Corneal Endothelial Transplantation

McCulley, James P.; Maurice, David M.; Schwartz, Barry

doi:10.1016/s0161-6420(80)35259-7

Cited by 74 publications

(37 citation statements)

References 4 publications

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“…At an experimental approach, CEC transplantation is investigated at an in vitro level [Alvarado et al, 1981;Bednarz et al, 1996;Aboalchamat et al, 1999;Bohnke et al, 1999;Engelmann et al, 1999;Chen et al, 2001] and in animal studies [Jumblatt et al, 1978;Gospodarowicz et al, 1979a, b;McCulley et al, 1980;Joo et al, 2000;Ishino et al, 2004;Mimura et al, 2004b;Hsiue et al, 2006] using cell suspensions or engineered cell sheets of cultured CEC. Several groups could demonstrate that cultured HCEC can be transplanted onto denuded donor corneas, where they establish a new functional monolayer [Aboalchamat et al, 1999;Bohnke et al, 1999;Engelmann et al, 1999;Chen et al, 2001;Amano, 2003;Mimura et al, 2004a].…”

Section: Clonal Cell Lines Of Human Corneal Endothelium Cells Tissuesmentioning

confidence: 99%

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics

Valtink

Gruschwitz

Funk

et al. 2008

Cells Tissues Organs

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Access to primary human corneal endothelial cells (HCEC) is limited and donor-derived differences between cultures exacerbate the issue of data reproducibility, whereas cell lines can provide sufficient numbers of homogenous cells for multiple experiments. An immortalized HCEC population was adapted to serum-free culture medium and repeated cloning was performed. Clonally grown cells were propagated under serum-free conditions and growth curves were recorded. Cells were characterized immunocytochemically for junctional proteins, collagens, Na,K-ATPase and HCEC-specific 9.3.E-antigen. Ultrastructure was monitored by scanning and transmission electron microscopy. Two clonal cell lines, HCEC-B4G12 and HCEC-H9C1, could be isolated and expanded, which differed morphologically: B4G12 cells were polygonal, strongly adherent and formed a strict monolayer, H9C1 cells were less adherent and formed floating spheres. The generation time of B4G12 cells was 62.26 ± 14.5 h and that of H9C1 cells 44.05 ± 5.05 h. Scanning electron microscopy revealed that B4G12 cells had a smooth cell surface, while H9C1 cells had numerous thin filopodia. Both cell lines expressed ZO-1 and occludin adequately, and little but well detectable amounts of connexin-43. Expression of HCEC-specific 9.3.E-antigen was found commensurately in both cell lines, while expression of Na,K-ATPase α1 was higher in H9C1 cells than in B4G12 cells. B4G12 cells expressed collagen IV abundantly and almost no collagen III, while H9C1 cells expressed both collagens at reasonable amounts. It is concluded that the clonal cell line B4G12 represents an ideal model of differentiated HCEC, while H9C1 may reflect features of developing or transitional HCEC.

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Section: Clonal Cell Lines Of Human Corneal Endothelium Cells Tissuesmentioning

confidence: 99%

Two Clonal Cell Lines of Immortalized Human Corneal Endothelial Cells Show either Differentiated or Precursor Cell Characteristics

Valtink

Gruschwitz

Funk

et al. 2008

Cells Tissues Organs

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“…[1][2][3][4] Several groups have developed techniques for primary culture and serial passaging of HCEC from embryos 5 or donor corneas of all ages. [6][7][8][9] We have also succeeded in primary culture and serial passaging of HCEC lines from a wide range of donor tissues.…”

Section: Introductionmentioning

confidence: 99%

“…[6][7][8][9] We have also succeeded in primary culture and serial passaging of HCEC lines from a wide range of donor tissues. 10 Moreover, several groups have demonstrated the capacity of endothelial cells to survive and function properly when transplanted onto a nonnative cornea in species possessing 1,2,4,11 or lacking 3 regenerative capacity of their native corneal endothelium. Furthermore, recent attempts to transplant HCEC to monkey 12,13 and human recipients 14,15 have been successful.…”

Section: Introductionmentioning

confidence: 99%

Properties of Corneas Reconstructed with Cultured Human Corneal Endothelial Cells and Human Corneal Stroma

et al. 2005

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“…Tradicionalmente, o tratamento desta disfunção requer a troca de toda espessura corneana (transplante penetrante), cujas complicações já são bem conhecidas e são relacionadas principalmente às suturas (3)(4) . Outras alternativas de tratamento têm sido desenvolvidas nos últimos 25 anos, como implante de células endoteliais (5)(6) , transplante endotelial via lamela anterior (7) ou via incisão límbica (8)(9)(10) . Na ceratoplastia endotelial lamelar posterior (CELP), na qual um disco lamelar de estroma fino, membrana de Descemet e endotélio são transplantados sem sutura corneana, espera-se a manutenção da ceratometria do próprio paciente (11) e a ocorrência de menos complicações relacionadas ao astigmatismo, às suturas, às infecções e à cicatrização (12)(13) .…”

Section: Introductionunclassified