Coordinated cortical ER remodeling facilitates actomyosin ring assembly

Zhang, Dan; See, Tingyi

doi:10.1016/j.cub.2022.04.086

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Upon cytokinesis, septins split into double rings, and contractile actomyosin rings are formed between the double rings ( 13 , 34 ). As actomyosins exclusively bind to the ER-unbound PM at least in fission yeasts ( 8 , 36 ), the ER-free PM maintained by septins would help efficient contractile ring assembly, although we note that the requirement of intact septin double rings for cytokinesis is argued ( 61 , 62 ).…”

Section: Discussionmentioning

confidence: 90%

“…This raised the possibility that the ER was detached from the PM by machineries that were commonly utilized in the polarity establishment and/or maintenance at these sites. The PM-associated ER is detached from the PM at sites with active exocytosis in fission yeasts, although how exocytosis remodels the PM-associated ER is unclear ( 7 , 36 ). Because exocytosis is also highly active at the polarity sites of budding yeasts ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Exocytic plasma membrane flows remodel endoplasmic reticulum–plasma membrane tethering for septin collar assembly

Sugiyama,

Kono

2024

Sci. Adv.

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Endoplasmic reticulum (ER)–plasma membrane (PM) tethering is crucial for the non-vesicular lipid transport between the ER membrane and the PM. However, the PM-associated ER can impede the PM binding of cytoskeletons and other organelles. It is poorly understood how the competition between the ER and cytoskeletons/organelles on the PM is resolved. Here, we show that, upon septin collar assembly, ER-PM tethering proteins are excluded from the yeast bud sites, and the PM-associated ER is locally detached from the PM. Our results suggest that PM flows by polarized exocytosis extrude PM proteins, including ER-PM tethering proteins, from the bud sites. When the reorganization of the ER-PM tethering was inhibited by exocytosis repression, septin localization was restricted to the PM sites poor in ER-PM tethering proteins. This study proposes machinery reconciling ER-septin competition on the PM, providing mechanistic insights into the spatial organization of PM-associated organelles and cytoskeletons.

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Section: Discussionmentioning

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Exocytic plasma membrane flows remodel endoplasmic reticulum–plasma membrane tethering for septin collar assembly

Sugiyama,

Kono

2024

Sci. Adv.

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VAP-mediated membrane-tethering mechanisms implicate ER-PM contact function in pH homeostasis

Hoh,

Mu,

See

et al. 2024

Cell Reports

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Fission yeast Duc1 links to ER–PM contact sites and influences PM lipid composition and cytokinetic ring anchoring

Willet,

Park,

Snider

et al. 2024

Journal of Cell Science

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Cytokinesis is the final stage of the cell cycle that results in the physical separation of daughter cells. To accomplish cytokinesis, many organisms build an actin- and myosin-based cytokinetic ring (CR) that is anchored to the plasma membrane (PM). Defects in CR–PM anchoring can arise when the PM lipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] is depleted. In Schizosaccharomyces pombe, reduced PM PI(4,5)P2 results in a CR that cannot maintain a medial position and slides toward one cell end, resulting in two differently sized daughter cells. S. pombe PM PI(4,5)P2 is synthesized by the phosphatidylinositol 4-phosphate 5-kinase (PI5-kinase) Its3, but what regulates this enzyme to maintain appropriate PM PI(4,5)P2 levels in S. pombe is not known. To identify Its3 regulators, we used proximity-based biotinylation, and the uncharacterized protein Duc1 was specifically detected. We discovered that Duc1 decorates the PM except at the cell division site and that its unique localization pattern is dictated by binding to the endoplasmic reticulum (ER)–PM contact site proteins Scs2 and Scs22. Our evidence suggests that Duc1 also binds PI(4,5)P2 and helps enrich Its3 at the lateral PM, thereby promoting PM PI(4,5)P2 synthesis and robust CR–PM anchoring.

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Coordinated cortical ER remodeling facilitates actomyosin ring assembly

Cited by 3 publications

References 32 publications

Exocytic plasma membrane flows remodel endoplasmic reticulum–plasma membrane tethering for septin collar assembly

Exocytic plasma membrane flows remodel endoplasmic reticulum–plasma membrane tethering for septin collar assembly

VAP-mediated membrane-tethering mechanisms implicate ER-PM contact function in pH homeostasis

Fission yeast Duc1 links to ER–PM contact sites and influences PM lipid composition and cytokinetic ring anchoring

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