Copper containing wood preservatives shifted bacterial and fungal community compositions in pine sapwood in two field sites

Noll, Matthias; Buettner, Christian; Lasota, Sandra

doi:10.1016/j.ibiod.2019.04.007

Cited by 13 publications

(8 citation statements)

References 51 publications

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“…There, both Cu and CuTriQAC wood preservatives shifted bacterial and fungal community compositions in buried wood specimens. In addition, Cu‐ and CuTriQAC‐treated wood selected for ascomycetes fungi and bacterial diazotrophs that fix atmospheric nitrogen (Noll et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

“…Likewise, in terrestrial habitats, researchers have also shown impacts on microbial communities (bacteria and fungi) from Cu exposures (Bååth et al, 1998; Chao‐Rong & Zhang, 2011; Macdonald et al, 2011; Ranjard et al, 2006). Although some recent studies have dealt with microbiome effects of Cu released from older wood preservative formulations (Kirker et al, 2017; Lasota et al, 2019; Noll et al, 2019), there is little information on microbial changes associated with MCA‐treated wood.…”

Section: Introductionmentioning

confidence: 99%

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Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Originating from Micronized Copper Azole–Treated Wood

Reichman

Johnson

Rygiewicz

et al. 2021

Enviro Toxic and Chemistry

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Micronized copper (Cu) azole (MCA) wood preservative formulations include Cu in nano form, and relatively little is known about longer term effects of Cu leached from MCA into wetland ecosystems. We tested the hypothesis that changes in soil microbiomes within reconstructed freshwater wetlands will be associated with exposure to elevated Cu concentrations originating from immersed MCA-treated wood stakes. Eight replicate communities were assembled with Willamette Valley (OR, USA) flood plain soil and clonally propagated wetland plants within mesocosms. Inundated communities were equilibrated for 5 months before installation of MCA or control southern yellow pine stakes (n = 4 communities/experimental group). Soil samples were collected for 16S and internal transcribed spacer amplicon sequencing to quantify responses in prokaryotes and eukaryotes, respectively, at 15 time points, spanning two simulated seasonal dry downs, for up to 678 days. Physiochemical properties of water and soil were monitored at 20 and 12 time points respectively, over the same period. For both taxonomic groups of organisms, phylogenetic diversity increased and was positively correlated with elapsed days. Furthermore, there was significant divergence among eukaryotes during the second year based on experimental group. Although the composition of taxa underwent succession over time, there was significantly reduced relative abundance of sequence variants from Gomphonema diatoms and Scutellinia fungi in communities where MCA wood stakes were present compared with the controls. These focused microbiome shifts were positively correlated with surface water Cu and soil Cu concentrations, which were significantly elevated in treated communities. The reconstructed communities were effective systems for assessing potential impacts to wetland microbiomes after exposure to released copper. The results further inform postcommercialization risk assessments on MCA-treated wood.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Originating from Micronized Copper Azole–Treated Wood

Reichman

Johnson

Rygiewicz

et al. 2021

Enviro Toxic and Chemistry

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“…could shift the bacterial community composition (figure 1), which leads to decreased deadwood decay rates. Deadwood has a high bacterial biomass (2 × 10 12 gene copy numbers g −1 ) [43,44], which is more than enough to enable predatory lifestyle of Bdellovibrio spp. with need of a minimal concentration of 7 × 10 5 prey cells ml −1 [45,46].…”

Section: Discussionmentioning

confidence: 99%

Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay

et al. 2022

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Microbial community members are the primary microbial colonizers and active decomposers of deadwood. This study placed sterilized standardized beech and spruce sapwood specimens on the forest ground of 8 beech- and 8 spruce-dominated forest sites. After 370 days, specimens were assessed for mass loss, nitrogen (N) content and 15 N isotopic signature, hydrolytic and lignin-modifying enzyme activities. Each specimen was incubated with bromodeoxyuridine (BrdU) to label metabolically active fungal and bacterial community members, which were assessed using amplicon sequencing. Fungal saprotrophs colonized the deadwood accompanied by a distinct bacterial community that was capable of cellulose degradation, aromatic depolymerization, and N 2 fixation. The latter were governed by the genus Sphingomonas , which was co-present with the majority of saprotrophic fungi regardless of whether beech or spruce specimens were decayed. Moreover, the richness of the diazotrophic Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium group was significantly correlated with mass loss, N content and 15 N isotopic signature. By contrast, presence of obligate predator Bdellovibrio spp. shifted bacterial community composition and were linked to decreased beech deadwood decay rates. Our study provides the first account of the composition and function of metabolically active wood-colonizing bacterial and fungal communities, highlighting cross-kingdom interactions during the early and intermediate stages of wood decay.

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“…The diversity and quantity of TBPs have been assessed by a variety of molecular methodologies, including next-generation sequencing (NGS) such as amplicon sequencing [1]. NGS is a quick and cost-effective technology that enhanced our knowledge of genes and genomes of single cells and compositions of complex microbial communities in the last decade [1,6]. The 16S ribosomal RNA (rRNA) and its gene have been frequently used as a target for amplicon sequencing to identify bacterial taxa [1,6,7].…”

Section: Introductionmentioning

confidence: 99%

“…NGS is a quick and cost-effective technology that enhanced our knowledge of genes and genomes of single cells and compositions of complex microbial communities in the last decade [1,6]. The 16S ribosomal RNA (rRNA) and its gene have been frequently used as a target for amplicon sequencing to identify bacterial taxa [1,6,7]. Therefore, nine hypervariable regions (V1-V9) in the rRNA gene have been assessed of which regions V1-V4 are most commonly used to explore the bacterial sequence diversity in ticks [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Tick Surface Decontamination Methods

Hoffmann

Fingerle²,

Noll

2020

Microorganisms

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Various microbial pathogens have been found in ticks such as Ixodes ricinus. However, most studies assessed tick microbiomes without prior decontamination of the tick surface, which may alter the results and mislead conclusions regarding the composition of the tick-borne microbiome. The aim of this study was to test four different decontamination methods, namely (i.) 70% ethanol, (ii.) DNA Away, (iii.) 5% sodium hypochlorite and (iv.) Reactive Skin Decontamination Lotion (RSDL), which have been previously reported for tick surface and animal or human skin decontamination. To test the efficiency of decontamination, we contaminated each tick with a defined mixture of Escherichia coli, Micrococcus luteus, Pseudomonas fluorescens, dog saliva and human sweat. No contamination was used as a negative control, and for a positive control, a no decontamination strategy was carried out. After nucleic acid extraction, the recovery rate of contaminants was determined for RNA and DNA samples by qPCR and tick-borne microbiome analyses by bacterial 16S rRNA and 16S rRNA gene amplicon sequencing. Ticks treated with 5% sodium hypochlorite revealed the lowest number of contaminants followed by DNA Away, RSDL and 70% ethanol. Moreover, tick microbiomes after 5% sodium hypochlorite decontamination clustered with negative controls. Therefore, the efficiency of decontamination was optimal with 5% sodium hypochlorite and is recommended for upcoming studies to address the unbiased detection of tick-borne pathogens.

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Copper containing wood preservatives shifted bacterial and fungal community compositions in pine sapwood in two field sites

Cited by 13 publications

References 51 publications

Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Originating from Micronized Copper Azole–Treated Wood

Focused Microbiome Shifts in Reconstructed Wetlands Correlated with Elevated Copper Concentrations Originating from Micronized Copper Azole–Treated Wood

Cross-kingdom interactions and functional patterns of active microbiota matter in governing deadwood decay

Analysis of Tick Surface Decontamination Methods

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