Correction: Corrigendum: Identification of the phytosphingosine metabolic pathway leading to odd-numbered fatty acids

Catalytic residues, substrate specificity, and role in carbon starvation of the 2-hydroxy FA dioxygenase Mpo1 in yeast

“…In addition to the direct oxidation of FAs, 2-OH FA is also generated in the degradation pathway of the LCB phytosphingosine (PHS) (17,18). PHS is present in the epidermis, small intestine, and kidney in mammals (19)(20)(21) and is a major LCB in yeast (15,16).…”

“…PHS has a hydroxyl group at C4. LCBs undergo three common reactions in their degradation pathway: phosphorylation at C1, cleavage between the C2 and C3 positions, and oxidation (5,17,18,22,23). Through these reactions LCBs are converted to two-carbon shortened FAs, and PHS is converted to 2-OH FAs (17,18).…”

“…LCBs undergo three common reactions in their degradation pathway: phosphorylation at C1, cleavage between the C2 and C3 positions, and oxidation (5,17,18,22,23). Through these reactions LCBs are converted to two-carbon shortened FAs, and PHS is converted to 2-OH FAs (17,18). Because 2-OH FAs cannot be used for glycerolipid synthesis or -oxidation, they need to be converted to nonhydroxy FAs.…”

“…Because 2-OH FAs cannot be used for glycerolipid synthesis or -oxidation, they need to be converted to nonhydroxy FAs. This conversion is achieved by oxidation, where 2-OH FAs are metabolized to nonhydroxy FAs with one less carbon (making them odd-chain nonhydroxy FAs) (17,18). The -oxidation reactions differ between mammals and yeast.…”

“…The -oxidation reactions differ between mammals and yeast. Mammalian -oxidation involves three steps (CoA addition, long-chain aldehyde production by cleavage between the C1 and C2 positions, and oxidation to FAs), whereas yeast -oxidation is conducted in one step (17,18,24). Accordingly, the proteins involved in -oxidation differ between mammals and yeast.…”