Copalyl Diphosphate Synthase Mutation Improved Salt Tolerance in Maize (Zea mays. L) via Enhancing Vacuolar Na+ Sequestration and Maintaining ROS Homeostasis

Zhang, Yushi; Wang, Yubin; Xing, Jiapeng; Wan, Jiachi; Wang, Xilei; Zhang, Juan; Wang, Xiaodong; Li, Zhaohu; Zhang, Mingcai

doi:10.3389/fpls.2020.00457

Cited by 13 publications

(11 citation statements)

References 63 publications

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“…A total of 12 seedlings were planted in each box. As described in our previous studies, the nutrient solution comprised 0.75 mM K 2 SO 4 , 0.65 mM MgSO 4 , 2 mM Ca(NO 3 ) 2 , 0.1 mM KCl, 0.25 mM KH 2 PO 4 , 0.1 mM Fe-EDTA, 1.0 mM H 3 BO 3 , 1 mM MnSO 4 , 1 mM ZnSO 4 , 0.1 mM CuSO 4 , and 0.05 mM (NH 4 ) 6 MoO 24 (Zhang et al, 2016;Zhang et al, 2020). The nutrient solution was replaced every 3 days until the plants were harvested.…”

Section: Plant Cultivation and Treatmentsmentioning

confidence: 99%

“…After two days, 125 mM NaCl was added to half of the pots in each treatment group. The concentration and duration of treatment with GB was selected according to the results of preliminary experiments, and the concentration and duration of treatment with NaCl was based on our previous study on the same inbred line (Zhang et al, 2020). The entire treatment was continued for 13 days.…”

Section: Plant Cultivation and Treatmentsmentioning

confidence: 99%

“…The cellular Na + content of 9-day NaCl-treated leaves was determined using the Na + indicator dye, CoroNa Green (Invitrogen, United Kingdom). Sample collection and measurement was performed as previously described by Zhang et al (2020) with some modifications. The lower epidermis of the third leaf was peeled off to expose the mesophyll cells, which were allowed to adhere to the staining solution.…”

Section: Cellular Na + Imaging and Measurement Of Na + Content With C...mentioning

confidence: 99%

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Glycine betaine increases salt tolerance in maize (Zea mays L.) by regulating Na+ homeostasis

Zhu

Li²,

Zhang³

et al. 2022

Front. Plant Sci.

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Improving crop salt tolerance is an adaptive measure to climate change for meeting future food demands. Previous studies have reported that glycine betaine (GB) plays critical roles as an osmolyte in enhancing plant salt resistance. However, the mechanism underlying the GB regulating plant Na+ homeostasis during response to salinity is poorly understood. In this study, hydroponically cultured maize with 125 mM NaCl for inducing salinity stress was treated with 100 μM GB. We found that treatment with GB improved the growth of maize plants under non-stressed (NS) and salinity-stressed (SS) conditions. Treatment with GB significantly maintained the properties of chlorophyll fluorescence, including Fv/Fm, ΦPSII, and ΦNPQ, and increased the activity of the antioxidant enzymes for mitigating salt-induced growth inhibition. Moreover, GB decreased the Na+/K+ ratio primarily by reducing the accumulation of Na+ in plants. The results of NMT tests further confirmed that GB increased Na+ efflux from roots under SS condition, and fluorescence imaging of cellular Na+ suggested that GB reduced the cellular allocation of Na+. GB additionally increased Na+ efflux in leaf protoplasts under SS condition, and treatment with sodium orthovanadate, a plasma membrane (PM) H+-ATPase inhibitor, significantly alleviated the positive effects of GB on Na+ efflux under salt stress. GB significantly improved the vacuolar activity of NHX but had no significant effects on the activity of V type H+-ATPases. In addition, GB significantly upregulated the expression of the PM H+-ATPase genes, ZmMHA2 and ZmMHA4, and the Na+/H+ antiporter gene, ZmNHX1. While, the V type H+-ATPases gene, ZmVP1, was not significantly regulated by GB. Altogether these results indicate that GB regulates cellular Na+ homeostasis by enhancing PM H+-ATPases gene transcription and protein activities to improve maize salt tolerance. This study provided an extended understanding of the functions of GB in plant responses to salinity, which can help the development of supportive measures using GB for obtaining high maize yield in saline conditions.

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Section: Plant Cultivation and Treatmentsmentioning

confidence: 99%

Section: Plant Cultivation and Treatmentsmentioning

confidence: 99%

Section: Cellular Na + Imaging and Measurement Of Na + Content With C...mentioning

confidence: 99%

See 1 more Smart Citation

Glycine betaine increases salt tolerance in maize (Zea mays L.) by regulating Na+ homeostasis

Zhu

Li²,

Zhang³

et al. 2022

Front. Plant Sci.

Self Cite

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“…Cytochrome P450 monooxygenases ( OsCYP71D8L ) overexpression, a GA catabolic gene, enhances salt tolerance by reducing GA content and maintaining high chlorophyll and soluble sugar contents in rice (Zhou et al, 2018). Likewise, maize mutant lines having a mutation in the ent‐ copalyl diphosphate synthase, a GA biosynthetic enzyme showed dwarf growth, delayed senescence of leaf accompanied by reduced GA content under salt stress (Zhang et al, 2020). Also, the mutant lines reported high activity of antioxidant enzymes, increased content of soluble sugars, and Na + sequestration, thereby improving salt tolerance in maize (Zhang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, maize mutant lines having a mutation in the ent‐ copalyl diphosphate synthase, a GA biosynthetic enzyme showed dwarf growth, delayed senescence of leaf accompanied by reduced GA content under salt stress (Zhang et al, 2020). Also, the mutant lines reported high activity of antioxidant enzymes, increased content of soluble sugars, and Na + sequestration, thereby improving salt tolerance in maize (Zhang et al, 2020). Altogether, GA accumulation and signaling are crucial for seed germination, whereas GA level reduction and signaling are necessary to mitigate salt stress.…”

Section: Introductionmentioning

confidence: 99%

Hormonal crosstalk in regulating salinity stress tolerance in graminaceous crops

Choudhary

Pramitha

Rana

et al. 2021

Physiologia Plantarum

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Soil salinity is one of the major threats that pose challenges to global cereal productivity and food security. Cereals have evolved sophisticated mechanisms to circumvent stress at morpho-physiological, biochemical, and molecular levels. Salt stress cues are perceived by the roots, which trigger the underlying signaling pathways that involve phytohormones. Each phytohormone triggers a specific signaling pathway integrated in a complex manner to produce antagonistic, synergistic, and additive responses. Phytohormones induce salt-responsive signaling pathways to modulate various physiological and anatomical mechanisms, including cell wall repair, apoplastic pH regulation, ion homeostasis, root hair formation, chlorophyll content, and leaf morphology. Exogenous applications of phytohormones moderate the adverse effects of salinity and improve growth. Understanding the complex hormonal crosstalk in cereals under salt stress will advance the knowledge about cooperation or antagonistic mechanisms among hormones and their role in developing salttolerant cereals to enhance the productivity of saline agricultural land. In this context, the present review focuses on the mechanisms of hormonal crosstalk that mediate the salt stress response and adaptation in graminaceous crops.

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Improved ATP synthesis and consumption in Triticum aestivum roots are involved in the nitrate-dependent alleviation of ammonium toxicity

Kong,

Liu,

Tang

et al. 2023

Plant Growth Regul

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Copalyl Diphosphate Synthase Mutation Improved Salt Tolerance in Maize (Zea mays. L) via Enhancing Vacuolar Na+ Sequestration and Maintaining ROS Homeostasis

Cited by 13 publications

References 63 publications

Glycine betaine increases salt tolerance in maize (Zea mays L.) by regulating Na+ homeostasis

Glycine betaine increases salt tolerance in maize (Zea mays L.) by regulating Na+ homeostasis

Hormonal crosstalk in regulating salinity stress tolerance in graminaceous crops

Improved ATP synthesis and consumption in Triticum aestivum roots are involved in the nitrate-dependent alleviation of ammonium toxicity

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