Correction: Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors

Barbosa, Graziela Murta; Colombo, A.; Rodrigues, Paulo Henrique Mazza; Simionato, Maria Regina Lorenzetti

doi:10.1371/journal.pone.0143903

Cited by 6 publications

(4 citation statements)

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“…Years after its discovery, infections caused by S. moorei have been seldom reported, and in these cases, the bacteria were mainly implicated in different types of oral diseases: periodontal diseases, periimplantitis [33], dentoalveolar abscesses [36], subgingival plaques from patients with refractory periodontitis [59], localized aggressive periodontitis [60], halitosis [22,64,65], endodontic infections [14,63], peri-radicular lesions [35], infected root canals [34]. As of now, there is insufficient evidence to suggest a role to S. moorei in the development of dental caries [20].…”

Section: Role Of S Moorei In Halitosismentioning

confidence: 99%

“…The participation of S. moorei in a biofilm fosters the presence of pigmented Prevotella and Porphyromonas species in the biofilm. This could be explained by the β-galactosidase activity of S. moorei, which would provide greater access to nutrients for proteolytic bacteria, such as P. gingivalis or P. intermedia [24,65]. Thus, S. moorei would facilitate integration of VSC-producing bacteria in the biofilm, but on the other hand, it is necessary to study the odor power of the VSCs that appeared in biofilms with S. moorei to establish how closely associated they are to bad breath [66].…”

Section: Role Of S Moorei In Halitosismentioning

confidence: 99%

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Small, but smelly: the importance of Solobacterium moorei in halitosis and other human infections

Barrak

Stájer

García-Lorenzana

et al. 2020

Heliyon

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Solobacterium moorei (S. moorei) has been described as Gram-positive, non spore forming, obligate anaerobic bacillus from human feces. The traditional culture and identification of these strains is very difficult (as the strains are often not cultivable or they grow only relatively slowly, in addition to producing only a very few positive biochemical reactions in commercially available identification kits); thus, reliable identification may only be carried out using methods, such as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and DNA sequencing. Regarding its pathogenic role, the relevance of S. moorei in halitosis (oral malodor) has a good standing, as it has been suggested by multiple studies, while the isolation of these bacteria from invasive infections is very rare; there are only a few reports available in the literature, regarding infections outside the oral cavity. Based on these reports, affected patients are predominantly characterized compromised immunity and are frequently associated with a dental focus of infection. The aim of our present review is to summarize the currently available knowledge on the pathogenic role of S. moorei in halitosis and other infections and to emphasize the relevance of this neglected anaerobic pathogen.

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Section: Role Of S Moorei In Halitosismentioning

confidence: 99%

Section: Role Of S Moorei In Halitosismentioning

confidence: 99%

Small, but smelly: the importance of Solobacterium moorei in halitosis and other human infections

Barrak

Stájer

García-Lorenzana

et al. 2020

Heliyon

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“…Moreover, besides proteinaceous components of the outer membrane, differences in LPS structures, mainly in lipid A, might be responsible for strains’ variability ( 89 , 101 – 104 ). It was reported that differences in both fimbriae and LPS between P. gingivalis strains can result in different hydrophobicity of strains, which may cause various recognition of molecules produced by cohabitating bacteria, different co-aggregation abilities, and different capacities for biofilm formation, and possibly differences in heme and hemoglobin binding by outer membrane molecules ( 103 – 108 ). Gingipains are modified by the APS ( 58 , 90 , 91 , 109 , 110 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Śmiga,

Ślęzak,

Olczak

2024

Microbiol Spectr

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Porphyromonas gingivalis strains exhibit different phenotypes in vitro , different virulence potential in animal models, and different associations with human diseases, with strains classified as virulent/more virulent (e.g., A7436 and W83) or as less virulent/avirulent (e.g., ATCC 33277). In this study, we comparatively analyzed the A7436 and ATCC 33277 strains to better understand their variability. Global gene expression analysis in response to heme and iron limitation revealed more pronounced differences in the A7436 than in the ATCC 33277 strain; however, in both strains, the largest changes were observed in genes encoding hypothetical proteins, genes whose products participate in energy metabolism, and in genes encoding proteins engaged in transport and binding proteins. Our results confirmed that variability between P. gingivalis strains is due to differences in the arrangement of their genomes. Analysis of gene expression of heme acquisition systems demonstrated that not only the availability of iron and heme in the external environment but also the ability to store iron intracellularly can influence the P. gingivalis phenotype. Therefore, we assume that differences in virulence potential may also be due to differences in the production of systems involved in iron and heme acquisition, mainly the Hmu system. In addition, our study showed that hemoglobin, in a concentration-dependent manner, differentially influences the virulence potential of P. gingivalis strains. We conclude that iron and heme homeostasis may add to the variability observed between P. gingivalis strains. IMPORTANCE Periodontitis belongs to a group of multifactorial diseases, characterized by inflammation and destruction of tooth-supporting tissues. P. gingivalis is one of the most important microbial factors involved in the initiation and progression of periodontitis. To survive in the host, the bacterium must acquire heme as a source of iron and protoporphyrin IX. P. gingivalis strains respond differently to changing iron and heme concentrations, which may be due to differences in the expression of systems involved in iron and heme acquisition. The ability to accumulate iron intracellularly, being different in more and less virulent P. gingivalis strains, may influence their phenotypes, production of virulence factors (including proteins engaged in heme acquisition), and virulence potential of this bacterium.

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“…Outros estudos adicionam ao consórcio microbiano para indução da doença periodontal experimental também P. intermedia (Blasco-Baque et al, 2017). Em estudo recente, a infecção oral experimental por P. gingivalis fimbriada associada a um isolado clínico de P.intermedia com alta capacidade de agregação a P. gingivalis (Barbosa et al, 2015) induziu maior destruição periodontal em camundongos C57Bl6 do que quando um dos agentes era inoculado separadamente (Simionato, dados não publicados). Além de agregar com P. intermedia (Barbosa et al, 2015), P. intermedia colabora com o estabelecimento da disbiose oral promovida por P. gingivalis pois a interpaína A, produzida por P. intermedia, também degrada fatores do complemento (Potempa et al,2009) e captura o fator H do complemento, um fator fisiológico inibidor do complemento (Malm et al,2012).…”

Section: Modelos Experimentais De Periodontiteunclassified

Efeito do probiótico Lactobacillus acidophilus LA5 em modelo de periodontite induzida em camundongos diabéticos

Cardoso¹

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Agradeço primeiramente a Deus por ter me concedido a honra de realizar este trabalho e de tÊ-lo comigo em todos os momentos, sendo a minha fortaleza.Agradeço ao meu esposo Augusto Cesar Santos SIlva, meu melhor amigo, meu parceiro, meu presente de Deus, que me deu forças, incentivo, motivação e que acima de tudo orou por mim todos os dias para que eu pudesse chegar ao final com êxito e alegria.Agradeço aos meus pais e ao meu irmão, pela base e pela estrutura familiar que sempre me deram, pelo amparo, pelo amor incondicional e também por suas orações me fazendo sempre acreditar que os sonhos se realizam e que as promessas de Deus se cumprem na vida daqueles que O amam. Agradeço aos meus sogros, pelas orações, pelas palavras amigas e por me fazerem sempre lembrar que somos servos de Deus e Ele nunca nos desampara. Agradeço a Universidade de São Paulo, ao Instituto de Ciências Biomédicas e ao departamento de Microbiologia, sempre com admiração e respeito. Agradeço a minha orientadora Profa. Dra. Marcia Pinto Alves Mayer, pelo empenho e dedicação em me orientar, pela paciência e pelo amparo em todas as fases do projeto e também da vida nesses anos compartilhados, pelo auxílio, pela amizade e por ter acreditado em mim. Um exemplo de professora, de ser humano, de excelência em tudo que se propõe a fazer. Foi uma honra ser sua orientada. Agradeço a Dra. Pamela Pontes Amado, pela grande amizade que construímos e por ter sido meu primeiro exemplo dentro do laboratório, me inspirando e me ensinando com amor a dar os meus primeiros passos dentro do laboratório. Meu amor, meu respeito e minha admiração. Agradeço a todos os professores e colegas do departamento de Microbiologia. O presente trabalho foi realizado com apoio da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior -Brasil (CAPES) -Código de Financiamento 001. regulada positivamente quando D+PPPB foi comparado ao controle D+PP (ANOVA, p<0.05)., L.acidophilus LA5 induziu a regulação positiva da transcrição de ocln, tanto na presença como na ausência de periodontite, embora não tenha sido observada diferença significante na expressão deste gene entre os grupos D+PP e D+PPPB. A periodontite e/ou a diabetes não interferiram na expressão relativa de muc1, no entanto, a administração do probiótico levou a regulação positiva de muc1 (não significante) quando comparados os grupos D+SHAM e D+PB, e esta regulação positiva pelo probiótico foi estatisticamente significante entre os grupos D+SHAM e D+PP e D+PPPB. Apesar das limitações dos modelos animais, o presente estudo sugere que L. acidophilus LA5 apresenta potencial para ser utilizado como adjuvante ao tratamento da diabetes tipo 2 e da periodontite. No entanto, outros estudos experimentais elucidando seu mecanismo de ação devem ser realizados antes de seu uso clínico. Palavras chaves: Periodontite. Diabetes. Lactobacillus acidophilus. Probiótico.

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Correction: Intraspecies Variability Affects Heterotypic Biofilms of Porphyromonas gingivalis and Prevotella intermedia: Evidences of Strain-Dependence Biofilm Modulation by Physical Contact and by Released Soluble Factors

Abstract: The image for Fig 3 is missing Fig 3B and 3C. Please see the corrected Fig 3 here. Table 1 has been corrected for improved readability. Please see the corrected Table 1 here.

Cited by 6 publications

References 1 publication

Small, but smelly: the importance of Solobacterium moorei in halitosis and other human infections

Small, but smelly: the importance of Solobacterium moorei in halitosis and other human infections

Comparative analysis of Porphyromonas gingivalis A7436 and ATCC 33277 strains reveals differences in the expression of heme acquisition systems

Efeito do probiótico Lactobacillus acidophilus LA5 em modelo de periodontite induzida em camundongos diabéticos

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