Copper deposition-induced efficient signal amplification for ultrasensitive lateral flow immunoassay

Tian, Mi; Lei, Lulu; Xie, Wenyue; Yang, Qimeng; Li, Chang Ming; Liu, Yingshuai

doi:10.1016/j.snb.2018.11.028

Cited by 38 publications

(22 citation statements)

References 29 publications

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“…Lateral flow immunoassay (LFIA) has been considered one of the most popular point-of-care testing (POCT) techniques due to its simplicity, flexibility, speed, low cost, and wide adaptability [ [12] , [13] , [14] ]. The LFIA-based serological testing of anti-SARS-CoV-2 IgM/IgG is a good choice for the simple and effective diagnosis of COVID-19 because of the following advantages.…”

Section: Introductionmentioning

confidence: 99%

Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples

Liu

Dai

Xiao

et al. 2021

Sensors and Actuators B: Chemical

240

192

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Section: Introductionmentioning

confidence: 99%

Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples

Liu

Dai

Xiao

et al. 2021

Sensors and Actuators B: Chemical

240

192

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“…Li et al reported a copper deposition-assisted method to amplify the AuNP-LFS for the sensitive detection of human chorionic gonadotropin with the LOD of 1 pg mL −1 , with ca. two to three orders of magnitude improvement than that of conventional AuNP-LFS ( Tian et al, 2019 ). Nonetheless, in traditional MISG, the metal ions (i.e., Au, Ag, and Cu) are directly bound with protein or capping agents on the surface of AuNP probes and reduced to metal shell in the presence of reducing agents.…”

Section: Introductionmentioning

confidence: 93%

Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics

Zhou

Chen

Liu

et al. 2021

Biosensors and Bioelectronics

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A polyethyleneimine (PEI)-assisted copper in-situ growth (CISG) strategy was proposed as a controlled signal amplification strategy to enhance the sensitivity of gold nanoparticle-based lateral flow sensors (AuNP-LFS). The controlled signal amplification is achieved by introducing PEI as a structure-directing agent to regulate the thermodynamics of anisotropic Cu nanoshell growth on the AuNP surface, thus controlling shape and size of the resultant AuNP@Cu core–shell nanostructures and confining free reduction and self-nucleation of Cu 2+ for improved reproducibility and decreased false positives. The PEI-CISG-enhanced AuNP-LFS showed ultrahigh sensitivities with the detection limits of 50 fg mL −1 for HIV-1 capsid p24 antigen and 6 CFU mL −1 for Escherichia coli O157:H7. We further demonstrated its clinical diagnostic efficacy by configuring PEI-CISG into a commercial AuNP-LFS detection kit for SARS-CoV-2 antibody detection. Altogether, this work provides a reliable signal amplification platform to dramatically enhance the sensitivity of AuNP-LFS for rapid and accurate diagnostics of various infectious diseases.

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“…The gold nanoparticles (Au NPs) were prepared as previously described 46 . Briefly, sodium citrate solution (1%, w/v) was added into the boiling HAuCl 4 solution (0.01%, w/v).…”

Section: Fabrication Of Immunochromatographic Test Stripsmentioning

confidence: 99%

“…After stirring for 1 hour at 37°C, the bovine serum albumin (BSA) solution (0.1%, w/v) was added, and the Au NP-antibody conjugates were collected by centrifugation. The immunologic test strips were composed of a sample pad, a conjugate pad, an antibody pad, a NC membrane, and an adaptive backing card 46 . Briefly, the conjugate pad was dripped with appropriate amounts of Au NP-antibody conjugate suspension, and then dried for 1 hour at 37°C.…”

Section: Fabrication Of Immunochromatographic Test Stripsmentioning

confidence: 99%

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

Chen

et al. 2020

Cell Discov

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African swine fever virus (ASFV) is a dsDNA virus responsible for a severe, highly contagious, and lethal disease affecting both domestic and wild pigs. ASFV has brought enormous economic loss to a number of countries, and effective vaccine and therapy are still lacking. Therefore, a rapid, sensitive, and field-deployable detection of ASFV is important for disease surveillance and control. Herein, we developed a Cas12a-mediated portable paper assay to rapidly and precisely detect ASFV. We identified a robust set of crRNAs that recognized the highly conserved region of essential ASFV genes. The Cas12a-mediated detection assay showed low tolerance for mismatch mutations, and no cross-reactivity against other common swine pathogens. We further developed a paper-based assay to allow instrument-free detection of ASFV. Specifically, we applied gold nanoparticle-antibody conjugate to engineer homemade strips and combined it with Cas12a-mediated ASFV detection. This portable paper, instrument-free diagnostics, faithfully detected ASFV in swine samples, showing comparable sensitivity to the traditionally instrumentdependent qPCR method. Taking together, we developed a highly sensitive, instant, and economic Cas12a-mediated paper diagnostics of ASFV, with a great application potential for monitoring ASFV in the field.

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Copper deposition-induced efficient signal amplification for ultrasensitive lateral flow immunoassay

Cited by 38 publications

References 29 publications

Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples

Development of a SERS-based lateral flow immunoassay for rapid and ultra-sensitive detection of anti-SARS-CoV-2 IgM/IgG in clinical samples

Controlled copper in situ growth-amplified lateral flow sensors for sensitive, reliable, and field-deployable infectious disease diagnostics

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

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