differed from pestiviruses previously described, providing evidence for the presence of an emerging genotype within the species Pestivirus I, tentatively named as 'Konya-like' pestivirus. 'Konya-like' pestivirus was the dominant genotype in sheep foetuses, whereas Aydin-like pestivirus was found to be the predominant genotype in bovine foetuses.To the best my knowledge, this is the first report of Aydin-like pestivirus infection in cattle. The information provided in this study contributes to the understanding the dissemination and evolution of pestiviruses and could be beneficial for developing more effective vaccines.

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Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

Şevik

2020

Transbounding Emerging Dis

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Isolation of Brucella melitensis strains from Syrian bovine milk samples

Al-Mariri¹

2015

BJVM

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SummaryAl-Mariri, A., 2015. Isolation of Brucella melitensis strains from Syrian bovine milk samples. Bulg. J. Vet. Med., 18, No 1, 4048. Brucellosis is a worldwide zoonotic disease that still constitutes a major public health problem in Syria. In the current study, Brucella has been isolated from cow milk samples and typed by both molecular and conventional techniques. Over a 6-year period (2002)(2003)(2004)(2005)(2006)(2007) 2372 milk samples were collected from Syrian cow herds. The milk ring test (MRT) was performed where 57% of samples were found to be positive. Milk samples were cultured on the Brucella selective medium and incubated for 24-48 h. One-quarter of milk samples were contaminated with Brucella, which is considered as a relatively high rate of contamination. In addition, biochemical properties of isolates have been identified. All isolated Brucella strains were positive for catalase, oxidase and urea reaction, did not need CO 2 to grow and rarely produced H 2 S, could grow in the presence of fuchsin and thionin. The agglutination test was negative with anti-R and positive with anti-M and anti-A sera; so it was determined as B. melitensis strain, biovar 3. A specific PCR assay targeting the bcsp31 gene and the specific integration of IS711 elements within the genome of Brucella species has been used. Molecular typing results were compatible with conventional methods.

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Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays

Kaur

Sharma

Arora

et al. 2017

IJAR

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The present study was carried out to evaluate the conventional and molecular techniques for diagnosis of bovine brucellosis. A total of four isolates of Brucella abortus obtained from 100 clinical samples of foetal stomach contents, vaginal mucus and uterine discharges were characterized biochemically. The isolates were confirmed as Brucella spp. by PCR using B4/B5 primer pair and as B. abortus by Bruce Ladder multiplex PCR. By Hinic Real-time PCR, all the four isolates were confirmed as Brucella spp. with C t values between 14-16. With DNA extracted from 40 clinical samples of foetal stomach contents, vaginal mucus and uterine discharges, Real time PCR appeared most sensitive of the three molecular and conventional techniques as it detected maximum number of positive samples.

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Conventional and molecular biotyping of Brucella strains isolated from cattle, sheep and human

Cited by 6 publications

References 18 publications

Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

Genomic characterization of pestiviruses isolated from bovine, ovine and caprine foetuses in Turkey: A potentially new genotype of Pestivirus I species

Isolation of Brucella melitensis strains from Syrian bovine milk samples

Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays

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