Cornstarch-based wound dressing incorporated with hyaluronic acid and propolis: In vitro and in vivo studies

Eskandarinia, Asghar; Kefayat, Amirhosein; Rafienia, Mohammad; Agheb, Maria; Navid, Sepehr; Ebrahimpour, Karim

doi:10.1016/j.carbpol.2019.03.091

Cited by 87 publications

(58 citation statements)

References 63 publications

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“…Shahr-e Kord suburbs' beehives were selected for collection of propolis. The ethanolic extract of propolis (EEP) was prepared according to previous our study 28 . The harvested propolis was frozen at −20 for 24 h and then crushed in a blender.…”

Section: Preparation Of Ethanolic Extract Of Propolis (Eep)mentioning

confidence: 99%

“…Hydrolytic and enzymatic degradation. Stability of the PU/EEP and PCL/Gel wound dressings against hydrolytic and enzymatic degradation were investigated according to our previous study 30 . Briefly, the PU/EEP (weight: 0.25284 ± 0.02658 g, diameters: 50 mm × 15 mm, n = 9) and PCL/Gel (weight: 0.03618 ± 0.00334 g, diameters: 50 mm × 15 mm, n = 9) samples were immersed in 10 mL of PBS solution (pH 7.4, 37 °C) for 28 days, to evaluate its stability in an aqueous medium (hydrolytic degradation).…”

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

“…propolis release. The propolis release from the PU/EEP membrane was carried according to our previous study 30 . Briefly, the membrane was immersed in PBS under oscillation and the propolis concentration was measured using high-performance liquid chromatography at different time points.…”

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

“…Then, incubation with 2 vol% glutaraldehyde aqueous solution for 1 h was used for fixing the samples. After multiple times PBS washing, the fixed samples were dehydrated in a graded concentration of ethanol (30,50,70,80,90, 95, 100%) and dried. The dried samples were immediately sputter-coated with gold and the cells morphology was examined under scanning electron microscope (SEM).…”

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

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A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Eskandarinia

Kefayat

Agheb

et al. 2020

Sci Rep

Self Cite

155

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one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are composed of two layers with different properties have gained lots of attention. in the present study, polycaprolactone/gelatin (pcL/Gel) scaffold was electrospun on a dense membrane composed of polyurethane and ethanolic extract of propolis (pU/eep). the pU/eep membrane was used as the top layer to protect the wound area from external contamination and dehydration, while the PCL/Gel scaffold was used as the sublayer to facilitate cells' adhesion and proliferation. the bilayer wound dressing was investigated regarding its microstructure, mechanical properties, surface wettability, anti-bacterial activity, biodegradability, biocompatibility, and its efficacy in the animal wound model and histopathological analyzes. Scanning electron micrographs exhibited uniform morphology and bead-free structure of the PCL/Gel scaffold with average fibers' diameter of 237.3 ± 65.1 nm. Significant anti-bacterial activity was observed against Staphylococcal aureus (5.4 ± 0.3 mm), Escherichia coli (1.9 ± 0.4 mm) and Staphylococcus epidermidis (1.0 ± 0.2 mm) according to inhibition zone test. The bilayer wound dressing exhibited high hydrophilicity (51.1 ± 4.9°), biodegradability, and biocompatibility. The bilayer wound dressing could significantly accelerate the wound closure and collagen deposition in the Wistar rats' skin wound model. taking together, the pU/eep-pcL/Gel bilayer wound dressing can be a potential candidate for biomedical applications due to remarkable mechanical properties, biocompatibility, antibacterial features, and wound healing activities. Skin is always at the exposer of different types of damages 1. Severe skin damages can be life-threatening due to loss of human body fluids, electrolytes, and nutritional components from the wound area. Therefore, wound dressings have gained lots of attention 2. An ideal wound dressing should protect the wound from external contaminants and facilitate the healing process. However, one-layer wound dressings cannot meet all the clinical needs due to their individual characteristics and shortcomings. Therefore, bilayer wound dressings which are

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Section: Preparation Of Ethanolic Extract Of Propolis (Eep)mentioning

confidence: 99%

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

Section: Mechanical Properties Instron Universal Testing Machine (Inmentioning

confidence: 99%

See 2 more Smart Citations

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Eskandarinia

Kefayat

Agheb

et al. 2020

Sci Rep

Self Cite

155

View full text Add to dashboard Cite

show abstract

“…The evaluation of potential cytotoxic effect displayed by the analysed formulations was tested using fibroblast and osteoblast reference cell cultures [27][28][29] (L929 and U2-OS, respectively) (ATCC, Manassas, VA, USA). Cells were incubated in a cell culture flask (Nunc, Roskilde, Denmark) at 37 • C/5% CO 2 in DMEM medium supplemented with 10% bovine serum (Sigma-Aldrich, Hamburg, Germany).…”

Section: Cytotoxicity Assay Of Mtz-loaded Matrices Toward Fibroblast mentioning

confidence: 99%

Metronidazole-Loaded Porous Matrices for Local Periodontitis Treatment: In Vitro Evaluation and In Vivo Pilot Study

et al. 2019

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Periodontal therapy focuses on thorough removal of subgingival calculus and plaque products followed by the smoothing out of root surfaces. However, such conventional mechanotherapeutic approaches are inefficient with regard to microbial biofilm elimination from the space between the root and deep periodontal pockets. Therefore, local chemotherapeutic agents need to be applied. Local antimicrobial treatment is also considered a safer treatment, as it avoids systemic complications related to drug application. In this study, porous matrices consisting of gelatin (GE) and cellulose derivatives (carboxymethylcellulose (CMC) and hydroxyethyl cellulose (HEC)) were loaded with antimicrobial drug metronidazole (MTZ). The matrices’ structural morphology, physiochemical properties, swelling and degradation ratio, mechanical properties, and MTZ release from the matrices were analyzed. Additionally, cytotoxicity tests for fibroblast and osteoblast cell cultures (L929 and U2-OS, respectively) and antimicrobial activity assessments of MTZ-loaded matrices against anaerobic Bacteroides sp. Bacteria were performed. Finally, clinical application of HEC matrices into periodontal pockets was conducted. The applied matrices showed a high antibacterial efficacy and a moderate cytotoxicity in vitro. The clinical application of HEC dressings corresponded with the decrease of periodontal pockets’ depth and bleeding observed 1 month after a single application. The presented results show that intra-pocket application of metronidazole using manufactured matrices may serve not only as a support for a standard treatment in periodontal practice but also as an alternative to systemic drug administration in this setting. Clinical data were analyzed using a nonparametric Friedman’s ANOVA for dependent trials.

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Biologically Enhanced Starch Bio‐Ink for Promoting 3D Cell Growth

Zhuang

Greenberg

2021

Adv Materials Technologies

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The excellent rheological property has legitimated the suitability of starch hydrogel for extrusion‐based 3D printing. However, the inability to promote cell attachment and migration has precluded the non‐modified starch hydrogel from direct applications in the biomedical field. Herein, a novel 3D printable nanocomposite starch hydrogel is developed with highly enhanced biocompatibility for promoting 3D cell growth, by formulating with gelatin nanoparticles and collagen. The rheological evaluation reveals the shear‐thinning and thixotropic properties of the starch‐based hydrogel, as well as the combinatorial effect of collagen and gelatin nanoparticles on maintaining printability and 3D shape fidelity. The homogeneous microporous structure with abundant collagen fibers and gelatin nanoparticles interlace and supplies rich attachment sites for cell growth. Corroborated by the cell metabolic activity study, the multiplied proliferation rate of cells on the 3D printed nanocomposite starch hydrogel scaffold confirms the remarkable enhancement of biological function of the developed starch hydrogel. Hence, the developed nanocomposite starch hydrogel serves as a highly desirable bio‐ink for advancing 3D tissue engineering.

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Cornstarch-based wound dressing incorporated with hyaluronic acid and propolis: In vitro and in vivo studies

Cited by 87 publications

References 63 publications

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

A Novel Bilayer Wound Dressing Composed of a Dense Polyurethane/Propolis Membrane and a Biodegradable Polycaprolactone/Gelatin Nanofibrous Scaffold

Metronidazole-Loaded Porous Matrices for Local Periodontitis Treatment: In Vitro Evaluation and In Vivo Pilot Study

Biologically Enhanced Starch Bio‐Ink for Promoting 3D Cell Growth

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