Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay

Malgor, Ramiro; Nonaka, Nariaki; Basmadjian, Isabel; Sakai, Heisuke; Carámbula, B.; Oku, Yuzaburo; Carmona, Carlos; Kamiya, Mitsunobu

doi:10.1016/s0020-7519(97)00127-6

Cited by 58 publications

(31 citation statements)

References 5 publications

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“…Some sandwich ELISA systems have been assessed for their ability to detect E. granulosus copro-antigens using monoclonal antibody produced against somatic extract of Echinococcus multilocularis. Although the test was very sensitive (100%) in naturally and experimentally infected animals, there were cases of cross-reactivity with Taenia hydatigena Malgor et al, 1997).…”

Section: Diagnosis Of E Granulosus In Dogsmentioning

confidence: 96%

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

Mohamed¹,

Wafa²,

Kawther³

et al. 2017

J. Vet. Med. Anim. Health

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Echinococcus granulosus is a tiny tapeworm that parasitizes the small intestine of canids, mainly dogs, which act as definitive hosts for the parasite. Infected dogs are the main source of infection to humans and livestock which act as intermediate hosts resulting in hydatid disease condition. E. granulosus is widely distributed in many parts of the world, and is very common in North African countries. In Libya, the rate of infection with echinococcosis in dogs was reported to be lower than 7 to 80% in stray dogs, 34.8 to 60% in sheep/guard dogs and 7.7 to 21.6% in farm/house dogs. This data fulfills the world health organization (WHO) criteria and suggests that the incidence of infection with echinococcosis/ hydatidosis in some parts of the country can be reaching the level of hyper endemic. Diagnosis of echinococcosis in infected dogs can be performed by isolating the parasite from their faeces or from the contents of their small intestine after necropsy. Recent developments in immunodiagnostic assays for echinococcosis in dogs have been described. Public health and risk factors as well as ways of hydatid disease treatment and various control strategies, including the use of veterinary vaccines, have also been discussed.

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Section: Diagnosis Of E Granulosus In Dogsmentioning

confidence: 96%

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

Mohamed¹,

Wafa²,

Kawther³

et al. 2017

J. Vet. Med. Anim. Health

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“…Use of the coproantigen ELISA test would be of great value in determination of the risk of canine echinococcosis in endemic areas (Lopera et al, 2003). They have successfully been used in Libya (Buishi et al, 2005), Peru (Lopera et al, 2003), Cyprus (Christofi et al, 2002) and Uruguay (Malgor et al, 1997) demonstrating their usefulness for epidemiological studies.…”

Section: Discussionmentioning

confidence: 99%

Detection of Echinococcus granulosus coproantigens in dogs from Antakya Province, Turkey

et al. 2008

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Seventy-nine owned dogs (47 males and 32 females) from Antakya province were examined for E. granulosus by coproantigen ELISA (CHEKIT®-ECHINOTEST) between April to July in 2006. The estimated prevalence of E. granulosus infection was 8.86 % (7/79). Mean age of the dogs with E. granulosus was 2.3 years ranging from one to five years. There was no statistically significant difference regarding the prevalence between male and female dogs. A significant difference was detected between unrestrained (or free roaming) and indoor or chained dogs (p < 0.05). Although the prevalence of the E. granulosus infection in owned dogs in Antakya province is low, the incidence was quite high in the unrestrained and undewormed dogs. Therefore a preventive control programme in the dogs should be initiated in this region.

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“…For coproantigen diagnostic, an ELISA was performed in the Parasitic Biology Laboratory of the Hygiene Institute (MontevideoUruguay) as previously described 9 . The cut-off value was determined by calculating the mean optical density (OD) at 492 nm +3 standard deviations.…”

Section: Methodsmentioning

confidence: 99%

Echinococcosis in southern Brazil: efforts toward implementation of a control program in Santana do Livramento, Rio Grande do Sul

Farias

Malgor²,

Cassaravilla³

et al. 2004

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThis investigation aimed to design a strategy for echinococcosis control in Santana do Livramento county, an endemic area in state of Rio Grande do Sul (Brazil). Fecal samples from 65 dogs were obtained from urban, suburban and rural areas. Purging with Arecoline Bromhidrate (AB) was done to visualize Echinococcus granulosus, and Enzyme Linked Immunosorbent Assay (ELISA) was performed to detect parasite coproantigen. Samples were obtained at the beginning and at the end of treatment with Praziquantel. A third fecal sampling was also done in rural areas four months after the end of treatment. Each dog was treated immediately after the first purging and every 30 days for eight months. In urban and suburban areas no infected dogs were found. In rural areas, first evaluation showed 11.36% and 27.69% of infected dogs by AB and ELISA, respectively. No infected dogs were diagnosed in the second evaluation and in the third evaluation 36.84% and 47.37% infected dogs were identified by AB and ELISA, respectively. Medication program to combat dog infection resulted in successful interruption of parasite transmission, but the project failed to create awareness of the need for dog prophylaxis among rural populations as well as to establish a permanent control program in this municipality.

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Coproantigen detection in dogs experimentally and naturally infected with Echinococcus granulosus by a monoclonal antibody-based enzyme-linked immunosorbent assay

Cited by 58 publications

References 5 publications

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

Incidence and the history of Echinococcus granulosus infection in dogs within the past few decades in Libya: A review

Detection of Echinococcus granulosus coproantigens in dogs from Antakya Province, Turkey

Echinococcosis in southern Brazil: efforts toward implementation of a control program in Santana do Livramento, Rio Grande do Sul

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