2019
DOI: 10.5395/rde.2019.44.e25
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Coronal tooth discoloration induced by regenerative endodontic treatment using different scaffolds and intracanal coronal barriers: a 6-month ex vivo study

Abstract: Objective The aim of this study was to evaluate discoloration of teeth undergoing regenerative endodontic procedures (REPs) using blood clot or platelet-rich fibrin (PRF) as the scaffolds and different calcium silicate-based materials as the intracanal coronal barriers in an ex vivo model. Materials and Methods Forty-eight bovine incisors were prepared and disinfected using 1 mg/mL double antibiotic paste (DAP). The specimens were then randoml… Show more

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Cited by 19 publications
(20 citation statements)
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“…The gelatinous consistency of the agar is similar to that of the dental pulp, making the insertion of the pulpotomy material closer to clinical practice (Oliveira et al, 2020). Bovine teeth were used instead of human teeth because they allow the selection of samples with more rigorous standards given the availability of samples, the greater flat surface for colour analysis and lack of concern about ethical issues (Yoldas et al, 2016, Shokouhinejad et al 2019, Oliveira et al, 2020).…”
Section: Discussionmentioning
confidence: 99%
“…The gelatinous consistency of the agar is similar to that of the dental pulp, making the insertion of the pulpotomy material closer to clinical practice (Oliveira et al, 2020). Bovine teeth were used instead of human teeth because they allow the selection of samples with more rigorous standards given the availability of samples, the greater flat surface for colour analysis and lack of concern about ethical issues (Yoldas et al, 2016, Shokouhinejad et al 2019, Oliveira et al, 2020).…”
Section: Discussionmentioning
confidence: 99%
“…4 ). It has been reported that sealing the dentinal tubules of the pulp chamber of bovine incisors with a dentin bonding agent, in a simulating regenerative endodontic treatment with the use of antibiotic and blood clot or platelet-rich fibrin, color change of coronal tooth structures filled with MTA or Biodentine was not different at 6 month follow-up [ 35 ].…”
Section: Discussionmentioning
confidence: 99%
“…The efficacy of internal bleaching already been proven in several in vitro and in vivo studies that included factors related to REP as etiologies of tooth discoloration, such as the use of MTA as a cervical barrier [30][31] , the use of triple antibiotic pastes 10,19 , or the presence of the blood clot 16 .…”
Section: Discussionmentioning
confidence: 99%
“…The most described MTA-related darkening mechanisms are heavy metal ion release and interaction of bismuth oxide with the collagen matrix, which will result in a darkened precipitate 30 ; interaction with the stimulated blood clot during the regenerative endodontic procedure 31 . However, studies have shown that internal bleaching is effective in reversing darkening caused by MTA 10,31 , as was observed in our study, where all cases showed clinically relevant change in color noticeable to the human eye (ΔE>3.7). Previous study observed that most of the discoloration in these cases are within the MTA and not in the dentin, so the removal of part of the MTA could improve the crown color in association with the internal bleaching 32 .…”
Section: Discussionmentioning
confidence: 99%
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