Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity

Sanjay, M. K.; Madabushi, Srideshikan Sargur; Vanishree, V.; Usha, M.; Raj, Anshu; Gaddad, Subhaschandra M.; Shivannavar, Channappa T.

doi:10.1007/s12088-011-0074-9

Cited by 3 publications

(2 citation statements)

References 23 publications

(21 reference statements)

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“…In this sense, E.coli and Salmonella typhimurium lower the damage caused by superoxide and secondary products (ONOO − and H 2 O 2 ) by having one and two periplasmic CuZn‐SODs, respectively . The presence of the gene encoding the periplasmic SOD has been shown to correlate with virulence in different strains of both E.coli and Salmonella typhimurium . In the same way, Trypanosoma cruzi apparently also excrete a Fe‐SOD as a defense mechanism .…”

Section: Evasion From the Peroxynitrite‐dependent Pathogen Killingmentioning

confidence: 99%

Peroxynitrite, a potent macrophage‐derived oxidizing cytotoxin to combat invading pathogens

2013

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Macrophages are among the first cellular actors facing the invasion of microorganisms. These cells are able to internalize pathogens and destroy them by means of toxic mediators, many of which are produced enzymatically and have strong oxidizing capacity. Indeed, macrophages count on the NADPH oxidase complex activity, which is triggered during pathogen invasion and leads to the production of superoxide radical inside the phagosome. At the same time, the induction of nitric oxide synthase results in the production of nitric oxide in the cytosol which is able to readily diffuse to the phagocytic vacuole. Superoxide radical and nitric oxide react at diffusion controlled rates with each other inside the phagosome to yield peroxynitrite, a powerful oxidant capable to kill microorganisms. Peroxynitrite toxicity resides on oxidations and nitrations of biomolecules in the target cell. The central role of peroxynitrite as a key effector molecule in the control of infections has been proven in a wide number of models. However, some microorganisms and virulent strains adapt to survive inside the potentially hostile oxidizing microenvironment of the phagosome by either impeding peroxynitrite formation or rapidly detoxifying it once formed. In this context, the outcome of the infection process is a result of the interplay between the macrophage-derived oxidizing cytotoxins such as peroxynitrite and the antioxidant defense machinery of the invading pathogens.

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Section: Evasion From the Peroxynitrite‐dependent Pathogen Killingmentioning

confidence: 99%

Peroxynitrite, a potent macrophage‐derived oxidizing cytotoxin to combat invading pathogens

2013

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“…The second major class of SODs uses copper and zinc ions as catalytic metal (Cu-ZnSOD). Cu-ZnSODs encoded by sodC in bacteria are present in the periplasm of many Gram-negative bacteria, including Escherichia coli and Salmonella [16] , [19] – [22] . Ni-containing SODs encoded by sodN were only lately discovered and seem to be less common [23] .…”

Section: Introductionmentioning

confidence: 99%

Impact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics

et al. 2014

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Acinetobacter baumannii is a Gram-negative bacterium appearing as an opportunistic pathogen in hospital settings. Superoxide dismutase (SOD) contributes to virulence in several pathogenic bacteria by detoxifying reactive oxygen species released in the course of host defense reactions. However, the biological role of SODs in A. baumannii has not yet been elucidated. Here, we inactivated in A. baumannii ATCC 17978 gene A1S_2343, encoding a putative SOD of the Fe-Mn type by transposon insertion, resulting in mutant ATCC 17978 sod2343::Km. The mutation was also introduced in two naturally competent A. baumannii isolates by transformation with chromosomal DNA derived from mutant ATCC 17978 sod2343::Km. We demonstrate that inactivation of sod2343 leads to significant motility defects in all three A. baumannii strains. The mutant strains were more susceptible to oxidative stress compared to their parental strains. Susceptibility to colistin and tetracycline was increased in all mutant strains while susceptibility of the mutants to gentamicin, levofloxacin and imipenem was strain-dependent. In the Galleria mellonella infection model the mutant strains were significantly attenuated. In conclusion, sod2343 plays an important role in motility, resistance to oxidative stress, susceptibility to antibiotics and virulence in A. baumannii.

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Proteome analysis of outer membrane vesicles from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease

Wang,

Zeng,

et al. 2024

Journal of Invertebrate Pathology

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Copper, Zinc-Superoxide Dismutase from Clinically Isolated Escherichia coli: Cloning, Analysis of sodC and Its Possible Role in Pathogenicity

Cited by 3 publications

References 23 publications

Peroxynitrite, a potent macrophage‐derived oxidizing cytotoxin to combat invading pathogens

Peroxynitrite, a potent macrophage‐derived oxidizing cytotoxin to combat invading pathogens

Impact of Acinetobacter baumannii Superoxide Dismutase on Motility, Virulence, Oxidative Stress Resistance and Susceptibility to Antibiotics

Proteome analysis of outer membrane vesicles from Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease

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