Conversion of AML-blasts to leukemia-derived dendritic cells (DCleu) in ‘DC-culture-media’ shifts correlations of released chemokines with antileukemic T-cell reactions

Merle, M.; Fischbacher, D.; Liepert, Anja; Grabrucker, Christine; Kroell, Tanja; Kremser, Andreas; Dreyssig, Julia; Freudenreich, M.; Schuster, Friedhelm R.; Kraemer, Doris; Koehne, Claus-Henning; Kolb, Hans Jochem; Schmid, Christoph; Schmetzer, Helga

doi:10.1016/j.imbio.2021.152088

Cited by 3 publications

(5 citation statements)

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“…To monitor AML patients’ disease or antileukemic immunoreactions, analyses of different cellular, humoral, and soluble factors are important [ 12 , 15 , 22 , 24 , 25 , 26 ]. VOCs released by leukemic or immune cells’ processes and measured directly by the eNose might be an important tool to monitor biomarkers related to the disease, as previously shown in VOC studies with other diseases [ 31 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…Analyses of different cellular/humoral, soluble factors [ 8 , 15 , 21 , 22 , 23 , 24 ] or circulating vesicles (e.g., extracellular vesicles (EVs) [ 26 , 27 ]) were tested with respect to a refined monitoring of immunological or tumor-associated processes. VOC differentiation of healthy and leukemic serum or culture samples might be a new strategy for screening recurrence and quantifying the tumor load of diseased patients.…”

Section: Discussionmentioning

confidence: 99%

“…With the advent of inexpensive, environmentally friendly, and biocompatible sensor systems, health monitoring using VOCs may transform laborious or invasive procedures that currently can only be used in facilities specialized for this purpose into a technology that can be used anywhere and anytime by individuals. Analyses of different cellular/humoral, soluble factors [8,15,[21][22][23][24] or circulating vesicles (e.g., extracellular vesicles (EVs) [26,27]) were tested with respect to a refined monitoring of immunological or tumor-associated processes. VOC differentiation of healthy and leukemic serum or culture samples might be a new strategy for screening recurrence and quantifying the tumor load of diseased patients.…”

Section: Strengths and Limitations Of Voc Analysesmentioning

confidence: 99%

“…Analyses of different (activating or inhibitory) cellular/humoral, soluble factors, or even the smallest molecules could contribute to our understanding of leukemia-related as well as antileukemic processes [ 8 , 15 , 21 , 22 , 23 , 24 ]. In recent years, the role of physical factors (e.g., physiological hypoxia [ 25 ] or circulating vesicles (e.g., extracellular vesicles (EVs) [ 26 , 27 ]) has been tested with respect to a refined monitoring of immunological or tumor-associated processes.…”

Section: Introductionmentioning

confidence: 99%

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Volatile Phases Derived from Serum, DC, or MLC Culture Supernatants to Deduce a VOC-Based Diagnostic Profiling Strategy for Leukemic Diseases

Baudrexler

Boeselt

et al. 2023

Biomolecules

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Volatile organic compounds (VOCs) reflect the metabolism in healthy and pathological conditions, and can be collected easily in a noninvasive manner. They are directly measured using electronical nose (eNose), and may qualify as a systemic tool to monitor biomarkers related to disease. Myeloid leukemic blasts can be transformed into leukemia-derived dendritic cells (DCleu) able to improve (anti-leukemic) immune responses. To profile immunological changes in healthy and acute myeloid leukemic (AML) patients’ ex vivo cell cultures, we correlated the cell biological data with the profiles of cell culture supernatant-derived VOCs. DC/DCleu from leukemic or healthy whole blood (WB) were generated without (Control) or with immunomodulatory Kit M (Granulocyte macrophage-colony-stimulating-factor (GM-CSF) + prostaglandin E1 (PGE1)) in dendritic cell cultures (DC culture). Kit-pretreated/not pretreated WB was used to stimulate T cell-enriched immunoreactive cells in mixed lymphocyte cultures (MLC culture). Leukemia-specific adaptive and innate immune cells were detected with a degranulation assay (Deg) and an intracellular cytokine assay (InCyt). Anti-leukemic cytotoxicity was explored with a cytotoxicity fluorolysis assay (CTX). VOCs collected from serum or DC- and MLC culture supernatants (with vs. without Kit M pretreatment and before vs. after culture) were measured using eNose. Compared to the Control (without treatment), Kit M-pretreated leukemic and healthy WB gave rise to higher frequencies of mature (leukemia-derived) DC subtypes of activated and (memory) T cells after MLC. Moreover, antigen (leukemia)-specific cells of several lines (innate and adaptive immunity cells) were induced, giving rise to blast-lysing cells. The eNose could significantly distinguish between healthy and leukemic patients’ serum, DC and MLC culture supernatant-derived volatile phases and could significantly separate several supernatant (with vs. without Kit M treatment, cultured vs. uncultured)-derived VOCs within subgroups (healthy DC or leukemic DC, or healthy MLC or leukemic MLC supernatants). Interestingly, the eNose could indicate a Kit M- and culture-associated effect. The eNose may be a prospective option for the deduction of a VOC-based profiling strategy using serum or cell culture supernatants and could be a useful diagnostic tool to recognize or qualify AML disease.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Strengths and Limitations Of Voc Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Volatile Phases Derived from Serum, DC, or MLC Culture Supernatants to Deduce a VOC-Based Diagnostic Profiling Strategy for Leukemic Diseases

Baudrexler

Boeselt

et al. 2023

Biomolecules

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show abstract

“…Understanding both leukemia-related and anti-leukemic processes requires the analysis of various cellular and humoral factors, whether activating or inhibitory, as well as soluble factors and even small molecules [13,18,[21][22][23][24][25]. In recent years, the role of physical factors, such as physiological hypoxia [26] and circulating vesicles (e.g., extracellular vesicles (EVs)) [27,28], has been investigated for their potential in refined monitoring immunological or tumorassociated processes.…”

Section: Methodological Tools For Monitoring Aml and Anti-leukemic Pr...mentioning

confidence: 99%

Volatile Profiling using an eNose - Exploiting Breath Volatile Organic Compounds for Disease Monitoring in Refractory Acute Myeloid Leukemia Patients

2024

Ann Case Report

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Influence of probiotics on the periodontium, the oral microbiota and the immune response during orthodontic treatment in adolescent and adult patients (ProMB Trial): study protocol for a prospective, double-blind, controlled, randomized clinical trial

et al. 2022

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Background Orthodontic treatment with fixed appliances is often necessary to correct malocclusions in adolescence or adulthood. However, oral hygiene is complicated by appliances, and prior studies indicate that they may trigger oral inflammation and dysbiosis of the oral microbiota, especially during the first 3 months after insertion, and, thus, may present a risk for inflammatory oral diseases. In recent periodontal therapeutic studies, probiotics have been applied to improve clinical parameters and reduce local inflammation. However, limited knowledge exists concerning the effects of probiotics in orthodontics. Therefore, the aim of our study is to evaluate the impact of probiotics during orthodontic treatment. Methods This study is a monocentric, randomized, double blind, controlled clinical study to investigate the effectiveness of daily adjuvant use of Limosilactobacillus reuteri (Prodentis®-lozenges, DSM 17938, ATCC PTA 5289) versus control lozenges during the first three months of orthodontic treatment with fixed appliances. Following power analysis, a total of 34 adolescent patients (age 12–17) and 34 adult patients (18 years and older) undergoing orthodontic treatment at the University Hospital Erlangen will be assigned into 2 parallel groups using a randomization plan for each age group. The primary outcome measure is the change of the gingival index after 4 weeks. Secondary outcomes include the probing pocket depth, the modified plaque index, the composition of the oral microbiota, the local cytokine expression and—only for adults—serum cytokine levels and the frequencies of cells of the innate and adaptive immune system in peripheral blood. Discussion Preventive strategies in everyday orthodontic practice include oral hygiene instructions and regular dental cleaning. Innovative methods, like adjuvant use of oral probiotics, are missing. The aim of this study is to analyse, whether probiotics can improve clinical parameters, reduce inflammation and prevent dysbiosis of the oral microbiota during orthodontic treatment. If successful, this study will provide the basis for a new strategy of prophylaxis of oral dysbiosis-related diseases during treatment with fixed appliances. Trial registration This trial is registered at ClinicalTrials.gov in two parts under the number NCT04598633 (Adolescents, registration date 10/22/2020), and NCT04606186 (Adults, registration date 10/28/2020).

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Conversion of AML-blasts to leukemia-derived dendritic cells (DCleu) in ‘DC-culture-media’ shifts correlations of released chemokines with antileukemic T-cell reactions

Cited by 3 publications

References 76 publications

Volatile Phases Derived from Serum, DC, or MLC Culture Supernatants to Deduce a VOC-Based Diagnostic Profiling Strategy for Leukemic Diseases

Volatile Phases Derived from Serum, DC, or MLC Culture Supernatants to Deduce a VOC-Based Diagnostic Profiling Strategy for Leukemic Diseases

Volatile Profiling using an eNose - Exploiting Breath Volatile Organic Compounds for Disease Monitoring in Refractory Acute Myeloid Leukemia Patients

Influence of probiotics on the periodontium, the oral microbiota and the immune response during orthodontic treatment in adolescent and adult patients (ProMB Trial): study protocol for a prospective, double-blind, controlled, randomized clinical trial

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