COPII Components Sar1b and Sar1c Play Distinct Yet Interchangeable Roles in Pollen Development

Xin, Liang; Li, Shan-Wei; Gong, Limin

doi:10.1104/pp.20.00159

Cited by 29 publications

(22 citation statements)

References 53 publications

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“…Arabidopsis SAR1B and SAR1C also have interchangeable roles in microspore development (Liang et al ., 2020). SAR1B shows high expression in the tapetum, where it plays an important role in pollen development.…”

Section: Discussionmentioning

confidence: 99%

“…SAR1C is weakly expressed in the tapetum, yet sar1c exhibits normal pollen development. Further, SAR1C expression driven by the tapetal cell‐specific promoter A9 rescued the sar1b mutant phenotype (Liang et al ., 2020). Zebrafish and murine SEC23A and SEC23B are also interchangeable in vivo (Khoriaty et al ., 2018).…”

Section: Discussionmentioning

confidence: 99%

“…Among Sar1A–C, Sar1B may play a vital cellular role because only the sar1b mutant exhibits pollen infertility. Nevertheless, Sar1B and Sar1C have interchangeable roles in pollen development (Liang et al ., 2020). The sec23asec23d mutant also showed decreased fertility due to impaired pollen and tapetum development, which was more pronounced than that in sec23a or sec23d (Aboulela et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

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COPII genes SEC31A/B are essential for gametogenesis and interchangeable in pollen development in Arabidopsis

et al. 2021

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In eukaryotes, coat protein complex II (COPII) vesicles mediate anterograde traffic from the endoplasmic reticulum to the Golgi apparatus. Compared to yeasts, plants have multiple COPII coat proteins; however, the functional diversity among them is less well understood. SEC31A and SEC31B are outer coat proteins found in COPII vesicles in Arabidopsis. In this study, we explored the function of SEC31A and compared it with that of SEC31B from various perspectives. SEC31A was widely expressed, but at a significantly lower level than SEC31B. SEC31A-mCherry and SEC31B-GFP exhibited a high co-localization rate in pollen, but a lower rate in growing pollen tubes. The sec31a single mutant exhibited normal growth. SEC31A expression driven by the SEC31B promoter rescued the pollen abortion and infertility observed in sec31b. A sec31a-sec31b double mutant was unavailable due to lethality of the sec31asec31b gametophyte. Transmission electron microscopy revealed that one quarter of male gametogenesis was arrested at the uninuclear microspore stage, while confocal laser scanning microscopy showed that 1/4 female gametophyte development was suspended at the functional megaspore stage in sec31a-1/+sec31b-3/+ plants. Our study highlights the essential role of SEC31A/B in gametogenesis and their interchangeable functions in pollen development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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COPII genes SEC31A/B are essential for gametogenesis and interchangeable in pollen development in Arabidopsis

et al. 2021

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“…Its mutations resulted in defective gametophytic development at similar stages to those of asnap [ 49 ]. A few other mutants in which vacuolar trafficking was compromised also showed defective gametophytic development, such as the mutants of AP-1μ / HAPLESS13 [ 13 ], the mutants of PI(3,5)P 2 -metabolizing enzymes [ 14 , 15 ], as well as the mutants of COPII complexes [ 50 , 51 ].…”

Section: Discussionmentioning

confidence: 99%

The canonical α-SNAP is essential for gametophytic development in Arabidopsis

Liu¹,

Li²,

Li³

et al. 2021

PLoS Genet

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The development of male and female gametophytes is a pre-requisite for successful reproduction of angiosperms. Factors mediating vesicular trafficking are among the key regulators controlling gametophytic development. Fusion between vesicles and target membranes requires the assembly of a fusogenic soluble N-ethylmaleimide sensitive factor attachment protein receptors (SNAREs) complex, whose disassembly in turn ensures the recycle of individual SNARE components. The disassembly of post-fusion SNARE complexes is controlled by the AAA+ ATPase N-ethylmaleimide-sensitive factor (Sec18/NSF) and soluble NSF attachment protein (Sec17/α-SNAP) in yeast and metazoans. Although non-canonical α-SNAPs have been functionally characterized in soybeans, the biological function of canonical α-SNAPs has yet to be demonstrated in plants. We report here that the canonical α-SNAP in Arabidopsis is essential for male and female gametophytic development. Functional loss of the canonical α-SNAP in Arabidopsis results in gametophytic lethality by arresting the first mitosis during gametogenesis. We further show that Arabidopsis α-SNAP encodes two isoforms due to alternative splicing. Both isoforms interact with the Arabidopsis homolog of NSF whereas have distinct subcellular localizations. The presence of similar alternative splicing of human α-SNAP indicates that functional distinction of two α-SNAP isoforms is evolutionarily conserved.

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“…Rice hexokinase hxk5 gene mutant created by CRISPR/Cas9 results in male sterility on rice [110] . Knockout of COPII components sarib and saric genes also altered pollen development and caused male sterility in Arabidopsis [111] . Bnspl3 mutants, generated by CRISPR/Cas9 technology, exhibited developmental delay phenotype in allotetraploid oilseed rape [112] .…”

Section: Application Of Crispr/cas On Crop Improvementmentioning

confidence: 99%

CRISPR/Cas: A powerful tool for gene function study and crop improvement

Zhang

Ünver³

et al. 2021

Journal of Advanced Research

209

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COPII Components Sar1b and Sar1c Play Distinct Yet Interchangeable Roles in Pollen Development

Abstract: The small GTPase Sar1, components of the COPII complex that mediates vesicular trafficking from ER to Golgi, participate in sporophytic and gametophytic control of pollen development in Arabidopsis, with different isoforms playing distinct or redundant roles.

Cited by 29 publications

References 53 publications

COPII genes SEC31A/B are essential for gametogenesis and interchangeable in pollen development in Arabidopsis

COPII genes SEC31A/B are essential for gametogenesis and interchangeable in pollen development in Arabidopsis

The canonical α-SNAP is essential for gametophytic development in Arabidopsis

CRISPR/Cas: A powerful tool for gene function study and crop improvement

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