Copper(II)-based metal affinity chromatography for the isolation of the anticancer agent bleomycin from Streptomyces verticillus culture

Gu, Jiesi; Codd, Rachel

doi:10.1016/j.jinorgbio.2012.01.015

Cited by 25 publications

(23 citation statements)

References 34 publications

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“…Bleomycin (BLM) is a group of glycopeptide antibiotics produced by Streptomyces verticillus. It is clinically used for the treatment of squamous cell carcinoma, malignant lymphoma, testicular cancer, cervical cancer, and other cancers [69]. Bleomycin causes DNA strand scission via the formation of an intermediate metal complex which requires a metal ion such as Cu(II) or Fe(III) [70,71].…”

Section: Bleomycinmentioning

confidence: 99%

Iron and Copper Intracellular Chelation as an Anticancer Drug Strategy

et al. 2018

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A very promising direction in the development of anticancer drugs is inhibiting the molecular pathways that keep cancer cells alive and able to metastasize. Copper and iron are two essential metals that play significant roles in the rapid proliferation of cancer cells and several chelators have been studied to suppress the bioavailability of these metals in the cells. This review discusses the major contributions that Cu and Fe play in the progression and spreading of cancer and evaluates select Cu and Fe chelators that demonstrate great promise as anticancer drugs. Efforts to improve the cellular delivery, efficacy, and tumor responsiveness of these chelators are also presented including a transmetallation strategy for dual targeting of Cu and Fe. To elucidate the effectiveness and specificity of Cu and Fe chelators for treating cancer, analytical tools are described for measuring Cu and Fe levels and for tracking the metals in cells, tissue, and the body.

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Section: Bleomycinmentioning

confidence: 99%

Iron and Copper Intracellular Chelation as an Anticancer Drug Strategy

et al. 2018

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“…As evident from these results, this simple pre-treatment increased the effective loading capacity for Cu 2+ -tacn-and Cu 2+ -dtnpSepharose™ 6 FF resins by at least 2-fold. These findings indicate that despite similarities in bulk Cu 2+ ion content for the three adsorbents, differences in binding capacities of the Cu 2+ -tacn-and Cu 2+ -dtnp-Sepharose™ 6 FF resins compared to the Cu 2+ -IMAC Sepharose™ 6 FF resin could be due to at least two effects, namely, surface chemistry differences between the tacn-related IMAC resins compared to the Cu 2+ -IMAC Sepharose™ 6 FF resin, resulting in different effective binding site θ occupancies [33,34]; and secondly due to different extents of binding of specific subsets of metal-binding metallophores or other nonspecific weak organic chelators, such as dicarboxylic acids, that are produced [37,38] [19][20]. In contrast, the Cu 2+ -tacn-and Cu 2+ -dtnp-Sepharose™ 6 FF adsorbents can be used for at least five cycles before the binding capacity fell below the threshold value of >50%, presumably due to the build-up of non-proteinaceous components made by the E. coli cells, stressed as a consequence of expression at higher levels of tagged heterologous proteins.…”

Section: Treatment Of E Coli Cells By Osmotic Shock Increases Proteimentioning

confidence: 99%

Changed loading conditions and lysate composition improve the purity of tagged recombinant proteins with tacn‐based IMAC adsorbents

Zhang

Fredericks

Longford

et al. 2014

Biotechnology Journal

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These investigations were designed to improve capture efficiency and selectivity in the immobilized metal ion affinity chromatographic (IMAC) purification of tagged recombinant proteins expressed in Escherichia coli cells, utilizing an alternative and novel class of immobilized metal binding ligands. The impact of loading conditions and lysate composition on the IMAC purification of NT1A- or His6 -tagged green fluorescent protein (GFP), using the ligands 1,4,7-triazacyclononane (tacn) and bis(1,4,7-triazacyclononyl)propane (dtnp), charged with Cu(2+) ions, has thus been explored. These findings were compared to the performance of a commercial adsorbent, IMAC Sepharose™ 6 FF, similarly charged with Cu(2+) ions. With the same loading, wash and elution protocols, the tacn- and dtnp-derived adsorbents showed higher selectivity in terms of removal of E. coli host cell proteins than the commercial adsorbent, while low molecular weight components in the crude lysate had a higher impact on the binding capacities of tacn- and dtnp-derived adsorbents. This effect of lysate composition could be reduced through osmotic shock treatment of the E. coli cells prior to lysis. Additionally, the protein-binding capacities of the tacn-based resins were enhanced by increasing their ligand densities. Because both the tacn- and the dtnp-derived IMAC adsorbents exhibit very high metal ion stability constants, under the chromatographic conditions examined, they could be used several times without re-charging with Cu(2+) ions. The results of these studies thus expand the general application scope of tacn-based IMAC resins for use in the capture and purification of tagged recombinant proteins.

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“…The solvent was removed and the products were collected, and dried in vacuo. m-CF 3 PIP and p-CF 3 PIP were prepared by a similar procedure with the compound CF 3…”

Section: Synthesis Of Phenanthroline-based Ligands: Cf3pip M-cf3pip mentioning

confidence: 99%

“…The development of transition metal complexes which are actually to be utilized in a wide range of biochemistry and medicine as well as therapeutic medication against tumor cells has achieved fantastic step forward in past decades [1]- [3]. Oxovanadium complexes as one group of transition metal compounds have aroused great interest among biochemists and pharmacologists owing to diverse biologic and pharmacological activities such as antiproliferative [4], antibacterial [5], biocatalytic oxidation [6] [7], insulin-enhancing effects [8], apoptosis-inducing activity [9] [10], potential capabilities as DNA structural probes and DNA dependent electron transfer likewise [11] [12].…”

Section: Introductionmentioning

confidence: 99%

Photocleavage Properties and DNA-Binding Studies of Oxovanadium Complexes Incorporating 2-(2-Hydroxybenzylideneamino) Isoindoline-1,3-Dione and Fluoro-Phenanthroline Derivatives

Ying¹,

Zeng²,

Lu³

et al. 2014

OJIC

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Three mononuclear oxovanadium complexes [VO(Hbid)(CF3PIP)] (1) (Hbid=(E)-2-(2-hydroxybenzylideneamino)isoindoline-1,3-dione, CF3PIP=2-(2-trifluoromethyl phenyl)imidazole[4,5-f][1,10] phenanthroline), [VO(Hbid)(m-CF3PIP)]; (2) (m-CF3PIP=2-(3-trifluoromethyl phenyl)imidazole [4, 5-f][1,10]phenanthroline) and [VO(Hbid)(p-CF3PIP)]; (3) (p-CF3PIP=2-(4-trifluoromethyl phenyl) imidazole[4,5-f][1,10]phenanthroline) have been synthesized and characterized by elemental analysis, IR, molar conductance, ES-MS and 1 H NMR. The DNA-binding properties of these complexes were studied by using UV-Vis absorption titration, fluorescence spectra, viscosity measurements and thermal denaturation studies. The results show that 1, 2 and 3 interact with calf thymus DNA (CT-DNA) by intercalation modes and the magnitude of their intrinsic binding constants (Kb values) follows the order: 2 < 1 < 3. Furthermore, their photocleavage properties with pBR322 plasmid DNA were investigated by agarose gel electrophoresis experiments. The DNA cleavage capacity of complex 3 is also stronger than that of 1 and 2.

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Copper(II)-based metal affinity chromatography for the isolation of the anticancer agent bleomycin from Streptomyces verticillus culture

Cited by 25 publications

References 34 publications

Iron and Copper Intracellular Chelation as an Anticancer Drug Strategy

Iron and Copper Intracellular Chelation as an Anticancer Drug Strategy

Changed loading conditions and lysate composition improve the purity of tagged recombinant proteins with tacn‐based IMAC adsorbents

Photocleavage Properties and DNA-Binding Studies of Oxovanadium Complexes Incorporating 2-(2-Hydroxybenzylideneamino) Isoindoline-1,3-Dione and Fluoro-Phenanthroline Derivatives

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