Corn stover lignin is modified differently by acetic acid compared to sulfuric acid

Mouthier, Thibaut M.B.; Appeldoorn, Maaike M.; Pel, Herman J.; Schols, Henk A.; Gruppen, Harry; Kabel, Mirjam A.

doi:10.1016/j.indcrop.2018.05.008

Cited by 17 publications

(7 citation statements)

References 36 publications

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“…In this work, such a low glucose yield (35.2%) was noticed in the hydrolysis of deacylated AC-pretreated poplar, which indicated that the acetylation of poplar might be not responsible for the main reason of the low hydrolysis yield of AC-pretreated poplar. Thus, the high residual lignin contents (25.1–36.1%) in the AC-pretreated poplar might be responsible for the relatively low hydrolysis yield [36, 37].…”

Section: Resultsmentioning

confidence: 99%

Production of xylooligosaccharides and monosaccharides from poplar by a two-step acetic acid and peroxide/acetic acid pretreatment

Wen

Tian

Wang

et al. 2019

Biotechnol Biofuels

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Background Populus (poplar) tree species including hybrid varieties are considered as promising biomass feedstock for biofuels and biochemicals production due to their fast growing, short vegetative cycle, and widely distribution. In this work, poplar was pretreated with acetic acid (AC) to produce xylooligosaccharides (XOS), and hydrogen peroxide–acetic acid (HPAC) was used to remove residual lignin in AC-pretreated poplar for enzymatic hydrolysis. The aim of this work is to produce XOS and monosaccharides from poplar by a two-step pretreatment method. Results The optimal conditions for the AC pretreatment were 170 °C, 5% AC, and 30 min, giving a XOS yield of 55.8%. The optimal HPAC pretreatment conditions were 60 °C, 2 h, and 80% HPAC, resulting in 92.7% delignification and 87.8% cellulose retention in the AC-pretreated poplar. The two step-treated poplar presented 86.6% glucose yield and 89.0% xylose yield by enzymatic hydrolysis with a cellulases loading of 7.2 m/g dry mass. Very high glucose (93.8%) and xylose (94.6%) yields were obtained with 14.3 mg cellulases/g dry mass. Both Tween 80 and β-glucosidase enhanced glucose yield of HPAC-pretreated poplar by alleviating the accumulation of cellobiose. Under the optimal conditions, 6.9 g XOS, 40.3 g glucose, and 8.9 g xylose were produced from 100 g poplar. Conclusions The AC and HPAC pretreatment of poplar represented an efficient strategy to produce XOS and fermentable sugars with high yields. This two-step pretreatment was a recyclable benign and advantageous scheme for biorefinery of the poplar into XOS and monosaccharides. Electronic supplementary material The online version of this article (10.1186/s13068-019-1423-x) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Production of xylooligosaccharides and monosaccharides from poplar by a two-step acetic acid and peroxide/acetic acid pretreatment

Wen

Tian

Wang

et al. 2019

Biotechnol Biofuels

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“…Cellulolytic enzymes produced by T. reesei are composed of two cellobiohydrolases (CBHs: Cel7A and Cel6A), six endoglucanases (EGs), and small amounts of other enzymes such as β-glucosidase, six xylanase types (XYLI~VI), β-xylosidase, xyloglucanase, and mannanase [ 34 , 35 , 36 , 37 ]. The CBH f (full length of cellobiohydrolases) consists of a catalytic domain (CD) and a carbohydrate binding motif (CBM) and cleaves off cellobiose units from the reducing and non-reducing ends.…”

Section: Resultsmentioning

confidence: 99%

Comparative Evaluation of Adsorption of Major Enzymes in a Cellulase Cocktail Obtained from Trichoderma reesei onto Different Types of Lignin

et al. 2022

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Cellulase adsorption onto lignin decreases the productivity of enzymatic hydrolysis of lignocellulosic biomass. Here, adsorption of enzymes onto different types of lignin was investigated, and the five major enzymes—cellobiohydrolases (CBHs), endoglucanase (Cel7B), β-glucosidase (Cel3A), xylanase (XYNIV), and mannanase (Man5A)—in a cellulase cocktail obtained from Trichoderma reesei were individually analyzed through SDS-PAGE and zymogram assay. Lignin was isolated from woody (oak and pine lignin) and herbaceous (rice straw and kenaf lignin) plants. The relative adsorption of CBHs compared to the control was in the range of 14.15–18.61%. The carbohydrate binding motif (CBM) of the CBHs contributed to higher adsorption levels in oak and kenaf lignin, compared to those in pine and rice lignin. The adsorption of endoglucanase (Cel7B) by herbaceous plant lignin was two times higher than that of woody lignin, whereas XYNIV showed the opposite pattern. β-glucosidase (Cel3A) displayed the highest and lowest adsorption ratios on rice straw and kenaf lignin, respectively. Mannanase (Man5A) was found to have the lowest adsorption ratio on pine lignin. Our results showed that the hydrophobic properties of CBM and the enzyme structures are key factors in adsorption onto lignin, whereas the properties of specific lignin types indirectly affect adsorption.

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“…Generally it has been applied as an acetic acid-water system catalyzed by HCl around 100 • C and 2 h of time [27]. However, under such conditions it has been previously found that the lignin, dissolved during such processes, can condense back during the cooling process [28].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Lignin Extraction Variables by Response Surface Methodology from Pine Saw Dust, and Quantification of Major Structural Units in Isolated Lignin Fraction

2021

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A synergistic combination of dioxane, acetic acid, and HCl was investigated for lignin extraction from pine wood biomass. After initial screening of reagent combination, response surface methodology (RSM) was used to optimize the lignin yield with respect to the variables of time 24–72 h, solids loading 5–15%, and catalyst dose 5–15 mL. A quadratic model predicted 8.33% of the lignin yield, and it was further confirmed experimentally and through the analysis of variance (ANOVA). Lignin at optimum combination exhibited features in terms of derivatization followed by reductive cleavage (DFRC) with a value of (305 µmol/gm), average molecular weights of 4358 and polydispersity of 1.65, and 2D heteronuclear single quantum coherence nuclear magnetic resonance spectrum (2D-HSQC NMR) analysis showing relative β-O-4 linkages (37.80%). From here it can be suggested that this fractionation can be one option for high quality lignin extraction from lignocellulosic biomass.

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Corn stover lignin is modified differently by acetic acid compared to sulfuric acid

Cited by 17 publications

References 36 publications

Production of xylooligosaccharides and monosaccharides from poplar by a two-step acetic acid and peroxide/acetic acid pretreatment

Production of xylooligosaccharides and monosaccharides from poplar by a two-step acetic acid and peroxide/acetic acid pretreatment

Comparative Evaluation of Adsorption of Major Enzymes in a Cellulase Cocktail Obtained from Trichoderma reesei onto Different Types of Lignin

Optimization of Lignin Extraction Variables by Response Surface Methodology from Pine Saw Dust, and Quantification of Major Structural Units in Isolated Lignin Fraction

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