Core-shell PLA/Kef hybrid scaffolds for skin tissue engineering applications prepared by direct kefiran coating on PLA electrospun fibers optimized via air-plasma treatment

Lopresti, Francesco; Campora, Simona; Tirri, Giorgia; Capuana, Elisa; Pavia, Francesco Carfì; Brucato, V.; Ghersi, Giulio; Carrubba, Vincenzo La

doi:10.1016/j.msec.2021.112248

Cited by 36 publications

(37 citation statements)

References 57 publications

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“…Nitrogen-fractured scaffolds were attached by using adhesive carbon tape on an aluminum stub. Before the analysis, the samples were sputter-coated with gold for 60 s under an argon atmosphere by using a Sputtering Scancoat Six (Edwards Laboratories, Milpitas, CA, USA) in order to avoid electrostatic charge during the test [11].…”

Section: Morphological Analysismentioning

confidence: 99%

“…where ∆H cc and ∆H m are the cold crystallization and melting enthalpy of the samples, respectively. X i is the weight fraction of PLA or PHA, and ∆H 0 i is the melting enthalpy of 100% crystalline PLLA or PHA equal to 93.7 J/g [11] and 145 J/g [50], respectively.…”

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

“…Over the recent years, large technological and scientific interest have dealt with the possibility of controlling polymer foams products to be employed as scaffolds for tissue engineering applications [1][2][3]. Many techniques have been developed to produce porous tissue engineering scaffolds, such as porogen leaching [4,5], freeze drying [6,7], 3D printing [8][9][10], electrospinning [11][12][13], thermally induced phase separation (TIPS) [14][15][16] and any possible combinations of these [17]. Among the listed techniques, TIPS is one of the most efficient due to its ease of implementation and potential capability to produce highly porous scaffolds with tunable properties [15,17].…”

Section: Introductionmentioning

confidence: 99%

“…Generally, natural biopolymers show enzyme-controlled degradability, good biocompatibility, low inflammatory potential, high chemical versatility and similarities with the extracellular matrix [34,35]. For this reason, a variety of natural biopolymer-based scaffolds composed of alginate, dextran, hyaluronic acid, kefiran and chitosan, among others, have been recently used in tissue engineering and regenerative medicine strategies due to their excellent biocompatibility combined with their potential biodegradability [11,34,[36][37][38][39]. Polyhydroxyalkanoates (PHAs) are a family of biodegradable polyesters intracellularly produced by many microorganisms as carbon and energy storage compounds under unbalanced growth conditions [40,41].…”

Section: Introductionmentioning

confidence: 99%

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Effect of Polyhydroxyalkanoate (PHA) Concentration on Polymeric Scaffolds Based on Blends of Poly-L-Lactic Acid (PLLA) and PHA Prepared via Thermally Induced Phase Separation (TIPS)

et al. 2022

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Hybrid porous scaffolds composed of both natural and synthetic biopolymers have demonstrated significant improvements in the tissue engineering field. This study investigates for the first time the fabrication route and characterization of poly-L-lactic acid scaffolds blended with polyhydroxyalkanoate up to 30 wt%. The hybrid scaffolds were prepared by a thermally induced phase separation method starting from ternary solutions. The microstructure of the hybrid porous structures was analyzed by scanning electron microscopy and related to the blend composition. The porosity and the wettability of the scaffolds were evaluated through gravimetric and water contact angle measurements, respectively. The scaffolds were also characterized in terms of the surface chemical properties via Fourier transform infrared spectroscopy in attenuated total reflectance. The mechanical properties were analyzed through tensile tests, while the crystallinity of the PLLA/PHA scaffolds was investigated by differential scanning calorimetry and X-ray diffraction.

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Section: Morphological Analysismentioning

confidence: 99%

Section: Differential Scanning Calorimetrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of Polyhydroxyalkanoate (PHA) Concentration on Polymeric Scaffolds Based on Blends of Poly-L-Lactic Acid (PLLA) and PHA Prepared via Thermally Induced Phase Separation (TIPS)

et al. 2022

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“…Another notable application of scaffolds is their use as supporting materials for different drug loadings, allowing for the sustained and controlled release of drugs over the desired time period [ 26 , 27 ]. Several engineered systems have been investigated for this purpose, including natural materials such as gelatin [ 28 ], zein [ 29 ], bovine serum albumin (BSA) [ 30 ], kefiran [ 31 , 32 ] and chitosan [ 33 ] or synthetic polymers such as polycaprolactone (PCL) [ 34 ], poly (lactic-co-glycolic acid) (PLGA) [ 35 ], and PLA [ 36 ]. For these therapeutic applications, a low rate of biodegradation is required [ 30 , 37 ], as observed for PLLA.…”

Section: Introductionmentioning

confidence: 99%

Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications

et al. 2022

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Synthetic biopolymers are effective cues to replace damaged tissue in the tissue engineering (TE) field, both for in vitro and in vivo application. Among them, poly-l-lactic acid (PLLA) has been highlighted as a biomaterial with tunable mechanical properties and biodegradability that allows for the fabrication of porous scaffolds with different micro/nanostructures via various approaches. In this review, we discuss the structure of PLLA, its main properties, and the most recent advances in overcoming its hydrophobic, synthetic nature, which limits biological signaling and protein absorption. With this aim, PLLA-based scaffolds can be exposed to surface modification or combined with other biomaterials, such as natural or synthetic polymers and bioceramics. Further, various fabrication technologies, such as phase separation, electrospinning, and 3D printing, of PLLA-based scaffolds are scrutinized along with the in vitro and in vivo applications employed in various tissue repair strategies. Overall, this review focuses on the properties and applications of PLLA in the TE field, finally affording an insight into future directions and challenges to address an effective improvement of scaffold properties.

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Effect of core–sheath bi‐polymeric scaffolds fabricated from acid‐soluble collagen and poly(lactic acid) derivatives on wound healing

Mukta,

Ahmed,

Sarwaruddin Chowdhury

et al. 2024

J of Applied Polymer Sci

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The core–sheath bi‐polymeric scaffold has been proven as an encouraging material based on the requirement of scaffolds. This study aims to prepare electrospun core–sheath scaffolds by using acid‐soluble collagen (ASC) as core material and poly(lactic acid) (PLA) or PLA‐g‐VAc as sheath material to get the most in combination from a hydrophilic and a hydrophobic polymer. ASC is extracted from waste Tilapia fish skin conserving the triple helix structure of the α1 (130 kDa) chain, and a α2 (120 kDa) chain cross‐linked with the β (280 kDa) chain confirmed by amino acid profile, sodium dodecyl sulphate‐polyacrylamide gel electrophoresis. PLA‐g‐VAc is prepared by grafting vinyl acetate (VAc) onto the PLA chain using benzoyl peroxide as the initiator. FT‐IR, 1H NMR, and 13C NMR of PLA‐g‐VAc reveal that grafting occurs between the double bond of VAc and the methine group of PLA. The morphology of the scaffolds is determined by the field emission scanning electron microscope. FT‐IR, thermogravimetric analysis, differential scanning calorimetry, XRD, and water contact angle measurements are used for further characterization of scaffolds. In vivo, cytotoxicity analysis on the Vero cell line exposes that scaffolds are biocompatible. Application of scaffolds to the surgically produced wounding of skin in a rat model followed by histological assay indicates the enhanced properties of core–sheath scaffolds rather than the single polymeric scaffolds.

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Core-shell PLA/Kef hybrid scaffolds for skin tissue engineering applications prepared by direct kefiran coating on PLA electrospun fibers optimized via air-plasma treatment

Cited by 36 publications

References 57 publications

Effect of Polyhydroxyalkanoate (PHA) Concentration on Polymeric Scaffolds Based on Blends of Poly-L-Lactic Acid (PLLA) and PHA Prepared via Thermally Induced Phase Separation (TIPS)

Effect of Polyhydroxyalkanoate (PHA) Concentration on Polymeric Scaffolds Based on Blends of Poly-L-Lactic Acid (PLLA) and PHA Prepared via Thermally Induced Phase Separation (TIPS)

Poly-l-Lactic Acid (PLLA)-Based Biomaterials for Regenerative Medicine: A Review on Processing and Applications

Effect of core–sheath bi‐polymeric scaffolds fabricated from acid‐soluble collagen and poly(lactic acid) derivatives on wound healing

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