Core Regulatory RNA Molecules Identified in Articular Cartilage Stem/Progenitor Cells During Osteoarthritis Progression

Zhang, Shuai; An, Q.; Hu, Peng; Wu, Xiaomin; Pan, Xiaohua; Peng, Wenjin; Wang, Rikang; Gan, Jingyi; Chen, Di; Li, Zhen; Wang, Tianfu; Zhou, Guangqian

doi:10.2217/epi-2018-0212

Cited by 25 publications

(23 citation statements)

References 47 publications

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“…All these features were consistent with previous studies [6,25]. Moreover, our previous studies had confirmed that CPCs had the multilineage differentiation potential [26].…”

Section: Identification Of Cpcs and Cpc-derived Extracellular Vesiclessupporting

confidence: 93%

Intra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model

et al. 2020

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Background: Chondrogenic progenitor cells (CPCs) have high self-renewal capacity and chondrogenic potential. Intra-articular delivery of purified mesenchymal stem cells (MSCs) from MRL/MpJ "superhealer" mice increased bone volume during repair and prevents post-traumatic arthritis. Recently, although extracellular vesicles released from MSCs have been used widely for treating OA, the application of extracellular vesicles secreted by CPCs from MRL/ MpJ mice in OA therapy has never been reported. In this study, we evaluated the effects of extracellular vesicles secreted by CPCs from control CBA (CBA-EVs) and MRL/MpJ mice (MRL-EVs) on proliferation and migration of murine chondrocytes. We also determined here if weekly intra-articular injections of CBA-EVs and MRL-EVs would repair and regenerate surgically induced model in mice. Methods: CPC surface markers were detected by flow cytometry. CBA-EVs and MRL-EVs were isolated using an ultrafiltration method. Nanoparticle tracking analysis, transmission electron microscopy, and western blots were used to identify extracellular vesicles. CBA-EVs and MRL-EVs were injected intra-articularly in a mouse model of surgical destabilization of the medial meniscus (DMM)-induced OA, and histological and immunohistochemistry analyses were used to assess the efficacy of exosome injections. We used miRNA-seq analysis to analyze the expression profiles of exosomal miRNAs derived from CBA-EVs as well as MRL-EVs. Cell-counting and scratch assays were used to evaluate the effects of CBA-EVs and MRL-EVs on proliferation and migration of murine chondrocytes, respectively. Meanwhile, a specific RNA inhibitor assesses the roles of the candidate miRNAs in CPC-EV-induced regulation of function of chondrocytes.

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“…All these features were consistent with previous studies [6,25]. Moreover, our previous studies had confirmed that CPCs had the multilineage differentiation potential [26].…”

Section: Identification Of Cpcs and Cpc-derived Extracellular Vesiclessupporting

confidence: 93%

Intra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model

et al. 2020

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“…CPNE1 is a recently discovered calcium‐dependent membrane‐binding protein with a broad tissue distribution and may regulate signal transduction and membrane trafficking. Dysregulation of CPNE1 is related to various pathologies such as osteoarthritis, neuronal disorders, and tumorigenesis 9‐12,15,20 . However, its role in TNBC tumorigenesis and radioresistance is not understood.…”

Section: Discussionmentioning

confidence: 99%

“…Dysregulation of CPNE1 is related to various pathologies such as osteoarthritis, neuronal disorders, and tumorigenesis. [9][10][11][12]15,20 However, its role in TNBC tumorigenesis and radioresistance is not understood. In this study, CPNE1 expression was increased in BC tissues, especially in TNBC tissues, and correlated with tumor size, distant metastasis, and overall survival of patients with TNBC.…”

Section: Discussionmentioning

confidence: 99%

CPNE1 predicts poor prognosis and promotes tumorigenesis and radioresistance via the AKT singling pathway in triple‐negative breast cancer

Shao

Zhang³

et al. 2020

Molecular Carcinogenesis

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Elevated expression of Copine 1 (CPNE1) has been observed in multiple cancers; however, the underlying mechanisms by which it affects cancer cells are unclear. We aimed to study the effect of CPNE1 on the tumorigenesis and radioresistance of triple‐negative breast cancer (TNBC). Quantitative real‐time polymerase chain reaction was used to detect the expression of CPNE1 in TNBC tissues and cell lines. Western blot, immunohistochemistry, and immunofluorescence were used to investigate the levels of CPNE1, p‐AKT, AKT, cleaved caspase‐3, cleaved PARP1, and γ‐H2AX. Cell viability and apoptosis were measured by CCK‐8 and flow cytometry, respectively. CPNE1 was overexpressed in TNBC tissues and cell lines and was associated with tumor size, distant metastases, and survival rates of patients with TNBC. Moreover, function study shows that CPNE1 promoted cell viability and inhibited cell apoptosis in vitro and inhibited the radiosensitivity of TNBC. Importantly, inactivation of AKT signaling inhibited the tumorigenesis and radioresistance mediated by CPNE1 in TNBC cells. In vivo xenograft study also shows that CPNE1 knockdown inhibited tumor growth and promoted cell apoptosis. Overall, our findings suggest that CPNE1 promotes tumorigenesis and radioresistance in TNBC by regulating AKT activation and targeted CPNE1 expression may be a strategy to sensitize TNBC cells toward radiation therapy.

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“…However, little effort is done aiming to investigate lncRNA expression profile in KOA. With regard to OA, a previous study elucidates that there are 7082 differentially expressed RNAs (including lncRNAs) in mice model of OA, then the OA‐related core RNAs (including lncRNAs) are identified, which are mainly enriched in signaling pathways related to inflammation and bone formation 16 . Another study reveals 2042 differentially expressed lncRNAs in OA cartilage (collected from OA patients) compared with non‐OA cartilage (collected from patients who undergo invasive procedures), including 1137 upregulated lncRNAs and 905 downregulated lncRNAs 17 .…”

Section: Discussionmentioning

confidence: 99%

Retracted: A landscape of circulating long non‐coding RNA (lncRNA) expression profile and the predictive value of candidate lncRNAs for disease risk of knee osteoarthritis

Liu

Zhang

et al. 2020

Clinical Laboratory Analysis

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Background This study aimed to investigate the plasma long non‐coding RNA (lncRNA) expression profile in knee osteoarthritis (KOA) patients and the value of candidate lncRNAs for predicting KOA risk. Methods Plasma was obtained for RNA sequencing (RNA‐seq) in eight KOA patients and eight healthy controls (Ctrls). Ten candidate lncRNAs were then selected from the differentially expressed (DE) lncRNAs according to the rank of absolute value of Log2 (fold change). Afterward, RT‐qPCR was used to examine 10 candidate lncRNAs expressions in plasma of 100 KOA patients and 100 Ctrls. Results In eight KOA patients and eight Ctrls, principal component analysis and heatmap plots disclosed that lncRNA and mRNA expression profile could distinguish KOA patients from Ctrls. Then Volcano plot identified 418 upregulated lncRNAs, 347 downregulated lncRNAs, 521 upregulated mRNAs, and 333 downregulated mRNAs in KOA patients compared to Ctrls. Next, enrichment analyses revealed that DE lncRNAs were mainly enriched in biological processes, molecular functions, and signaling pathways related to inflammation and bone formation. In 100 KOA patients and 100 Ctrls, eight candidate lncRNAs were dysregulated in KOA patients compared to Ctrls, including lncRNA ABCF2P2, lncRNA RP13‐16H11.7, lncRNA CTC‐340A15.2, lncRNA RP4‐735C1.6, lncRNA RP11‐293G6‐B.8, lncRNA RP11‐1246C19.1, lncRNA RP11‐303E16.6, and lncRNA RP5‐882C2.2. Receiver operating characteristic curve analysis revealed that these eight candidate lncRNAs presented with values for predicting KOA risk. Furthermore, multivariate logistic regression elucidated that six candidate lncRNAs could independently predict KOA risk. Conclusion We disclosed a landscape of circulating lncRNA expression profile in KOA patients, and discovered several specific lncRNAs which could assist in KOA management.

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Core Regulatory RNA Molecules Identified in Articular Cartilage Stem/Progenitor Cells During Osteoarthritis Progression

Cited by 25 publications

References 47 publications

Intra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model

Intra-articular delivery of extracellular vesicles secreted by chondrogenic progenitor cells from MRL/MpJ superhealer mice enhances articular cartilage repair in a mouse injury model

CPNE1 predicts poor prognosis and promotes tumorigenesis and radioresistance via the AKT singling pathway in triple‐negative breast cancer

Retracted: A landscape of circulating long non‐coding RNA (lncRNA) expression profile and the predictive value of candidate lncRNAs for disease risk of knee osteoarthritis

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