Correction: Corrigendum: Rh D blood group conversion using transcription activator-like effector nucleases

Kim, Young-Hoon; Kim, Hyun O.; Baek, Eun Jung; Kurita, Ryo; Cha, Hyuk‐Jin; Nakamura, Yukio; Kim, Hyongbum

doi:10.1038/ncomms9678

Cited by 34 publications

(2 citation statements)

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“…As an example, we can consider the work of Kim Y.H. et al, who tried using the TALEN-based gene editor to change the set of antigens on the surface of erythroid progenitor cells, thus obtaining universal group I blood from the blood of any other group [ 20 ]. A nuclear localization signal was attached to the TALEN-encoding nucleotide sequence; on the whole, however, this vector can be called standard, except for one feature, i.e., it lacks a eukaryotic selective marker but this is due to the specificity and requirements of the experiment.…”

Section: Structural Elements Of Dna- and Rna-based Expression Vectors...mentioning

confidence: 99%

Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery

Zagoskin

Захарова²,

Nagornykh³

2022

Mol Biol

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Gene editing with programmable nucleases opens new perspectives in important practice areas, such as healthcare and agriculture. The most challenging problem for the safe and effective therapeutic use of gene editing technologies is the proper delivery and expression of gene editors in cells and tissues of different organisms. Virus-based and nonviral systems can be used for the successful delivery of gene editors. Here we have reviewed structural elements of nonviral DNA- and RNA-based expression vectors for gene editing and delivery methods in vitro and in vivo.

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Section: Structural Elements Of Dna- and Rna-based Expression Vectors...mentioning

confidence: 99%

Structural Elements of DNA and RNA Eukaryotic Expression Vectors for In Vitro and In Vivo Genome Editor Delivery

Zagoskin

Захарова²,

Nagornykh³

2022

Mol Biol

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“…In addition, the modular DNA-binding domain allows a simple rearranging of repeats to build designer TALEs with any desired DNA-binding specificity ( Cermak et al 2011 ; Geißler et al 2011 ; Weber et al 2011 ). Accordingly, TALEs have been employed as gene activators in synthetic biology ( Li et al 2015 ; Schreiber and Tissier 2017 ; Schreiber et al 2019 ) and started the genome editing revolution as TALE-nuclease fusions ( Christian et al 2010 ; Miller et al 2010 ; Li et al 2011 ; Kim et al 2015 ; Becker et al 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Designer TALEs enable discovery of cell death-inducer genes

Roeschlin,

Azad,

Grove

et al. 2024

Plant Physiology

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TALEs (transcription activator-like effectors) in plant-pathogenic Xanthomonas bacteria activate expression of plant genes and support infection or cause a resistance response. PthA4AT is a TALE with a particularly short DNA-binding domain harbouring only 7.5-repeats which triggers cell death in Nicotiana benthamiana; however, the genetic basis for this remains unknown. To identify possible target genes of PthA4AT that mediate cell death in N. benthamiana, we exploited the modularity of TALEs to stepwise enhance their specificity and reduce potential target sites. Substitutions of individual repeats suggested that PthA4AT-dependent cell death is sequence-specific. Stepwise addition of repeats to the C-terminal or N-terminal end of the repeat region narrowed the sequence requirements in promoters of target genes. Transcriptome profiling and in silico target prediction allowed the isolation of two cell death-inducer genes, which encode a patatin-like protein and a bifunctional monodehydroascorbate reductase/carbonic anhydrase protein. These two proteins are not linked to known TALE-dependent resistance genes. Our results show that the aberrant expression of different endogenous plant genes can cause a cell death reaction, which supports the hypothesis that TALE-dependent executor resistance genes can originate from various plant processes. Our strategy further demonstrates the use of TALEs to scan genomes for genes triggering cell death and other relevant phenotypes.

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