Correction: Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients

Santonastaso, Marianna; Pucciarelli, Alessia; Costantini, Susan; Caprio, Francesca; Sorice, Angela; Capone, Francesca; Antonella, Natella; Iardino, Patrizia; Colacurci, Nicola; Chiosi, Emilio

doi:10.1039/c7mb90021j

Cited by 15 publications

(10 citation statements)

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“…The results showed an increase in β-hydroxybutyric acid and glutamine and a decrease in tryptophan as well as an alteration of pathways such as nitrogen metabolism, pyrimidine metabolism, glutamine and glutamate metabolism, and aminoacyl-tRNA biosynthesis. Endometriosis can be considered an inflammatory disease with evidence of elevated levels of peritoneal fluid cytokines and growth factors, alterations in B cell activity, and an increased incidence of autoantibodies [18,29]. Furthermore, peritoneal macrophages are increased in number, concentration, and activity in women with the disease.…”

Section: Discussionmentioning

confidence: 99%

Metabolic Profile of Patients with Severe Endometriosis: a Prospective Experimental Study

et al. 2020

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Endometriosis is a common disease affecting women in reproductive age. There are several hypotheses on the pathogenesis of this disease. Often, its lesions and symptoms overlap with those of many other medical and surgical conditions, causing a delay in diagnosis. Metabolomics represents a useful diagnostic tool for the study of metabolic changes during a different physiological or pathological status. We used 1H-NMR to explore metabolic alteration in a cohort of patients with endometriosis in order to contribute to a better understanding of the pathophysiology of the disease and to suggest new useful biomarkers. Thirty-seven patients were recruited for the metabolomic analysis: 22 patients affected by symptomatic endometriosis and 15 not affected by it. Their serum samples were collected and analyzed with 1H-NMR. Multivariate statistical analysis was conducted, followed by univariate and pathway analyses. Partial Least Square Discriminant Analysis (PLS-DA) was performed to determine the presence of any differences between the non-endometriosis and endometriosis samples (R2X = 0.596, R2Y = 0.713, Q2 = 0.635, and p < 0.0001). β-hydroxybutyric acid and glutamine were significantly increased, whereas tryptophan was significantly decreased in the endometriosis patients. ROC curves were built to test the diagnostic power of the metabolites (β-hydroxybutyric acid: AUC = 0.85 CI = 0.71–0.99; glutamine: AUC = 0.83 CI = 0.68–0.98; tryptophan: AUC = 0.75 CI = 0.54–0.95; β-hydroxybutyric acid + glutamine + tryptophan AUC = 0.92 CI = 0.81–1). The metabolomic approach enabled the identification of several metabolic alterations occurring in women with endometriosis. These findings may provide new bases for a better understanding of the pathophysiological mechanisms of the disease and for the discovery of new biomarkers. Trial registration number NCT02337816

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Section: Discussionmentioning

confidence: 99%

Metabolic Profile of Patients with Severe Endometriosis: a Prospective Experimental Study

et al. 2020

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“…In the last years, FF has been investigated as an ideal candidate for non-invasive method of oocyte selection in fertility clinics [29]. Metabolic profiling of FF has been applied to find biomarkers of oocyte developmental competence [30] and to find also specific panels of biomarkers related to some infertility pathologies [31][32][33][34][35].…”

Section: Discussionmentioning

confidence: 99%

NMR metabolomics study of follicular fluid in women with cancer resorting to fertility preservation

Morelli

Iuliano

Schettini

et al. 2018

J Assist Reprod Genet

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“…In recent years, several studies have been carried out to determine the metabolic FF profile of women with infertility due to different causes. Using an untargeted metabolomic ap-proach, with the aid of high-resolution proton nuclear magnetic resonance ( 1 H-NMR) [9,10] or liquid/gas chromatography-mass spectrometry [5,11], it has been shown that alteration of some metabolites in the FF of infertile females (IF) were affected by PCOS [12], ROR [13] and EM [14,15]. Some metabolomic studies have even found significant differences in IF with negative IVF outcomes compared to IF with positive IVF outcomes [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

Lazzarino

Pallisco²,

Bilotta³

et al. 2021

IJMS

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Nearly 40–50% of infertility problems are estimated to be of female origin. Previous studies dedicated to the analysis of metabolites in follicular fluid (FF) produced contrasting results, although some valuable indexes capable to discriminate control groups (CTRL) from infertile females (IF) and correlate with outcome measures of assisted reproduction techniques were in some instances found. In this study, we analyzed in blind FF of 35 control subjects (CTRL = patients in which inability to obtain pregnancy was exclusively due to a male factor) and 145 IF (affected by: endometriosis, n = 19; polycystic ovary syndrome, n = 14; age-related reduced ovarian reserve, n = 58; reduced ovarian reserve, n = 29; unexplained infertility, n = 14; genetic infertility, n = 11) to determine concentrations of 55 water- and fat-soluble low molecular weight compounds (antioxidants, oxidative/nitrosative stress-related compounds, purines, pyrimidines, energy-related metabolites, and amino acids). Results evidenced that 27/55 of them had significantly different values in IF with respect to those measured in CTRL. The metabolic pattern of these potential biomarkers of infertility was cumulated (in both CTRL and IF) into a Biomarker Score index (incorporating the metabolic anomalies of FF), that fully discriminated CTRL (mean Biomarker Score value = 4.00 ± 2.30) from IF (mean Biomarker Score value = 14.88 ± 3.09, p < 0.001). The Biomarker Score values were significantly higher than those of CTRL in each of the six subgroups of IF. Posterior probability curves and ROC curve indicated that values of the Biomarker Score clustered CTRL and IF into two distinct groups, based on the individual FF metabolic profile. Furthermore, Biomarker Score values correlated with outcome measures of ovarian stimulation, in vitro fertilization, number and quality of blastocysts, clinical pregnancy, and healthy offspring. These results strongly suggest that the biochemical quality of FF deeply influences not only the effectiveness of IVF procedures but also the following embryonic development up to healthy newborns. The targeted metabolomic analysis of FF (using empowered Redox Energy Test) and the subsequent calculation of the Biomarker Score evidenced a set of 27 low molecular weight infertility biomarkers potentially useful in the laboratory managing of female infertility and to predict the success of assisted reproduction techniques.

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Correction: Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients

Abstract: Correction for ‘Metabolomic profiling and biochemical evaluation of the follicular fluid of endometriosis patients’ by Marianna Santonastaso et al., Mol. BioSyst., 2017, DOI: 10.1039/c7mb00181a.

Cited by 15 publications

References 0 publications

Metabolic Profile of Patients with Severe Endometriosis: a Prospective Experimental Study

Metabolic Profile of Patients with Severe Endometriosis: a Prospective Experimental Study

NMR metabolomics study of follicular fluid in women with cancer resorting to fertility preservation

Altered Follicular Fluid Metabolic Pattern Correlates with Female Infertility and Outcome Measures of In Vitro Fertilization

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