COPB2: a transport protein with multifaceted roles in cancer development and progression

Feng, Yanyan; Lei, X. L.; Zhang, L; Wan, Hongyuan; Pan, Hudan; Wu, Junqiang; Zou, Ming-Li; Zhu, Ling; Mi, Yuanyuan

doi:10.1007/s12094-021-02630-9

Cited by 15 publications

(14 citation statements)

References 64 publications

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“…After so many researches, the differential and functional COPB2 protein was identified. COPB2 is involved in material transport, energy metabolism as a vesicular envelope protein and tumor progression (15,(26)(27)(28).…”

Section: Discussionmentioning

confidence: 99%

“…To identify potential biomarkers or drug targets for PCa/ CRPC, the differential expression of COPB2 was detected by label-free quantitative proteomics (13). COPB2 is situated on chromosome 3q23 and encodes a protein containing 906 amino acids (102.5 kDa) (14,15). Furthermore, COPB2 is not only involved in vesicle transport, but also participates in the development of tumor cells as an oncogene (15).…”

Section: Introductionmentioning

confidence: 99%

“…COPB2 is situated on chromosome 3q23 and encodes a protein containing 906 amino acids (102.5 kDa) (14,15). Furthermore, COPB2 is not only involved in vesicle transport, but also participates in the development of tumor cells as an oncogene (15). COPB2 was also proved to be involved in PCa cell proliferation, cycle, and apoptosis in vitro (13,16).…”

Section: Introductionmentioning

confidence: 99%

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Upregulation of COPB2 Promotes Prostate Cancer Proliferation and Invasion Through the MAPK/TGF-β Signaling Pathway

et al. 2022

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There is increasing evidence that coatomer protein complex subunit beta 2 (COPB2) plays an important role in various cancer types. This study explored the role and the downstream mediators of COPB2 in prostate cancer (PCa). The expression of COPB2 was determined by the Cancer Genome Atlas database and enzyme-linked immunosorbent assay. COPB2 expression was upregulated in PCa tissues and correlated with Gleason score, biochemical recurrence, and poor prognosis. The functional roles of COPB2 in PCa were verified through a series of experiments. Knocking down COPB2 expression inhibited the growth and clonogenesis of PCa cells, promoted cell apoptosis, and inhibited the ability of scratch repair, invasion of PCa cells, and tumor growth in Nude mice. To analyze downstream signaling pathways, ingenuity pathway analysis, GSEA, and whole-genome expression spectrum GeneChip analysis were used. Western blot revealed that COPB2 expression promoted the proliferation and invasion of PCa cells by regulating the MAPK/TGF-β signaling pathway. The interacting protein (nuclear protein 1, NUPR1) was identified via Co-IP, real-time PCR, Western blot, and TCGA database in sampled tissues. The expressions of the interaction proteins NUPR1 and COPB2 were negatively regulated by each other. COPB2 could be a new biomarker for PCa diagnosis and monitoring and to provide a theoretical basis for identifying effective drug intervention targets through in-depth mechanistic studies.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Upregulation of COPB2 Promotes Prostate Cancer Proliferation and Invasion Through the MAPK/TGF-β Signaling Pathway

et al. 2022

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“…Furthermore, the range of individual patient responses to infectious diseases makes it difficult to identify appropriate treatment regimens. Recent work identified blood-based biomarkers as predictors of COVID-19 prognosis, where an increase in COPB2, a protein responsible for vesicle transport, was associated with milder symptoms and swifter recovery 48 , 49 . Although this study did not specifically investigate EVs, it motivates future work to understand EVs and cargoes as biomarkers of disease severity, where the sensitivity of diagnostic assays may be improved using EVs and mRNA cargoes.…”

Section: Mrna Cargoes As Biomarkers Of Diseasementioning

confidence: 99%

Extracellular vesicles as carriers of mRNA: Opportunities and challenges in diagnosis and treatment

Kirian,

Steinman,

Jewell

et al. 2024

Theranostics

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Extracellular vesicles (EVs) are produced by all cells in the body. These biological nanoparticles facilitate cellular communication through the transport of diverse cargoes, including small molecules, proteins, and nucleic acids. mRNA cargoes have gained particular interest given their role in the translation of functional proteins. As a biomarker platform, EVs can be found in nearly all biofluids—blood, mucus, urine, cerebrospinal fluid, and saliva—providing real-time insight into parent cell and tissue function. mRNAs carried by EVs are protected from degradation, resulting in improved detection compared to free mRNA, and recent work demonstrates promising results in using these mRNA cargoes as biomarkers for cancer, neurological diseases, infectious diseases, and gynecologic and obstetric outcomes. Furthermore, given the innate cargo carrying, targeting, and barrier crossing abilities of EVs, these structures have been proposed as therapeutic carriers of mRNA. Recent advances demonstrate methods for loading mRNAs into EVs for a range of disease indications. Here, we review recent studies using EVs and their mRNA cargoes as diagnostics and therapeutics. We discuss challenges associated with EVs in diagnostic and therapeutic applications and highlight opportunities for future development.

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“…To confirm the effect of gene silencing, the authors performed rescue experiments expressing siRNA-resistant gene constructs and demonstrated that sporozoite growth was restored in the complemented cell lines. COPB2 and COPG1 were visualized surrounding the parasitophorous vacuole and belong to the coatomer protein complex, which is responsible for the transport of vesicles between the Golgi apparatus and the endoplasmic reticulum ( Feng et al., 2021 ). Moreover, members of the GGA1 family are vesicle coating proteins involved in regulating protein traffic between the trans-Golgi network and the lysosome ( Bonifacino, 2004 ).…”

Section: Introductionmentioning

confidence: 99%

Forward Genetics in Apicomplexa Biology: The Host Side of the Story

Sánchez‐Arcila

Jensen

2022

Front. Cell. Infect. Microbiol.

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Forward genetic approaches have been widely used in parasitology and have proven their power to reveal the complexities of host-parasite interactions in an unbiased fashion. Many aspects of the parasite’s biology, including the identification of virulence factors, replication determinants, antibiotic resistance genes, and other factors required for parasitic life, have been discovered using such strategies. Forward genetic approaches have also been employed to understand host resistance mechanisms to parasitic infection. Here, we will introduce and review all forward genetic approaches that have been used to identify host factors involved with Apicomplexa infections, which include classical genetic screens and QTL mapping, GWAS, ENU mutagenesis, overexpression, RNAi and CRISPR-Cas9 library screens. Collectively, these screens have improved our understanding of host resistance mechanisms, immune regulation, vaccine and drug designs for Apicomplexa parasites. We will also discuss how recent advances in molecular genetics give present opportunities to further explore host-parasite relationships.

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COPB2: a transport protein with multifaceted roles in cancer development and progression

Cited by 15 publications

References 64 publications

Upregulation of COPB2 Promotes Prostate Cancer Proliferation and Invasion Through the MAPK/TGF-β Signaling Pathway

Upregulation of COPB2 Promotes Prostate Cancer Proliferation and Invasion Through the MAPK/TGF-β Signaling Pathway

Extracellular vesicles as carriers of mRNA: Opportunities and challenges in diagnosis and treatment

Forward Genetics in Apicomplexa Biology: The Host Side of the Story

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