Coronil, a Tri-Herbal Formulation, Attenuates Spike-Protein-Mediated SARS-CoV-2 Viral Entry into Human Alveolar Epithelial Cells and Pro-Inflammatory Cytokines Production by Inhibiting Spike Protein-ACE-2 Interaction

Balkrishna, Acharya; Haldar, Swati; Singh, Harsh Vikram; Roy, Partha; Varshney, Anurag

doi:10.2147/jir.s298242

Cited by 35 publications

(30 citation statements)

References 63 publications

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“…It could hamper the interface of ACE-2 with SWT (wild-type S protein) and also with SD614G (more infectious variant). The elevated level of IL-6, IL-1β, and TNF-α in A549 cells also reduced after treatment with Coronil 96 . In another research investigation, Balkrishna et al, performed the analytical evaluation of Divya-Swasari-Vati, which is a herbal medication containing calcium prepared for providing relief from the respiratory symptoms due to corona infection.…”

Section: Recent Evidence Of Uses Of Herbs For Covid-19mentioning

confidence: 87%

Untitled

2021

IJPSR

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Coronavirus disease (COVID-19) has become pandemic in no time in the world. Understanding the pathophysiological similarities with previously known viruses of the class gave opportunities to explore various molecular targets against COVID-19. Vaccines unavailability propose different research approaches of treatment, including various previously known and some new synthetic molecules, which are also associated with many unwanted effects. Phytochemical compounds and formulations also gain interest in this era, and all the traditional medicine systems were analyzed for any probable therapeutic possibilities. Hence author summarize the various approaches of herbal-based medicine against COVID-19 infection. Ayurveda, Siddha, Unani, Homeopathy, and Chinese medicine systems constitute major traditional herbal-based systems known worldwide. Previous literature were studied to recapitulated findings with modern multi-mechanistic approaches and propose the use of herbals against COVID-19. Scientists are identifying all the possible interventions associated with herbal-based systems to have efficacy against various mechanisms against viral infection. Various herbal extracts and formulations are utilized to increase the human body's immune system to counter the pathophysiological changes due to COVID-19 infections. Promotion of these herbal-based medicines having a positive impact on the immune system is essential now. The review discussed the old and novel approaches revealing the efficacy of different phytoconstituents and herbal-based medicines against COVID-19. This review briefly highlighted the use of traditional herbal-based medicine systems against the novel coronavirus utilizing the modern approaches of multimechanistic targeting. A thorough investigation of different formulations from these systems is warranted through clinical studies for their global acceptance.

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Section: Recent Evidence Of Uses Of Herbs For Covid-19mentioning

confidence: 87%

Untitled

2021

IJPSR

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“…Observations from a randomized placebo controlled pilot clinical study, that evaluated the effectivity of these medicines on a slightly larger asymptomatic to mildly symptomatic patient pool, upholds our findings from this study ( Devpura et al, 2021 ). For better patient compliance, AC, GG and TG were combined and formulated into a tablet, called ‘Coronil’ and subsequently, its pharmacological effects against SARS-CoV-2 spike protein have been confirmed through in-vitro (( Balkrishna et al, 2021a ) and in-vivo ( Balkrishna et al, 2020c ) studies.…”

Section: Discussionmentioning

confidence: 99%

Comparative retrospective open-label study of ayurvedic medicines and their combination with allopathic drugs on asymptomatic and mildly-symptomatic COVID-19 patients

Balkrishna

Bhatt²,

Singh

et al. 2021

Journal of Herbal Medicine

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Introduction Treatment for COVID-19 was ambiguous in the beginning of the pandemic. At that time, the conventional medical system was grappling to cope with the rapidly spreading pandemic. The potential of Ayurveda, one of the branches of traditional Indian medicine (TIM), with a 5000 year old history, employing medicines derived from plants and other natural sources, against COVID-19 has been explored through a comparative retrospective open-label study. Methods Reported here are the remedial effects of Ayurvedic medicines alone or in combination with Allopathic treatment on 59 asymptomatic or mildly symptomatic COVID-19 patients, across multiple COVID-19 care centers in Ahmedabad, India. The patients were confirmed for COVID-19 infection through RT-qPCR of nasopharyngeal swabs. With informed consents from the patients, the sourced data was divided into ‘Allopathic and Ayurvedic’ [AlloAyur] (n = 41) and ‘Ayurvedic only’[Ayur] (n = 18) groups, based on the type of treatment the patients decided to receive, that is Ayurvedic medicines with Allopathic treatment or Ayurvedic medicines alone, respectively. Ayurvedic medicines included oral doses and nasal drops; the dosage and regime were decided based on the recommendations from Ayurvedic texts. The Allopathic medicines included Azithromycin, Vitamin-C and anti-histamines. Acetaminophen was also administered when necessary, by the attending physician. The patients were observed for symptomatic improvement. Results Primary outcome of this study was the symptomatic relief from COVID-19. Data collected over a period of two months, showed that more patients exhibited symptomatic relief in Ayur goup (83.33%) than in the AlloAyur group (48.78%) within the first 13 days of treatment. No visible adverse effects were observed. This indicated faster and safe symptomatic resolution among those treated with Ayurvedic medicines alone. Conclusion Patients receiving only Ayurvedic medicines on average were symptomatically relieved faster than those receiving Allopathic and Ayurvedic medicines together.

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“…Extract of Prunella vulgaris and our formulation share critical phytoconstituents, such as Quercetin, Ursolic acid, Saponins, Caffeic acids, Rosmarinic acid, Luteolin, Kaempferol, Gallic acid, etc. Furthermore, the study by Balkrishna et al, 2021, showed the herbal drug named Coronil effectively inhibited the interaction of ACE-2 with the wild-type S protein (SWT) and also prevented the SARS-CoV-2 S-protein pseudotyped vesicular stomatitis virus (VSVppSARS-2S) mediated cytokine response in these cells by reducing entry of pseudoviruses into host cells [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Indian Herb-Derived Phytoconstituent-Based Antiviral, Antimicrobial and Antifungal Formulation: An Oral Rinse Candidate for Oral Hygiene and the Potential Prevention of COVID-19 Outbreaks

Sharad

Kapila

2021

Pathogens

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Outbreaks of emerging infectious diseases continue to challenge human health. Novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has triggered a global coronavirus pandemic, known as COVID-19. Multiple variants of SARS-CoV-2 virus are circulating, thus raising questions with respect to the effectiveness of different lines of treatment, such as vaccines and antiviral drugs. To find the appropriate prevention/treatment, 21 plant-based ingredients (Glycyrrhizin, Withanone, Aloe-emodin, Rhein, Emodin, Chrysophanol, Physcion, Kaempferol, Progallin A, Gallic acid, Naringin, Quercetin, Luteolin, and Apigenin) having antiviral, antibacterial and antifungal properties were identified. We pseudo-typed SARS-CoV-2 on a lentiviral vector plasmid and tested the impact of five different herbal formulations in mammalian HEK293T cells. Viral inactivation assay showed that the natural extracts in a herb-derived phytoconstituent-based formulation, BITS-003, comprising Bacopa monnieri, Glycyerrhiza glabra, Asparagus racemosus-wild, and Nigella sativa had strong virucidal properties, inactivating enveloped viruses from 2log10 (or 99%) to >4log10 (or 99.99%). Moreover, bacterial and yeast cells treated with BITS-003 displayed reduced growth. Topical use of the formulation as a mouthwash/gargle could be effective in reducing symptoms of respiratory viral infections, with the potential to decrease the viral load in the buccal/oral cavity. This may inhibit the coronavirus spreading to the lungs of infected persons and at the same time may reduce the risk of viral transmission to other susceptible persons through micro-droplets originating from the oral cavity of the infected person.

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Coronil, a Tri-Herbal Formulation, Attenuates Spike-Protein-Mediated SARS-CoV-2 Viral Entry into Human Alveolar Epithelial Cells and Pro-Inflammatory Cytokines Production by Inhibiting Spike Protein-ACE-2 Interaction

Cited by 35 publications

References 63 publications

Untitled

Untitled

Comparative retrospective open-label study of ayurvedic medicines and their combination with allopathic drugs on asymptomatic and mildly-symptomatic COVID-19 patients

Indian Herb-Derived Phytoconstituent-Based Antiviral, Antimicrobial and Antifungal Formulation: An Oral Rinse Candidate for Oral Hygiene and the Potential Prevention of COVID-19 Outbreaks

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