Correction of the functional state of female rats after unilateral ovariectomy using a succinate containing composition

Vasilieva, A. A.; Simonova, Maria; Bairamov, A.A.; Grishina, E. V.; Uchitel, M. L.; Maevsky, Eugene I.

doi:10.12710/cardiometry.2017.8692

Cited by 4 publications

(2 citation statements)

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“…Новым подходом в профилактике и лечении сенильного и постменопаузального остеопороза и дефицита витамина Д 3, в частности, является применение в лекарственной терапии комплекса кислых солей янтарной кислоты, потенциально влияющего на усвоение макро-и микроэлементов костной тканью, на биотрансформацию витамина Д 3 и повышающего биодоступность его активных форм в организме [12, [16][17][18][19]. Из природных субстратов-метаболитов кислые соли сукцината являются наиболее сильными модуляторами орфанных рецепторов, кальциевых каналов L-типа, активируют аккумуляции Са 2+ внутри клетки эндоплазматическим и саркоплазматическим ретикулумом и митохондриями, активируют лимитирующий этап в метаболизме холестерола -вход в митохондрий и последующую биотрансформацию в активные формы стероидов [16,18,20,21]. В экспериментальном исследовании сукцинат-содержащий комплексный препарат повышал индекс массы костной ткани, увеличивал синтез эстрогенов и андрогенов в условиях гормональ-ного дефицита [17,19,22].…”

Section: Introductionunclassified

Morphological and biochemical characteristics of osteogenesis during drug therapy of experimental osteoporosis

Lisovsky,

Mamina,

Droblenkov

et al. 2023

Transl. med. (Print)

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Relevance. Osteoporosis is a clinical and economic problem on a global scale. A significant contribution to solving the problem of effective treatment of osteoporosis can be the creation of drugs based on unique biologically active compounds.The aim of the study was the morphological substantiation of the experimental model of osteoporosis and a comprehensive (multifactorial) assessment of the effectiveness of its drug therapy.Materials and methods. The study was carried out on 40 mature female Wistar rats, which comprised 6 groups. A model of osteoporosis was formed in animals of four experimental groups (6 animals in each): the first and second groups (respectively) consisted of young rats, the third and fourth — old ones. Animals of the second and fourth groups were injected with a drug tested for its ability to activate the process of osteosynthesis. Rats of the fifth and sixth groups (young and old, 8 animals each), subjected to sham surgery, served as controls. As a result of the use of the histo-morphometric method and atomic absorption spectroscopy in the diaphysis of the femur, the thickness of the layers of the compact substance, the number of bone plates and osteocytes, as well as the amount of collagen, calcium and phosphorus were determined. Using enzyme immunoassay, bone remodeling markers — osteocalcin, sclerostin, osteoprotegerin, fibroblast growth factor-23 and nuclear factor kappa-β activator ligand (RANKL) — were determined in the blood serum. Statistical processing of the data was carried out using the GraphPad PRISM (USA) program to determine the median, upper and lower quartiles. Differences were considered significant at p < 0.01.Results. Modeling of osteoporosis induces atrophic thinning of the compact substance, a decrease in the number of osteocytes and bone plates in the diaphysis of the femur, a decrease in the content of collagen, calcium and phosphorus in them, a decrease in the concentration of osteocalcin, sclerostin, fibroblast growth factor, osteoprotegerin and an increase in the concentration of RANKL in the blood plasma, more pronounced in old animals. As a result of the use of the drug X3 for the treatment of osteoporosis, the following were revealed: a significant increase in the thickness of the compact substance, the number of osteocytes and bone plates in the diaphysis, the content of collagen, calcium and phosphorus in them, an increase in the concentration of biochemical markers of osteosynthesis, and a mild imbalance of RANKL. The increase in plasma levels of markers of bone remodeling was most pronounced when the drug X3 was combined with vitamin D3.Conclusion. 1. The used surgical-endocrine method of modeling osteoporosis leads to pronounced degenerative changes in osteocytes and their derivatives in all parts of the compact bone substance, and also causes significant disturbances in the mineral composition and an imbalance of bone remodeling markers, more pronounced in senile rats. 2. The tested drug X3, used for the treatment of osteoporosis, has a high degree of effectiveness, since it stimulates regenerative osteogenesis, restores the damaged structure of bone tissue elements, its organic and mineral components. 3. The restorative effect of the drug is more pronounced in senile rats.

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Section: Introductionunclassified

Morphological and biochemical characteristics of osteogenesis during drug therapy of experimental osteoporosis

Lisovsky,

Mamina,

Droblenkov

et al. 2023

Transl. med. (Print)

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“…Остеопороз -это системное метаболическое заболевание скелета, характеризующееся снижением массы костей и нарушениями микроархитектоники костной ткани, которое приводит к значительному увеличению хрупкости костей и возможности их переломов [29]. По данным Европейского фонда остеопороза (International Osteoporosis Foundation, или IOF (2010)), ОП страдают около 75 млн человек в Европе, США и Японии [22,23,28].…”

Section: Introductionunclassified

Correction of bone remodeling in experimental osteoporosis

Bairamov

Азизович²,

Maevsky

et al. 2020

Rev Clin Pharm Drug Ther

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The experimental work analyzes the evaluation of the pharmacological efficacy of 3 experimental samples of the anti-osteoporosic preparation, created on the basis of salts of succinic acid in comparison with the comparison drug. On the experimental model of osteoporosis, according to the application of atomic absorption spectroscopy and flame photometry of the femur, the high efficiency of the new drug in the correction of induced pathology has been proved. The data obtained indicate that when acid salts of the natural conformer of succinic acid are used, it is possible to achieve sufficient saturation of bone tissue with calcium without overloading the body with excess calcium.

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Evaluation of osteogenesis processes against the background of experimental osteoporosis therapy

Bayramov,

Mamina,

Lisovskiy

et al. 2023

Rev Clin Pharm Drug Ther

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Background. Osteoporosis is a problem all over the world with important clinical and economic consequences. A significant contribution to solving the problem of the spread of osteoporosis can be the creation of drugs based on unique biologically active compounds. The aim was to evaluate the processes of osteogenesis, according to the formation of an organic matrix of bone tissue, as well as to evaluate markers of bone remodeling in blood serum at the stages of anti-osteoporosis therapy. Materials and methods. The study was performed on an experimental model of osteoporosis using biochemical methods for analyzing markers of osteoporosis in blood serum, as well as atomic absorption spectroscopy and X-ray densitometry. Results. According to the results of the study, the specific anti-osteoporotic activity of the new drug based on succinic acid salts was proved - a significant increase in the organic component the total collagen in bone tissue and the mineral component - the main elements in bone tissue in both young and old senile animals. Evaluation of the dynamics of the content of markers of bone remodeling showed the high effectiveness of the new drug in monotherapy, and in combination with vitamin D3 in the activation of osteogenesis processes in experimental osteoporosis. Conclusion. The effectiveness of the proposed anti-osteoporotic agent is shown, which is more pronounced in senile rats and is due to a proportional increase in the organic and mineral components of bone tissue.

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Correction of the functional state of female rats after unilateral ovariectomy using a succinate containing composition

Cited by 4 publications

References 3 publications

Morphological and biochemical characteristics of osteogenesis during drug therapy of experimental osteoporosis

Morphological and biochemical characteristics of osteogenesis during drug therapy of experimental osteoporosis

Correction of bone remodeling in experimental osteoporosis

Evaluation of osteogenesis processes against the background of experimental osteoporosis therapy

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