Copper oxide nanoparticles: In vitro and in vivo toxicity, mechanisms of action and factors influencing their toxicology

Sajjad, Humna; Sajjad, Anila; Haya, Rida Tul; Khan, Muhammad Mustajab; Zia, Muhammad

doi:10.1016/j.cbpc.2023.109682

Cited by 45 publications

(14 citation statements)

References 260 publications

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“…Due to high toxicity and low stability, e.g., oxidation or aggregation, however, the applications of Cu nanoparticle in medicine are still under debate. Cu nanoparticle can impair the cell membrane integrity or organelles functions by direct contact, or produce excessive ROS to indirectly damage biomacromolecules including lipids, proteins, or nucleic acids [ [55] , [56] , [57] , [58] ]. Therefore, how to expand the therapeutic window of Cu nanoparticle but avoid its detrimental accumulation in the cells remains a major challenge.…”

Section: Discussionmentioning

confidence: 99%

Cupric-polymeric nanoreactors integrate into copper metabolism to promote chronic diabetic wounds healing

Tang,

Tan,

Leng

et al. 2024

Materials Today Bio

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Section: Discussionmentioning

confidence: 99%

Cupric-polymeric nanoreactors integrate into copper metabolism to promote chronic diabetic wounds healing

Tang,

Tan,

Leng

et al. 2024

Materials Today Bio

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“…The negative surface charge and substantial molecular weight of Pdots present challenges for biological metabolism. Therefore, a thorough exploration of Pdots' absorption, distribution, metabolism, and excretion processes, particularly their selective distribution and physical transformations (such as dissolution, aggregation, or morphological changes), remains an imperative and complex issue [ [230] , [231] , [232] , [233] , [234] ]. This encompasses the toxicological effects and safety of nanoparticles, a crucial concern for researchers.…”

Section: Characterization and Spectral Properties Of Semiconducting P...mentioning

confidence: 99%

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Zhang,

Yu,

et al. 2024

Materials Today Bio

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“…There are studies in the literature that claim that nanoparticles of heavy metals or metals with variable valence have a pronounced antimicrobial effect [ 30 , 45 , 46 , 47 , 48 , 49 , 50 ]. At the same time, there are also works in which the authors did not obtain such an effect [ 51 , 52 , 53 , 54 , 55 ]. This contradiction is also noted in systematic reviews [ 51 ]; therefore, this allows us to state the absence of unambiguous conclusions about the presence of antimicrobial properties of nanoparticles whilst taking into account publication bias (data with identified effects are published more often, and in the absence of positive conclusions, works are not published; this can lead to fundamental erroneous system conclusions).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of Citrate-Coated Cerium Oxide Nanoparticles

Silina,

Ivanova,

Manturova

et al. 2024

Nanomaterials

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The purpose of this study was to investigate the antimicrobial activity of citrate-stabilized sols of cerium oxide nanoparticles at different concentrations via different microbiological methods and to compare the effect with the peroxidase activity of nanoceria for the subsequent development of a regeneration-stimulating medical and/or veterinary wound-healing product providing new types of antimicrobial action. The object of this study was cerium oxide nanoparticles synthesized from aqueous solutions of cerium (III) nitrate hexahydrate and citric acid (the size of the nanoparticles was 3–5 nm, and their aggregates were 60–130 nm). Nanoceria oxide sols with a wide range of concentrations (10−1–10−6 M) as well as powder (the dry substance) were used. Both bacterial and fungal strains (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Candida albicans, Aspergillus brasielensis) were used for the microbiological studies. The antimicrobial activity of nanoceria was investigated across a wide range of concentrations using three methods sequentially; the antimicrobial activity was studied by examining diffusion into agar, the serial dilution method was used to detect the minimum inhibitory and bactericidal concentrations, and, finally, gas chromatography with mass-selective detection was performed to study the inhibition of E. coli’s growth. To study the redox activity of different concentrations of nanocerium, we studied the intensity of chemiluminescence in the oxidation reaction of luminol in the presence of hydrogen peroxide. As a result of this study’s use of the agar diffusion and serial dilution methods followed by sowing, no significant evidence of antimicrobial activity was found. At the same time, in the current study of antimicrobial activity against E. coli strains using gas chromatography with mass spectrometry, the ability of nanoceria to significantly inhibit the growth and reproduction of microorganisms after 24 h and, in particular, after 48 h of incubation at a wide range of concentrations, 10−2–10−5 M (48–95% reduction in the number of microbes with a significant dose-dependent effect) was determined as the optimum concentration. A reliable redox activity of nanoceria coated with citrate was established, increasing in proportion to the concentration, confirming the oxidative mechanism of the action of nanoceria. Thus, nanoceria have a dose-dependent bacteriostatic effect, which is most pronounced at concentrations of 10−2–10−3 M. Unlike the effects of classical antiseptics, the effect was manifested from 2 days and increased during the observation. To study the antimicrobial activity of nanomaterials, it is advisable not to use classical qualitative and semi-quantitative methods; rather, the employment of more accurate quantitative methods is advised, in particular, gas chromatography–mass spectrometry, during several days of incubation.

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Copper oxide nanoparticles: In vitro and in vivo toxicity, mechanisms of action and factors influencing their toxicology

Cited by 45 publications

References 260 publications

Cupric-polymeric nanoreactors integrate into copper metabolism to promote chronic diabetic wounds healing

Cupric-polymeric nanoreactors integrate into copper metabolism to promote chronic diabetic wounds healing

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Antimicrobial Activity of Citrate-Coated Cerium Oxide Nanoparticles

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