Copper–adenine complex, a compound, with multi-biochemical targets and potential anti-cancer effect

Hammud, Hassan H.; Nemer, Georges; Walid, Sawma; Touma, Jhonny; Barnabe, Pascale; Yolla, Bou-Mouglabey; Ghannoum, Amer M.; El-Hajjar, Jida; Usta, Jinan

doi:10.1016/j.cbi.2008.03.005

Cited by 47 publications

(23 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…17 Adenine complexes also have an anticancer effect. 18 Adenosine can act as an anticancer agent in lymphoma. 19 The anti-tumor promoting effect of glycyrrhetinic acid has been reported.…”

Section: Discussionmentioning

confidence: 99%

Regulation of p21, MMP-1, and MDR-1 Expression in Human Colon Carcinoma HT29 Cells by Tian Xian Liquid, a Chinese Medicinal Formula, In Vitro and In Vivo

et al. 2010

View full text Add to dashboard Cite

Ethnopharmacological relevance. Tian-Xian liquid (TXL), a commercially available Chinese medicine decoction, has been used as an anticancer dietary agent for more than 10 years without reported side effects. Aim of the study. The safety and quality consistency of TXL and its mechanisms of action on antiproliferation, antimetastasis, and reversion of multidrug resistance (MDR) regimens were explored. Materials and methods. In this study, an atomic absorption spectrophotometer and reversed phase high performance liquid chromatography with photodiode array detection (HPLC-DAD) were used to evaluate the main toxic elements and the quality consistency among different batches of TXL extracts, respectively. HT29 human colon cancer cell line and tumor-bearing nude mice were used. TXL was provided by China-Japan Feida Union Company Limited. The effect of TXL on in vitro proliferation of HT29 human colon cancer cell line was examined. The percentages of treated cells distributed in different phases of the cell cycles were analyzed by flow cytometry. Antiproliferative effect after treatment with TXL was assessed by determination of the protein levels of p21, cyclinD1, PCNA, and cdk-2, which are the key regulators for cell cycle progression. Meanwhile, the protein levels of MMP-1 and MDR-1 (multidrug resistance protein-1) were also determined to assess the effect of TXL on antimetastasis and reversion of MDR regimen, respectively. Results. The contents of main toxic elements were lower in TXL extract compared with the standard set by the Department of Health of the Government of Hong Kong Special Administrative Region (SAR). Our HPLC results showed that the relative standard deviations of the amount of the 5 standards were less than 5% in different batches of TXL. Immunoblotting analysis revealed a dramatic induction of cyclin kinase inhibitor p21 as well as an inhibition of cyclinD1, PCNA, and cdk-2 in the TXL-treated in vitro models, thereby, impeding cell progression from G1/S phase. Results obtained from the in vivo study also demonstrated that TXL upregulated the protein level of p21 and downregulated the protein levels of MMP-1 and MDR-1. Conclusions. Results obtained from the present investigation not only demonstrate the safety and quality of TXL extract but also demonstrate that TXL possesses antiproliferative and antimetastatic activities and brings about reversion of MDR on HT29 cell and on xenografted tissue in tumor-implanted nude mice.

show abstract

“…17 Adenine complexes also have an anticancer effect. 18 Adenosine can act as an anticancer agent in lymphoma. 19 The anti-tumor promoting effect of glycyrrhetinic acid has been reported.…”

Section: Discussionmentioning

confidence: 99%

Regulation of p21, MMP-1, and MDR-1 Expression in Human Colon Carcinoma HT29 Cells by Tian Xian Liquid, a Chinese Medicinal Formula, In Vitro and In Vivo

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Alkaline phosphatase cofactor; antioxidant and superoxide dismutase (SOD) cofactor (730,731) ; skeletal growth and maturation, and bone metabolism (732) ; chemical inactivator (46) ; formation of active carcinogenic compounds (93) ; Zn-binding compounds and cancer cell death (733) ; oesophagus cancer (734) ; Zn sensing receptor and cell signalling (735) ; immune functions (736) ; inflammatory diseases and cell signalling mechanisms (737) ; type 2 diabetes (738) ; food intake (739) Mn (569,700) : Antioxidant (740) ; metalloenzyme constituent and enzyme activation (569) ; bone health (732,741) ; manganese-SOD, NFkB activation and carcinogenic process (742) ; manganese-SOD and tumour growth (743) Cu (204,700) : Antioxidant (744) ; Cu-containing/binding proteins (569) ; bone health (732,745) ; central nervous system dysfunction (700) ; immune and cardiac dysfunctions (700,746,747) ; heart health (748,749) ; anti-cancer effect and DNA binding (750) ; risk of CHD (751,752) Se (204) :…”

Section: Appendixmentioning

confidence: 99%

New hypotheses for the health-protective mechanisms of whole-grain cereals: what is beyond fibre?

2010

View full text Add to dashboard Cite

Epidemiological studies have clearly shown that whole-grain cereals can protect against obesity, diabetes, CVD and cancers. The specific effects of food structure (increased satiety, reduced transit time and glycaemic response), fibre (improved faecal bulking and satiety, viscosity and SCFA production, and/or reduced glycaemic response) and Mg (better glycaemic homeostasis through increased insulin secretion), together with the antioxidant and anti-carcinogenic properties of numerous bioactive compounds, especially those in the bran and germ (minerals, trace elements, vitamins, carotenoids, polyphenols and alkylresorcinols), are today well-recognised mechanisms in this protection. Recent findings, the exhaustive listing of bioactive compounds found in whole-grain wheat, their content in whole-grain, bran and germ fractions and their estimated bioavailability, have led to new hypotheses. The involvement of polyphenols in cell signalling and gene regulation, and of sulfur compounds, lignin and phytic acid should be considered in antioxidant protection. Whole-grain wheat is also a rich source of methyl donors and lipotropes (methionine, betaine, choline, inositol and folates) that may be involved in cardiovascular and/or hepatic protection, lipid metabolism and DNA methylation. Potential protective effects of bound phenolic acids within the colon, of the B-complex vitamins on the nervous system and mental health, of oligosaccharides as prebiotics, of compounds associated with skeleton health, and of other compounds such as a-linolenic acid, policosanol, melatonin, phytosterols and para-aminobenzoic acid also deserve to be studied in more depth. Finally, benefits of nutrigenomics to study complex physiological effects of the 'whole-grain package', and the most promising ways for improving the nutritional quality of cereal products are discussed. Whole-grain wheat: Bioactive compounds: Physiological mechanisms: Health IntroductionThere is growing evidence that whole-grain cereal products protect against the development of chronic diseases. The most important of these in terms of public health are obesity (1,2) , the metabolic syndrome (3,4) , type 2 diabetes (5,6) , CVD (7) and cancers (8 -12) . Whole-grain cereal consumption has also been shown to be protective against mortality, as was shown with inflammation-related death (i.e. noncardiovascular and non-cancer inflammatory diseases such as, for example, respiratory system diseases) (13) and with cancer and CVD (4,14,15) . These conclusions are supported by the effects of consuming refined cereal products (bread, pasta and rice), as these have been associated with an increased risk of digestive tract, pharynx, larynx and thyroid cancers in northern Italians (16) . However, an association between a lower risk of developing a chronic disease and a high whole-grain cereal consumption does not mean a direct causal relationship and provides no information about the physiological mechanisms involved.These metabolic diseases are related to our daily lifestyle, no...

show abstract

“…Metal coordination complexes are used as enzyme inhibitors, antibacterials, antiviral and anticarcinogens in clinic applications [1][2][3][4][5][6][7][8][9]. Platinum, gold, vanadium, iron, molybdenum, cobalt, zinc, gallium, copper and many other metals are used in these complexes [8].…”

Section: Introductionmentioning

confidence: 99%

“…Adenine is also present in living cells as A-nucleotides adenosine monophosphate (AMP), adenosine diphosphate (ADP) and adenosine triphosphate (ATP) that play a vital role in many metabolic processes. Adenine is capable of forming chelates and its anticancer property increases when it is bound to metals [9,[15][16][17][18]. Most of the biochemical processes in living organisms are in the solution phase and in this phase trace amount of metal ions are available.…”

Section: Introductionmentioning

confidence: 99%

Equilibrium Study of the Mixed Complexes of Copper(II) with Adenine and Amino Acids in Aqueous Solution

Türkel

2015

J Solution Chem

View full text Add to dashboard Cite

In the present study, the protonation constants of adenine (Ade), a DNA base, and of the amino acids aspartic acid, glutamic acid, asparagine, leucine, phenylalanine and tryptophan, were determined in 0.1 molÁL -1 KNO 3 ionic medium and at 25.00°C by the potentiometric method. The binary and ternary ligand complex systems that Cu(II) establishes with Ade and these amino acids were examined using the same method and in the same ionic medium; stability constants were calculated with the BEST computer software and distribution curves were drawn by means of the SPE software. The relative stability of each ternary complex was compared with that of the corresponding binary complexes in terms of their Dlog 10 K values.

show abstract

Copper–adenine complex, a compound, with multi-biochemical targets and potential anti-cancer effect

Cited by 47 publications

References 46 publications

Regulation of p21, MMP-1, and MDR-1 Expression in Human Colon Carcinoma HT29 Cells by Tian Xian Liquid, a Chinese Medicinal Formula, In Vitro and In Vivo

Regulation of p21, MMP-1, and MDR-1 Expression in Human Colon Carcinoma HT29 Cells by Tian Xian Liquid, a Chinese Medicinal Formula, In Vitro and In Vivo

New hypotheses for the health-protective mechanisms of whole-grain cereals: what is beyond fibre?

Equilibrium Study of the Mixed Complexes of Copper(II) with Adenine and Amino Acids in Aqueous Solution

Contact Info

Product

Resources

About