Corneal regeneration by conditioned medium of human uterine cervical stem cells is mediated by TIMP-1 and TIMP-2

Sendón-Lago, Juan; Seoane, Samuel; Martínez-Ordóñez, Anxo; Eiró, Noemí; Saá, Jorge; Vizoso, F; González, Francisco; Perez-Fernandez, Román; Bermúdez, María A.

doi:10.1016/j.exer.2018.12.004

Cited by 28 publications

(32 citation statements)

References 40 publications

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“…In Albino Rabbit, 0.1% BAC eye drop is applied to induce dry eye ( 51 – 53 ). Another study used 1% atropine sulfate to instill into the eyes three times a day for 3 days ( 54 ). For Wister rats, Joossen et al, and Park et al, induced dry eye by removing the lacrimal gland ( 55 , 56 ).…”

Section: Methodsmentioning

confidence: 99%

Cluster Analysis of Dry Eye Disease Models Based on Immune Cell Parameters – New Insight Into Therapeutic Perspective

2020

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Dry eye disease (DED) can be represented as a display of disease in the mucosal part of the eye. It is quite distinct from the retinal side of the eye which connects with the neurons and thus represents the neuroimmunological disease. DED can occur either by the internal damage of the T cells inside the body or by microbial infections. Here we summarize the most common animal model systems used for DED relating to immune factors. We aimed to identify the most important immune cell/cytokine among the animal models of the disease. We also show the essential immune factors which are being tested for DED treatment. In our results, both the mechanism and the treatment of its animal models indicate the involvement of Th1 cells and the pro-inflammatory cytokine (IL-1β and TNF-α) related to the Th1-cells. The study is intended to increase the knowledge of the animal models in the field of the ocular surface along with the opening of a dimension of thoughts while designing a new animal model or treatment paradigm for ocular surface inflammatory disorders.

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Section: Methodsmentioning

confidence: 99%

Cluster Analysis of Dry Eye Disease Models Based on Immune Cell Parameters – New Insight Into Therapeutic Perspective

2020

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“…Thus, for example, it was reported that conditioned medium of hUCESC reduce leukocyte infiltration in ocular tissues after endotoxin-induced uveitis, similar to that achieved with dexamethasone treatment ( Bermudez et al, 2016 ). It also inhibits and reverse monocyte differentiation to macrophages, greater than inhibition and reversion achieved with AD-MSC ( Eiró et al, 2014 ), and also reduce tissue levels of IL-6, IL-8, IFN-γ, TNF-α, MCP-1, or MIP-1α mRNA, as well as increase of the anti-inflammatory interleukin IL-10 (at levels comparable to those achieved with dexamethasone treatment) ( Bermudez et al, 2015 , 2016 ; Sendon-Lago et al, 2019 ).…”

Section: Importance Of the Msc Heterogeneity For Future Therapiesmentioning

confidence: 98%

“…Human uterine cervical stem cells (hUCESC) have a high proliferative rate ( Eiró et al, 2014 ) and its secretome has potent regenerative ( Bermudez et al, 2015 ; Sendon-Lago et al, 2019 ), anti-inflammatory ( Eiró et al, 2014 ; Bermudez et al, 2016 ), anti-tumor ( Eiró et al, 2014 ) and anti-microbial capacities ( Bermudez et al, 2015 ; Schneider et al, 2018 ). The comparison of cytokines profile from secretome of AD-MSC (one of the most MSC type used in clinical trials) and of hUCESC shows that the latter has higher levels of cytokines with recognized effect in regenerative (TIMP-1, TIMP-2, FGF-6, FGF-7, uPAR, and HGF) ( Bermudez et al, 2015 ; Sendon-Lago et al, 2019 ), anti-tumoral (FLT-3 ligand, LAP, LIGHT, and IP-10) ( Eiró et al, 2014 ), and anti-inflammatory (IL-13 and NT-3) ( Eiró et al, 2014 ; Bermudez et al, 2016 ) processes.…”

Section: Importance Of the Msc Heterogeneity For Future Therapiesmentioning

confidence: 99%

The Coronavirus Pandemic (SARS-CoV-2): New Problems Demand New Solutions, the Alternative of Mesenchymal (Stem) Stromal Cells

Eiró

Cabrera

Fraile

et al. 2020

Front. Cell Dev. Biol.

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Mesenchymal (stem) stromal cells (MSC) can be a therapeutic alternative for COVID-19 considering their anti-inflammatory, regenerative, angiogenic, and even antimicrobial capacity. Preliminary data point to therapeutic interest of MSC for patients with COVID-19, and their effect seems based on the MSC's ability to curb the cytokine storm caused by COVID-19. In fact, promising clinical studies using MSC to treat COVID-19, are currently underway. For this reason, now is the time to firmly consider new approaches to MSC research that addresses key issues, like selecting the most optimal type of MSC for each indication, assuming the heterogeneity of the donor-dependent MSC and the biological niche where MSC are located.

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“…Scaffold-free cell delivery attracts scientists because it eliminates the need for any additional biomaterial for tissue regeneration [41,42]. Since autologous blood derivatives such as autologous serum (AS) and plasma rich in growth factors (PRGFs) have noticeable advantages such as availability, no risk of rejection and biocompatibility, they Preclinical [54,55] Conditioned medium from induced pluripotent stem cells…”

Section: Corneal Regeneration By Scaffold-free Cell or Growth Factor mentioning

confidence: 99%

“…Furthermore, topical use of conditioned medium from human uterine cervical stem cells (CM-hUCES) was shown to have effective impacts on treating dry eye and corneal wound in rabbit models. It was observed that tissue inhibitor of metalloproteinase-1 and -2 (TIMP) which were abundant in CM-hUCESC enhanced regeneration by increasing corneal epithelial cell proliferation and avoiding apoptosis [54]. Besides wound healing, CM-hUCESs were observed to have anti-inflammatory and antibacterial effects which made it a good candidate for the contact lens solution to avoid any infection [55].…”

Section: Growth Factor Deliverymentioning

confidence: 99%

Bioengineering Approaches for Corneal Regenerative Medicine

Mahdavi

Abdekhodaie

Mashayekhan

et al. 2020

Tissue Eng Regen Med

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BACKGROUND: Since the cornea is responsible for transmitting and focusing light into the eye, injury or pathology affecting any layer of the cornea can cause a detrimental effect on visual acuity. Aging is also a reason for corneal degeneration. Depending on the level of the injury, conservative therapies and donor tissue transplantation are the most common treatments for corneal diseases. Not only is there a lack of donor tissue and risk of infection/rejection, but the inherent ability of corneal cells and layers to regenerate has led to research in regenerative approaches and treatments. METHODS: In this review, we first discussed the anatomy of the cornea and the required properties for reconstructing layers of the cornea. Regenerative approaches are divided into two main categories; using direct cell/growth factor delivery or using scaffold-based cell delivery. It is expected delivered cells migrate and integrate into the host tissue and restore its structure and function to restore vision. Growth factor delivery also has shown promising results for corneal surface regeneration. Scaffold-based approaches are categorized based on the type of scaffold, since it has a significant impact on the efficiency of regeneration, into the hydrogel and non-hydrogel based scaffolds. Various types of cells, biomaterials, and techniques are well covered. RESULTS: The most important characteristics to be considered for biomaterials in corneal regeneration are suitable mechanical properties, biocompatibility, biodegradability, and transparency. Moreover, a curved shape structure and spatial arrangement of the fibrils have been shown to mimic the corneal extracellular matrix for cells and enhance cell differentiation. CONCLUSION: Tissue engineering and regenerative medicine approaches showed to have promising outcomes for corneal regeneration. However, besides proper mechanical and optical properties, other factors such as appropriate sterilization method, storage, shelf life and etc. should be taken into account in order to develop an engineered cornea for clinical trials.

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Corneal regeneration by conditioned medium of human uterine cervical stem cells is mediated by TIMP-1 and TIMP-2

Cited by 28 publications

References 40 publications

Cluster Analysis of Dry Eye Disease Models Based on Immune Cell Parameters – New Insight Into Therapeutic Perspective

Cluster Analysis of Dry Eye Disease Models Based on Immune Cell Parameters – New Insight Into Therapeutic Perspective

The Coronavirus Pandemic (SARS-CoV-2): New Problems Demand New Solutions, the Alternative of Mesenchymal (Stem) Stromal Cells

Bioengineering Approaches for Corneal Regenerative Medicine

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