Core promoter regulates the expression of cathepsin B gene in the fat body of Bombyx mori

Cai, Xiang-Yun; Yu, Jack; Yu, Haiyan; Liu, Yanwei; Fang, Yin; Ren, Zi-Xu; Jia, Jizeng; Zhang, Guozheng; Guo, Xijie; Jin, Byung-Rae; Gui, Zhongzheng

doi:10.1016/j.gene.2014.03.012

Cited by 11 publications

(16 citation statements)

References 37 publications

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“…Recombinant Autographa californica Multiple Nucleopolyhedrovirus (AcMNPV) Vector-mediated Promoter Activity Analysis in Silkworm Larvae-Recombinant AcMNPVs were generated as described previously (28,29). Briefly, the mutant Phm promoters created using inverse PCR were cloned into the plasmid pFHNLuc-A3RL.…”

Section: Rna Extraction and Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Meng

Cheng

Peng

et al. 2015

Journal of Biological Chemistry

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Background:The transcriptional regulation of steroidogenic enzymes in the silkworm remains poorly understood. Results: Antp and POU-M2 are expressed in the PG and regulate the transcription of Phantom. Conclusion: Antp and POU-M2 coordinate the transcription of Phantom via a protein interaction. Significance: Our study indicates new roles for homeodomain proteins in regulating insect ecdysteroidogenesis.

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Section: Rna Extraction and Quantitative Rt-pcr (Qrt-pcr)mentioning

confidence: 99%

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

Meng

Cheng

Peng

et al. 2015

Journal of Biological Chemistry

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“…It has been confirmed that Bombyx cathepsin B (BmCatB) is involved in the PCD of fat body, as inhibition of BmCatB and Bombyx cathepsin L (BmCatL)‐like (BmCatLL) activities blocks the histolysis of silk gland during Bombyx metamorphosis (Shiba et al ., ; Cai et al ., ). BmCatB and BmCatL are both ecdysone‐responsive, and ecdysone‐response elements (EcREs) exist in their respective promoters (Yu et al ., ; Cai et al ., ). Bombyx fibroinase (BmBcp), another BmCatL‐like protease, extracted from the silk gland, is confirmed to hydrolyse the fibroin molecule and the heavy chain of Bombyx fibroin by acquiring purification protein from silk glands, and its enzymatic properties have been analysed (Sumida et al ., ; Sutthikhum et al ., ; Watanabe et al ., ).…”

Section: Introductionmentioning

confidence: 97%

“…Cysteine-type cathepsins are the main protease family members, including cathepsins B, F, L, L-like, K and O in insects, most of them are genomically identified in Bombyx (Wang et al, 2010;Li et al, 2015). It has been confirmed that Bombyx cathepsin B (BmCatB) is involved in the PCD of fat body, as inhibition of BmCatB and Bombyx cathepsin L (BmCatL)-like (BmCatLL) activities blocks the histolysis of silk gland during Bombyx metamorphosis (Shiba et al, 2001;Cai et al, 2014). BmCatB and BmCatL are both ecdysone-responsive, and ecdysone-response elements (EcREs) exist in their respective promoters (Yu et al, 2012;Cai et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

20‐Hydroxyecdysone‐upregulated proteases involved in Bombyx larval fat body destruction

Guo

et al. 2018

Insect Molecular Biology

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During insect larval-pupal metamorphosis, the obsolete larval organs and tissues undergo histolysis and programmed cell death to recycle cellular materials. It has been demonstrated that some cathepsins are essential for histolysis in larval tissues, but the process of tissue destruction is not well documented. Fat body, the homologous organ to mammalian liver and adipose tissue, goes through a distinct destruction process during larval-pupal transition. Herein, we found that most of the Bombyx proteases - including Bombyx cathepsin B (BmCatB) (BmCatLL-2), Bombyx cathepsin D (BmCatD), Bombyx cathepsin L like-1 (BmCatLL-1) and -2(BmCatLL-2), Bombyx fibroinase (BmBcp), Bombyx matrix metalloprotease (BmMmp), Bombyx A disintegrin and metalloproteinase with thrombospondin motifs 1 (BmAdamTS-1), Bombyx A disintegrin and metalloproteinase with thrombospondin motifs like (BmAdamTS L) and Bombyx cysteine protease inhibitor (Bmbcpi)- were expressed highly in fat body during feeding and metamorphosis, with a peak occurring during the nonfeeding moulting or prepupal stage, as well as being responsive to 20-hydroxyecdysone (20E). The aforementioned protease genes expression was upregulated by injection of 20E into the feeding larvae, while blocking 20E signalling transduction led to downregulation. Western blotting and immunofluorescent staining of BmCatB and BmBcp confirmed the coincident variation of their messenger RNA (mRNA) and protein level during the development and after the treatments. Moreover, BmCatB, BmBcp, BmMmp and BmAdamTS-1 RNA interference all led to blockage of larval fat body destruction. Taken together, we conclude that 20E regulates larval fat body destruction by upregulating related protease gene expression and protein levels during larval-pupal transition.

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“…The domesticated silkworm, Bombyx mori ( B. mori ), which belongs to the Bombycidae family, is a well-studied model species of the Lepidopteran model system with a rich repertoire of genetic information about morphology, development, and behavior [ 9 - 11 ]. Previous studies investigating B. mori have primarily focused on the cloning, expression and characterization of some important genes [ 12 - 14 ], as well as applied research such as bioreactor [ 15 ] or antivirus [ 16 - 18 ]. In addition, there are also some studies on its evolution, which reveal that the domesticated silkworm derives from Chinese stock rather than Japanese or Korean stock [ 19 - 21 ].…”

Section: Introductionmentioning

confidence: 99%

Analysis of codon usage bias of mitochondrial genome in Bombyx moriand its relation to evolution

et al. 2014

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BackgroundSynonymous codon usage bias (SCUB) is an inevitable phenomenon in organismic taxa, generally referring to differences in the occurrence frequency of codons across different species or within the genome of the same species. SCUB happens in various degrees under pressure from nature selection, mutation bias and other factors in different ways. It also attaches great significance to gene expression and species evolution, however, a systematic investigation towards the codon usage in Bombyx mori (B. mori) has not been reported yet. Moreover, it is still indistinct about the reasons contributing to the bias or the relationship between the bias and the evolution of B. mori.ResultsThe comparison of the codon usage pattern between the genomic DNA (gDNA) and the mitochondrial DNA (mtDNA) from B. mori suggests that mtDNA has a higher level of codon bias. Furthermore, the correspondence analysis suggests that natural selection, such as gene length, gene function and translational selection, dominates the codon preference of mtDNA, while the composition constraints for mutation bias only plays a minor role. Additionally, the clustering results of the silkworm superfamily suggest a lack of explicitness in the relationship between the codon usage of mitogenome and species evolution.ConclusionsAmong the complicated influence factors leading to codon bias, natural selection is found to play a major role in shaping the high bias in the mtDNA of B. mori from our current data. Although the cluster analysis reveals that codon bias correlates little with the species evolution, furthermore, a detailed analysis of codon usage of mitogenome provides better insight into the evolutionary relationships in Lepidoptera. However, more new methods and data are needed to investigate the relationship between the mtDNA bias and evolution.

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Core promoter regulates the expression of cathepsin B gene in the fat body of Bombyx mori

Cited by 11 publications

References 37 publications

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

The Homeodomain Transcription Factors Antennapedia and POU-M2 Regulate the Transcription of the Steroidogenic Enzyme Gene Phantom in the Silkworm

20‐Hydroxyecdysone‐upregulated proteases involved in Bombyx larval fat body destruction

Analysis of codon usage bias of mitochondrial genome in Bombyx moriand its relation to evolution

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