COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

An, Yuyan; Li, Jing; Feng, Yuxin; Sun, Zhi-Mao; Li, Zhongqi; Wang, Xiaoting; Zhang, Meixiang; He, Junyu

doi:10.3390/ijms232315037

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…S4 ). For instance, the well-known regulator of flowering and stomatal opening SUPPRESSOR OF OVEREXPRESSION OF CO 1 ( SOC1 , also named AGAMOUS-LIKE 20 [ AGL20 ]) ( 29 – 33 ) showed increased intron retention ratio within the nucleus upon exposure to light, indicating that SOC1 transcripts are post-transcriptionally spliced by light ( Fig. 1 E and SI Appendix , Fig.…”

Section: Resultsmentioning

confidence: 99%

Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5

Yan,

Luo,

Qin

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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Light plays a central role in plant growth and development, providing an energy source and governing various aspects of plant morphology. Previous study showed that many polyadenylated full-length RNA molecules within the nucleus contain unspliced introns (post-transcriptionally spliced introns, PTS introns), which may play a role in rapidly responding to changes in environmental signals. However, the mechanism underlying post-transcriptional regulation during initial light exposure of young, etiolated seedlings remains elusive. In this study, we used FLEP-seq2, a Nanopore-based sequencing technique, to analyze nuclear RNAs in Arabidopsis ( Arabidopsis thaliana ) seedlings under different light conditions and found numerous light-responsive PTS introns. We also used single-nucleus RNA sequencing (snRNA-seq) to profile transcripts in single nucleus and investigate the distribution of light-responsive PTS introns across distinct cell types. We established that light-induced PTS introns are predominant in mesophyll cells during seedling de-etiolation following exposure of etiolated seedlings to light. We further demonstrated the involvement of the splicing-related factor A. thaliana PROTEIN ARGININE METHYLTRANSFERASE 5 (AtPRMT5), working in concert with the E3 ubiquitin ligase CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1), a critical repressor of light signaling pathways. We showed that these two proteins orchestrate light-induced PTS events in mesophyll cells and facilitate chloroplast development, photosynthesis, and morphogenesis in response to ever-changing light conditions. These findings provide crucial insights into the intricate mechanisms underlying plant acclimation to light at the cell-type level.

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Section: Resultsmentioning

confidence: 99%

Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5

Yan,

Luo,

Qin

et al. 2024

Proc. Natl. Acad. Sci. U.S.A.

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“…In addition, NO is a key player in the signal transduction process of dark-induced Arabidopsis stomatal closure. COP1 transduced H 2 O 2 signaling and promoted NO accumulation in guard cells by suppressing FT , TSF and SOC1 expression levels, thus consequently leading to stomatal closure in darkness [ 16 ]. These findings add new insights into the mechanisms of dark-induced stomatal closure.…”

mentioning

confidence: 99%

Advances in Nitric Oxide Signalling and Metabolism in Plants

Liao

Igamberdiev

Palma

2023

IJMS

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Integration of light quality signals regulates ABA abundance and stomatal movements during seedling establishment

Gustavsson,

Hill,

Franklin

et al. 2024

Preprint

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Obtaining sufficient light for photosynthesis and avoiding desiccation are two key challenges faced by seedlings during early establishment. Perception of light quality via specialised photoreceptors signals the availability of sunlight for photosynthesis. Canopy shade is depleted in red (R) and enriched in far-red (FR) light, lowering R:FR ratio, while direct sunlight and sunflecks contain UV-B. The balance of these wavelengths can determine the developmental strategy adopted by seedlings to either avoid shade, via stem elongation, or promote the expansion of photosynthetic organs. How seedlings regulate stomatal movements in different light environments is poorly understood. Using FR and UV-B supplementation to mimic aspects of canopy shade and sunlight respectively, we monitored stomatal apertures in Arabidopsis thaliana cotyledons. We show that low R:FR inhibits stomatal opening via a mechanism involving PHYTOCHROME INTERACTING FACTOR 4 (PIF4) and increased abscisic acid (ABA). In contrast, UV-B perceived by the UV RESISTANCE LOCUS 8 (UVR8) photoreceptor antagonises this response to promote stomatal opening in a response requiring phototropin photoreceptors. The convergence of phytochrome and UVR8 signalling to control ABA abundance enables plants to coordinate stem elongation and water use during seedling establishment in dynamic light environments.

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COP1 Mediates Dark-Induced Stomatal Closure by Suppressing FT, TSF and SOC1 Expression to Promote NO Accumulation in Arabidopsis Guard Cells

Cited by 3 publications

References 33 publications

Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5

Light controls mesophyll-specific post-transcriptional splicing of photoregulatory genes by AtPRMT5

Advances in Nitric Oxide Signalling and Metabolism in Plants

Integration of light quality signals regulates ABA abundance and stomatal movements during seedling establishment

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