Coronary artery disease: differential expression of ceRNAs and interaction analyses

Kang, Sheng; Yong, Yan; Xia, Guang

doi:10.21037/atm-20-3487

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…We identified two core microRNAs (miR-466i-5p and miR-574-5p) in the microRNA-mRNA co-expression network that predicted the association between microRNA and target genes in MI + CIH heart tissue, and these were also associated with the PI3K-Akt signaling pathway. Indeed, several studies have shown that lncRNAs can act as ceRNAs that sequester miRNAs, thus allowing for de-repression of downstream miRNA targets (Wang et al, 2019;Kang et al, 2021). Such integration between coding and non-coding RNA forms complex ceRNA networks that when dysregulated lead to cardiac remodeling after MI (Wang et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, several studies have shown that lncRNAs can act as ceRNAs that sequester miRNAs, thus allowing for de-repression of downstream miRNA targets ( Wang et al, 2019 ; Kang et al, 2021 ). Such integration between coding and non-coding RNA forms complex ceRNA networks that when dysregulated lead to cardiac remodeling after MI( Wang et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

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lncRNA Mirt1: A Critical Regulatory Factor in Chronic Intermittent Hypoxia Exaggerated Post-MI Cardiac Remodeling

Xing

et al. 2022

Front. Genet.

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Chronic intermittent hypoxia (CIH) is the main feature of obstructive sleep apnea (OSA) and is known to exaggerate cardiac remodeling after myocardial infarction (MI). However, the specific contribution of CIH to overall OSA-induced pathological complications and the transcriptomic mechanisms underlying CIH-exaggerated post-MI remodeling remains unclear. In this study, we used RNA-sequencing to construct the expression profiles of cardiac mRNAs, microRNAs, and long non-coding RNAs (lncRNA) in four groups of C57BL/6J mice (Sham, CIH, MI, MI + CIH) to evaluate how CIH regulates cardiac remodeling after MI. Compared with the other three groups, the MI + CIH group exhibited 345 lncRNAs, 35 microRNAs, and 5,220 differentially expressed mRNAs. Further analysis showed that CIH led to significant changes in Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway of the differentially expressed mRNAs. Co-expression network analysis identified two core lncRNAs (Mirt1 and AC125351.1) and two core microRNAs (miR-466i-5p and miR-574-5p) during the development of CIH-exaggerated post-MI remodeling, and they were verified by quantitative real-time PCR (qRT-PCR). LncRNA-mRNA correlation analysis further showed that lncRNA Mirt1 was positively correlated with Apbb1ip and Lcp2. In addition, microRNA-mRNA correlation analysis showed that microRNA miR-466i-5p was positively correlated with Snai2, Cdc27, and Ngfr. Furthermore, combining with lncRNA-mRNA and miRNA-mRNA networks, 44 RNAs were identified in the competitive endogenous RNA (ceRNA) network. Mirt1 acts as a ceRNA to bind to miR-466i-5p to further regulate the expression levels of the target gene, thereby aggravating cardiac remodeling after MI. In conclusion, our study provides a systematic perspective on the potential functions of mRNAs, microRNAs, and lncRNAs in CIH-exaggerated post-MI cardiac remodeling. Our data suggest that lncRNA Mirt1 may be the most critical regulator of MI aggravated by CIH.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

lncRNA Mirt1: A Critical Regulatory Factor in Chronic Intermittent Hypoxia Exaggerated Post-MI Cardiac Remodeling

Xing

et al. 2022

Front. Genet.

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“…The overexpression of MEG3 decreased the expression of miR-145, which in turn increased the expression of the target gene PDCD4 and promoted cardiomyocyte apoptosis (5). Recently, despite a ceRNA literature report on CAD, it revealed 11 pathways and 15 key genes related to CAD, which provided options for the treatment of CAD (6). However, our study used different datasets from the previous ones.…”

Section: Introductionmentioning

confidence: 99%

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Zuo¹,

Xu²,

Wang³

et al. 2021

Ann Transl Med

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Background: The present study aimed to construct a network of competitive endogenous RNAs (ceRNAs) related to the pathogenesis of coronary artery disease (CAD), to provide a novel rationale for CAD treatment.Methods: Bioinformatics methods were applied to screen for differentially expressed long non-coding RNAs (DElncRNAs), microRNAs (DEmiRNAs), and mRNAs (DEmRNAs) from the GSE68506, GSE59421, and GSE20129 datasets of the Gene Expression Omnibus (GEO) database. The miRcode database was used to predict lncRNA-binding miRNAs. The miRTarBase, miRDB, and TargetScan databases were used to predict the target genes of these miRNAs. An mRNA-miRNA-lncRNA ceRNA network of CAD was established.Results: Between the CAD and normal control groups there were 264 DElncRNAs, 106 DEmiRNAs, and 1,879 DEmRNAs. We screened these differentially expressed gens (DEGs) respectively. There were 21 DElncRNAs, 13 DEmiRNAs, and 143 DEmRNAs in the ceRNA network by using Cytoscape application. The DEmRNAs were involved in the PI3K-Akt signaling pathway and the NF-κB signaling pathway. The key genes in the protein-protein interaction (PPI) network were HSP90AA1, CDKN1A,

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“…LncRNAs can regulate pathological mechanisms and disease progression through different target genes or signaling pathways, making them essential biomarkers [ 6 ]. LncRNAs, a non-coding RNA larger than 200 nucleotides, play a biological role in CAD by acting on downstream target molecules such as microRNAs (miRNAs, miRs), mRNAs, or transcription factors [ 7 , 8 ]. Most lncRNAs act as competing endogenous RNAs (ceRNAs) and regulate the expression and activation of downstream mRNAs by competitive binding with miRNAs, thus affecting the cardiovascular system’s biological functions, such as cell proliferation, migration, and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Role of LncRNAs in the Pathogenesis of Coronary Artery Disease

Wang¹,

Li²,

Sun³

et al. 2023

Rev. Cardiovasc. Med.

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Coronary artery disease (CAD), caused by coronary artery occlusion, is a common cardiovascular disease worldwide. Long non-coding RNAs (lncRNAs) are implicated in the regulation of endothelial cell injury, angiogenesis, plaque formation, and other pathological mechanisms in CAD by acting on different cell types. Some lncRNAs are significantly upregulated in CAD patients; however, other lncRNAs are significantly downregulated. Differential expression of lncRNAs in CAD patients enables them to be exploited as potential biomarkers to evaluate disease progression and diagnosis/prognosis in CAD patients. In this study, we reviewed the role of lncRNAs in the development of different clinical subtypes of CAD.

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Coronary artery disease: differential expression of ceRNAs and interaction analyses

Cited by 4 publications

References 48 publications

lncRNA Mirt1: A Critical Regulatory Factor in Chronic Intermittent Hypoxia Exaggerated Post-MI Cardiac Remodeling

lncRNA Mirt1: A Critical Regulatory Factor in Chronic Intermittent Hypoxia Exaggerated Post-MI Cardiac Remodeling

Role of competitive endogenous RNA networks in the pathogenesis of coronary artery disease

Role of LncRNAs in the Pathogenesis of Coronary Artery Disease

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