Correction pen based paper fluidic device for the detection of multiple gene targets of Leptospira using Loop Mediated Isothermal Amplification

Varsha, Venkatesh; Aishwarya, S.; Murchana, Sarma; Naveen, Gattuboyena; Ramya, Mohandass; Rathinasabapathi, Pasupathi

doi:10.1016/j.mimet.2020.105962

Cited by 12 publications

(10 citation statements)

References 20 publications

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“…A study from Varsha et al, targeting Leptospira spp., used 62.5 M of LCV after LAMP amplification. This increase in concentration may be related with the fact that the amplification reaction was run in a paper-based device, thus higher LCV concentration may be needed for clear color differentiation (Varsha et al, 2020). Roy et al reported the usage of this dye for the detection of Bacillus subtilis in a paper microchip (Roy et al, 2017), and more recently it has been applied in a study dealing with the detection of SARS-CoV-2, expanding the application of a previous study combining RPA and LAMP, that is, RAMP (El-Tholoth et al, 2021;Song, Liu, et al, 2017).…”

Section: Crystal Violetmentioning

confidence: 99%

“…A study from Varsha et al., targeting Leptospira spp., used 62.5 M of LCV after LAMP amplification. This increase in concentration may be related with the fact that the amplification reaction was run in a paper‐based device, thus higher LCV concentration may be needed for clear color differentiation (Varsha et al., 2020). Roy et al.…”

Section: Naked‐eye Detectionmentioning

confidence: 99%

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Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Garrido‐Maestu

Prado

2022

Comp Rev Food Sci Food Safe

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Nucleic acid amplification‐based techniques have gained acceptance by the scientific, and general, community as reference methodologies for many different applications. Since the development of the gold standard of these techniques, polymerase chain reaction (PCR), back in the 1980s many improvements have been made, and alternative techniques emerged reporting improvements over PCR. Among these, isothermal amplification approaches resulted of particular interest as could overcome the need of specialized equipment to accurately control temperature changes, but it was after year 2000 that these techniques have flourished in a huge number of novel alternatives with many different degrees of complexities and requirements. An added value is their possibility to be combined with many different naked‐eye detection strategies, simplifying the resources needed, allowing to reduce cost, and serving as the basis for novel developments of lab‐on‐chip systems, and miniaturized devices, for point‐of‐care testing. In this review, we will go over different types of naked‐eye detection strategies, combined with isothermal amplification. This will provide the readers up‐to‐date information for them to select the most appropriate strategies depending on the particular needs and resources for their experimental setup.

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Section: Crystal Violetmentioning

confidence: 99%

Section: Naked‐eye Detectionmentioning

confidence: 99%

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Garrido‐Maestu

Prado

2022

Comp Rev Food Sci Food Safe

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“…absorbance) (excitation/emission wavelengths [k ex/em ] ¼ 540 nm/610 nm) allowing detection of 10 2 to 10 5 DNA copies. Similarly, Varsha et al (2020) describe an even-simpler paper device for Leptospira DNA using commercial Whatman (Maidstone, England) no. 1 paper, again with dried LAMP reagents detected via smartphone.…”

Section: Paper-based Microfluidic Devicesmentioning

confidence: 99%

“…A reliable supply chain and storage capacity are essential in all settings but are particularly an issue in regions with poorly developed infrastructure, notably those lacking cold chain or storage (28C to 88C or À208C). Dried and/or lyophilized reagents are components of several cartridge-based systems (TwistDx [Abbot Laboratories, Abbott Park, IL] for RPA, Simprova [Eiken] for LAMP, GenExpert [Cepheid] for PCR) and also components of some paper-based microfluidic devices (see above, Seok et al 2017, Varsha et al 2020, Garneret et al 2021. Others have sought to prepare lyophilized (freeze-dried) reagents as standalone reagents with the objective of stabilizing and prolonging room-temperature shelf-life (Pack et al 2008, Challener 2017, Chen and Ching 2017.…”

Section: Lyophilization and Dry Storage Of Rt-lamp Reagentsmentioning

confidence: 99%

Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications

Moore

Cahill²,

Aidelberg

et al. 2021

J Biomol Tech

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As the second year of the COVID-19 pandemic begins, it remains clear that a massive increase in the ability to test for SARS-CoV-2 infections in a myriad of settings is critical to controlling the pandemic and to preparing for future outbreaks. The current gold standard for molecular diagnostics is the polymerase chain reaction (PCR), but the extraordinary and unmet demand for testing in a variety of environments means that both complementary and supplementary testing solutions are still needed. This review highlights the role that loop-mediated isothermal amplification (LAMP) has had in filling this global testing need, providing a faster and easier means of testing, and what it can do for future applications, pathogens, and the preparation for future outbreaks. This review describes the current state of the art for research of LAMP-based SARS-CoV-2 testing, as well as its implications for other pathogens and testing. The authors represent the global LAMP (gLAMP) Consortium, an international research collective, which has regularly met to share their experiences on LAMP deployment and best practices; sections are devoted to all aspects of LAMP testing, including preanalytic sample processing, target amplification, and amplicon detection, then the hardware and software required for deployment are discussed, and finally, a summary of the current regulatory landscape is provided. Included as well are a series of first-person accounts of LAMP method development and deployment. The final discussion section provides the reader with a distillation of the most validated testing methods and their paths to implementation. This review also aims to provide practical information and insight for a range of audiences: for a research audience, to help accelerate research through sharing of best practices; for an implementation audience, to help get testing up and running quickly; and for a public health, clinical, and policy audience, to help convey the breadth of the effect that LAMP methods have to offer.

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“…In order to create hydrophobic patterns on paper, several materials and PoP methodologies have been applied: commercial wax pencils or crayons [19][20][21]; a heated pen to dispense molten wax [22]; commercial acrylate-based plastic [23]; typo correction pens [24,25]; and 3D pens [26]. The disadvantages of these methods are the limited spatial resolution achieved and the need for post-fabrication treatment (by heating or UV irradiation).…”

Section: Introductionmentioning

confidence: 99%

Development of a High-Throughput Low-Cost Approach for Fabricating Fully Drawn Paper-Based Analytical Devices Using Commercial Writing Tools

et al. 2021

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This work reports the development and optimization of a rapid and low-cost pen-on-paper plotting approach for the fabrication of paper-based analytical devices (PADs) using commercial writing stationery. The desired fluidic patterns were drawn on the paper substrate with commercial marker pens using an inexpensive computer-controlled x–y plotter. For the fabrication of electrochemical PADs, electrodes were further deposited on the devices using a second x–y plotting step with commercial writing pencils. The effect of the fabrication parameters (type of paper, type of marker pen, type of pencil, plotting speed, number of passes, single- vs. double-sided plotting), the chemical resistance of the plotted devices to different solvents and the structural rigidity to multiple loading cycles were assessed. The analytical utility of these devices is demonstrated through application in optical sensing of total phenols using reflectance calorimetry and in electrochemical sensing of paracetamol and ascorbic acid. The proposed manufacturing approach is simple, low cost, flexible, rapid and fit-for-purpose and enables the fabrication of sub-“one-dollar” PADs with satisfactory mechanical and chemical resistance and good analytical performance.

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Correction pen based paper fluidic device for the detection of multiple gene targets of Leptospira using Loop Mediated Isothermal Amplification

Cited by 12 publications

References 20 publications

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Naked‐eye detection strategies coupled with isothermal nucleic acid amplification techniques for the detection of human pathogens

Loop-Mediated Isothermal Amplification Detection of SARS-CoV-2 and Myriad Other Applications

Development of a High-Throughput Low-Cost Approach for Fabricating Fully Drawn Paper-Based Analytical Devices Using Commercial Writing Tools

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