Cornus mas L. Increases Glucose Uptake and the Expression of PPARG in Insulin-Resistant Adipocytes

Abstract

Cornus mas L., also known as cornelian cherry (CM), is a species that has long been cultivated in many different countries. In numerous scientific reports, cornelian cherry is used to treat numerous diseases and conditions. The presented study evaluated the effect of red and yellow Cornus mas L. extract on insulin sensitivity in adipocytes. 3T3-L1 fibroblasts as well as human SAT-derived and VAT-derived adipocytes were differentiated in vitro, and insulin resistance was induced using palmitic acid (16:0). The effect of CM fruit extract was analyzed in terms of glucose uptake and insulin signaling gene expression. In the glucose uptake test after insulin stimulation, a significant increase in glucose uptake was demonstrated in cells treated with CM fruit extracts. Furthermore, CM fruit extracts increased the expression of insulin signaling genes in adipocytes stimulated with insulin in control cells and adipocytes treated with CM extract. Additionally, a significant increase in peroxisome proliferator activated receptor gamma (PPARG) expression was observed in cells supplemented with CM extract. In conclusion, studies have shown that CM fruits can overcome insulin resistance and thus they have a positive effect on cell metabolism.

Comparative Study of Cytotoxicity and Antioxidant, Anti-Aging and Antibacterial Properties of Unfermented and Fermented Extract of Cornus mas L.

“…In addition, dogwood is a rich source of minerals such as calcium, potassium, phosphorus, sodium, magnesium, iron, zinc and copper [21]. The composition of biologically active substances varies depending on the part of the plant [21,22].…”

An Evaluation of the Anti-Inflammatory Effects of a Thai Traditional Polyherbal Recipe TPDM6315 in LPS-Induced RAW264.7 Macrophages and TNF-α-Induced 3T3-L1 Adipocytes

“…In the present study, an upregulation of PPAR-γ was observed with the treatment with the TPDM6315 extracts (AE100 and EE10) and TGZ. These findings revealed a potential mechanism for TPDM6315, with a possible function of the PPAR-γ agonist that could be used to treat insulin resistance in type 2 diabetes [ 72 ].…”

Exploring the mechanism of Alisma orientale for the treatment of pregnancy induced hypertension and potential hepato-nephrotoxicity by using network pharmacology, network toxicology, molecular docking and molecular dynamics simulation

“…PPI network and GO/KEGG pathway enrichment analysis showed that AO toxic compounds mainly targeted PPARG and TNF and regulated the lipid and atherosclerosis signaling pathway. PPARG, also known as peroxisome proliferator-activated receptor gamma, is a nuclear receptor binding peroxisome proliferator that regulates adipocyte differentiation and controls the peroxisomal beta-oxidation pathway of fatty acids ( Małodobra-Mazur et al, 2022 ). Tumor necrosis factor (TNF) is mainly secreted by macrophages and can induce insulin resistance through inhibition of Insulin receptor substrate 1 (IRS1), tyrosine phosphorylation and G kinase-anchoring protein 1 (GKAP42) degradation in adipocytes ( Ando et al, 2015 ).…”