CoQ10 improves meiotic maturation of pig oocytes through enhancing mitochondrial function and suppressing oxidative stress

Yang, Caixia; Liu, Shuai; Miao, Jia-Kun; Mou, Qiao; Liu, Xiaoman; Wang, Peichao; Huo, Li‐Jun; Du, Zhiqiang

doi:10.1016/j.theriogenology.2020.10.009

Cited by 19 publications

(5 citation statements)

References 38 publications

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“…scRNA-seq helped us to identify the most abundant transcripts in pig oocytes and early IVF/PA embryos, including protein-coding RNAs from both mitochondrial and nuclear genomes. Mitochondria are maternally inherited in mammals 34 , playing an essential role in the regulation of oocyte maturation and early embryo development via oxidative phosphorylation to provide energy 31 , 35 . Mitochondrial mRNAs (except ATP8) gradually increase from MII oocytes to 8-cell embryos in both pig IVF and PA groups, suggesting that active transcription of mitochondrial genome is needed to supply the basic energy requirement for MZT during early embryo development.…”

Section: Discussionmentioning

confidence: 99%

Single cell RNA-seq reveals genes vital to in vitro fertilized embryos and parthenotes in pigs

Liang

Liu

et al. 2021

Sci Rep

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Successful early embryo development requires the correct reprogramming and configuration of gene networks by the timely and faithful execution of zygotic genome activation (ZGA). However, the regulatory principle of molecular elements and circuits fundamental to embryo development remains largely obscure. Here, we profiled the transcriptomes of single zygotes and blastomeres, obtained from in vitro fertilized (IVF) or parthenogenetically activated (PA) porcine early embryos (1- to 8-cell), focusing on the gene expression dynamics and regulatory networks associated with maternal-to-zygote transition (MZT) (mainly maternal RNA clearance and ZGA). We found that minor and major ZGAs occur at 1-cell and 4-cell stages for both IVF and PA embryos, respectively. Maternal RNAs gradually decay from 1- to 8-cell embryos. Top abundantly expressed genes (CDV3, PCNA, CDR1, YWHAE, DNMT1, IGF2BP3, ARMC1, BTG4, UHRF2 and gametocyte-specific factor 1-like) in both IVF and PA early embryos identified are of vital roles for embryo development. Differentially expressed genes within IVF groups are different from that within PA groups, indicating bi-parental and maternal-only embryos have specific sets of mRNAs distinctly decayed and activated. Pathways enriched from DEGs showed that RNA associated pathways (RNA binding, processing, transport and degradation) could be important. Moreover, mitochondrial RNAs are found to be actively transcribed, showing dynamic expression patterns, and for DNA/H3K4 methylation and transcription factors as well. Taken together, our findings provide an important resource to investigate further the epigenetic and genome regulation of MZT events in early embryos of pigs.

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Section: Discussionmentioning

confidence: 99%

Single cell RNA-seq reveals genes vital to in vitro fertilized embryos and parthenotes in pigs

Liang

Liu

et al. 2021

Sci Rep

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“…Similarly, Yang et al. (2021) reported CoQ10 could improve the meiotic maturation of pig oocytes, especially for poor‐quality oocytes. One possible explanation for these facts is that a considerable proportion of IDB supplemented in the IVM medium may not only alleviate the damage of HS but also improve the maturation environment by eliminating the excessive ROS in porcine oocytes, giving rise to a higher blastocyst formation and achieving a better blastocyst quality.…”

Section: Discussionmentioning

confidence: 89%

Idebenone relieves the damage of heat stress on the maturation and developmental competence of porcine oocytes

Shi

Jin

Lin

et al. 2022

Reprod Domestic Animals

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The reproductive function of animals is often affected by climatic conditions. High‐temperature conditions can cause damage to oocyte maturation and embryonic development in a variety of ways. The purpose of this study was to prove that supplementation idebenone (IDB) to the maturation medium can improve the maturation and development of porcine oocytes after heat stress (HS). Porcine cumulus‐oocyte complexes (COCs) were cultured in the maturation medium with different concentrations of IDB (0, 0.1, 1 and 10 μM) for 44 hr at either 38.5°C or under the HS conditions. The cumulus oophorus expansion, nuclear maturation and blastocyst rate after parthenogenetic activation (PA) were measured. We found that HS (in vitro maturation 20–24 hr, 42°C) exposure significantly reduced cumulus expansion index and maturation rate of oocytes and the blastocyst rate of PA embryos, while IDB supplementation significantly improved oocyte maturation and development to the blastocysts stage after PA. Moreover, the addition of IDB decreased the intracellular level of ROS and increased GSH content, hence enhancing the antioxidant capacity of oocytes under HS. Meanwhile, IDB treatment also obviously improved the mitochondrial membrane potential and ATP synthesis of oocytes under HS conditions. Furthermore, IDB treatment increased the expression of GDF9 and BMP15 in IVM oocytes which attribute to improve the quality and outcome of IVM oocytes and the development competence of PA embryos in pigs. In summary, we demonstrated that IDB supplementation into the maturation medium exerted protective effects and improved the ability of maturation and developmental competence of porcine oocytes exposed to HS.

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“…CoQ 10 also improves cytoplasmic maturation by affecting mitochondrial function by improving distribution patterns of mitochondrial, increasing the expression of mitochondrion-associated genes involved in the respiratory chain and metabolism-related genes ( 16 , 18 – 20 , 25 ). Also it improves nuclear maturation via reduction in DNA fragmentation, restoring spindle assembly, chromosome alignment, and actin organization ( 19 , 25 – 27 ). In this regard, it has been investigated that the presence of 30 μM CoQ 10 in the maturation media of sheep oocytes increased the gene expression of Growth Differentiation Factor-9 (GDF-9) as well as follicular development ( 16 , 28 ).…”

Section: Discussionmentioning

confidence: 99%

Expression of Apoptosis-Related Genes in Cultured Ovarian Tissue of Mouse in Presence of Coenzyme Q10

Behnam¹,

Zavareh²,

Salehnia³

2023

JFRH

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Objective: Oxidative stress affects the development of ovarian follicles during in vitro culture thus applying an antioxidant is necessary. The aim of this study was to investigate the effect of coenzyme Q10 (CoQ10) on the expression of apoptosis-related genes of mouse ovaries during in vitro culture. Materials and methods: The immature mouse ovaries were cultured in the presence of 50 μM CoQ10 for 7 days. Histological examinations were performed and the 17 beta-estradiol concentration was measured on the seventh day of culture. The relative expression of Caspase 3, Bax, Bad, and Bcl 2 genes were investigated by real-time RT-PCR. Results: The rates of normal follicles in the presence of CoQ10 was significantly increased compared to the control group. Also, in CoQ10-trated group a significant increase in the level of 17 beta-estradiol was seen compared to the control group. The mRNA expression of anti-apoptotic gene Bcl2 was significantly increased while the expression of pro-apoptotic genes (Caspase3, Bax and Bad) significantly declined in CoQ10 treated group compared to those of control group. Conclusion: The supplementation of the ovarian culture media with CoQ10 improved the follicular development through alteration in expression of apoptosis-related genes and stimulated the production of estradiol.

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CoQ10 improves meiotic maturation of pig oocytes through enhancing mitochondrial function and suppressing oxidative stress

Cited by 19 publications

References 38 publications

Single cell RNA-seq reveals genes vital to in vitro fertilized embryos and parthenotes in pigs

Single cell RNA-seq reveals genes vital to in vitro fertilized embryos and parthenotes in pigs

Idebenone relieves the damage of heat stress on the maturation and developmental competence of porcine oocytes

Expression of Apoptosis-Related Genes in Cultured Ovarian Tissue of Mouse in Presence of Coenzyme Q10

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