Abstract:In this study, we extracted exosomes from cooked meat by ultra-high-speed centrifugation. Exosome had an average of size of 70.29 nm. Flow cytometry demonstrated that the positive rate of exosomal surface marker CD63 and CD81 were 84.5% and 95.9%. microRNA sequencing revealed the exosomal microRNA were differences among porcine muscle, fat and liver. The mice plasma levels of miR-1, miR-133a-3p, miR-206 and miR-99a were increase with varying degrees after drinking water with exosomes. GTT and ITT suggest that … Show more
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