Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction

Sreejith, Kamalalayam Rajan; Gorgannezhad, Lena; Jin, Jing; Ooi, Chin Hong; Takei, Takayuki; Hayase, Gen; Stratton, Helen; Lamb, Krystina; Shiddiky, Muhammad J. A.; Dao, Dzung Viet; Nguyen, Nam‐Trung

doi:10.3390/mi11030242

Cited by 37 publications

(33 citation statements)

References 43 publications

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“…Our results show that 5 min blue light illumination did not cause severe effect on photobleaching of the PCR products due to the short exposure time and the protective shell. However, our previous studies [28,44] indicated that constant exposure on the sample during thermal cycling over a long period of time can cause photobleaching of amplicons. This issue was solved by non-continuous illumination on the beads during thermal cycling.…”

Section: Fabrication Of the Core-shell Beadmentioning

confidence: 98%

“…The synthesis and characterization of a core-shell bead was reported previously [44]. The synthesis of the bead consists of four main steps.…”

Section: Fabrication Of the Core-shell Beadmentioning

confidence: 99%

“…A custom-built thermal cycler was developed to provide the required conditions for PCR with the core shell beads [44]. A 2 cm × 2 cm × 1.5 cm aluminium block with an embedded cartridge heating element (0.5 cm diameter and 1.5 cm length, core-electronics) served as the heater.…”

Section: Thermal Cyclermentioning

confidence: 99%

“…For programable thermal cycling, a PID (proportional integral derivative) controller was implemented in an Arduino UNO board to provide the closed-loop control of the temperature. This thermal cycler generated the following temperature cycles: initial denaturation at 94 • C for 4 min, 30 cycles of denaturation at 94 • C for 5 sec each and annealing at 59 • C for 20 sec each, 5 min incubation at 72 • C, and infinite hold at 12 • C. More detail on the temperature profile during the PCR process and the control system were reported in our pervious study [44]. .…”

Section: Thermal Cyclermentioning

confidence: 99%

“…The limited number of thermal cycles may negatively impact the amplification efficiency. The team employed a composite liquid marble as a bioreactor to reduce evaporation [44].…”

Section: Introductionmentioning

confidence: 99%

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Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

et al. 2020

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Multiplex polymerase chain reaction (PCR) is an effective tool for simultaneous detection of target genes. Nevertheless, their use has been restricted due to the intrinsic interference between primer pairs. Performing several single PCRs in an array format instead of a multiplex PCR is a simple way to overcome this obstacle. However, there are still major technical challenges in designing a new generation of single PCR microreactors with a small sample volume, rapid thermal cycling, and no evaporation during amplification. We report a simple and robust core-shell bead array for a series of single amplifications. Four core-shell beads with a polymer coating and PCR mixture were synthesized using liquid marble formation and subsequent photo polymerization. Each bead can detect one target gene. We constructed a customised system for thermal cycling of these core-shell beads. Phylogrouping of the E. coli strains was carried out based on the fluorescent signal of the core-shell beads. This platform can be a promising alternative for multiplex nucleic acid analyses due to its simplicity and high throughput. The platform reported here also reduces the cycling time and avoids evaporation as well as contamination of the sample during the amplification process.

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Section: Fabrication Of the Core-shell Beadmentioning

confidence: 98%

“…The synthesis and characterization of a core-shell bead was reported previously [44]. The synthesis of the bead consists of four main steps.…”

Section: Fabrication Of the Core-shell Beadmentioning

confidence: 99%

Section: Thermal Cyclermentioning

confidence: 99%

Section: Thermal Cyclermentioning

confidence: 99%

“…The limited number of thermal cycles may negatively impact the amplification efficiency. The team employed a composite liquid marble as a bioreactor to reduce evaporation [44].…”

Section: Introductionmentioning

confidence: 99%

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Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

et al. 2020

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Long‐Lived Liquid Marbles for Green Applications

Saczek

Yao

Živković

et al. 2021

Adv Funct Materials

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Liquid marbles allow for quantities of various liquids to be encapsulated by hydrophobic particles, thus ensuring isolation from the external environment. The unique properties provided by this soft solid has allowed for use in a wide array of different applications. Liquid marbles do however have certain drawbacks, with lifetime and robustness often being limited. Within this review, particle characteristics that impact liquid marble stability are critically discussed, in addition to other factors, such as internal and external environments, that can be engineered to achieve a robust long-lived liquid marble. New emerging applications, which will benefit from this improvement, are explored such as unconventional computing, cell mimicry, and soft lithography. Incorporation of liquid marbles and liquid crystal technologies shows promise in utilizing structural color for optical display applications, and within green and environmental applications, liquid marble technology is increasingly adapted for use in energy conversion, heavy metal recovery, CO 2 capture, and oil removal.

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Formation of core–shell droplets for the encapsulation of liquid contents

Galogahi

Zhu

et al. 2021

Microfluid Nanofluid

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Accurate control of monodisperse core-shell droplets generated in a microfluidic device has a broad range of applications in research and industry. This paper reports the experimental investigation of flow-focusing microfluidic devices capable of producing size-tuneable and monodisperse core-shell droplets. The dimension of the core-shell droplets was controlled passively by the channel geometry and the flow rate of the liquid phases. The results indicate that microchannel geometry is more significant than flow rates. The highly controllable core-shell droplets could be subsequently employed as a template for generating core-shell micropaticles with liquid core.Optical, electron microscopy and X-ray computed microtomography showed that the geometry of the core-shell droplets remains unchanged after solidification, drying and collection. The present study also looks at the thermal stability of core-shell particles depending on the particle size. The larger core-shell partcles with a thicker shell provide a higher resistance to heating at elevated temperature. The high degree of control with a flow-focusing microfluidic device makes this a promising approach for the encapsulation, storage, and delivery of lipophilic contents.

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Core-Shell Beads Made by Composite Liquid Marble Technology as A Versatile Microreactor for Polymerase Chain Reaction

Cited by 37 publications

References 43 publications

Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

Core-Shell Beads as Microreactors for Phylogrouping of E. coli Strains

Long‐Lived Liquid Marbles for Green Applications

Formation of core–shell droplets for the encapsulation of liquid contents

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