Correction: Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition in Pseudomonas aeruginosa via LasIR/RhlIR circuitry

Abstract

Correction for ‘Potassium 2-methoxy-4-vinylphenolate: a novel hit exhibiting quorum-sensing inhibition in Pseudomonas aeruginosa via LasIR/RhlIR circuitry’ by Mayank D. Shah et al., RSC Adv., 2019, 9, 40228–40239.

Effects of active compounds from Cassia fistula on quorum sensing mediated virulence and biofilm formation in Pseudomonas aeruginosa

“…The melting curve was done as follows: analysis 60 °C to 95 °C, with a rise in temperature of 0.5 °C every 3 s. The housekeeping rpsL gene was employed as a reference for normalization of gene expression; the 2 −ΔΔ C t method was utilised to determine the differential gene expression of the QS-associated genes. 41…”

“…Three independent assays in triplicate were performed (n ¼ 9). 41 2.9 Estimation of the extracellular virulence factors 2.9.1 Supernatant preparation. Overnight grown P. aeruginosa was inoculated in the appropriate medium and grown till OD reached 0.2 at 600 nm, and this culture was used for all the assays.…”

“…The method previously described by Kessler et al & Shah et al 41,42 was employed for staphylolytic protease quantication. 500 ml culture supernatants of F-1, rhein & AZM were suspended in an equal volume of overnight grown boiled S. aureus suspension (prepared in 0.03 M Tris-Cl buffer (pH $ 8.5)).…”

“…Confocal laser scanning microscopy (CLSM) investigation of the test sample treated P. aeruginosa was done as reported previously by Singh et al and Shah et al 41,52 For the CLSM analysis, the treated bacterial samples were prepared and incubated the same way as stated for SEM analysis. Following the incubation, slides were carefully washed with phosphate buffer saline (pH $ 7.2) to detach unadhered bacterial cells, stained with a mixture of SYTO9/propidium iodide, and placed in the dark (15 min).…”

“…The melting curve was done as follows: analysis 60 C to 95 C, with a rise in temperature of 0.5 C every 3 s. The housekeeping rpsL gene was employed as a reference for normalization of gene expression; the 2 ÀDDC t method was utilised to determine the differential gene expression of the QS-associated genes. 41 2.13 Effect of test samples on survival of Caenorhabditis elegans (C. elegans) 2.13.1 C. elegans paralytic assay. C. elegans culture was maintained as mentioned in ESI (Method 1 †).…”