Correction to: Breast cancer cell motility is promoted by 14-3-3γ

Hiraoka, Emiko; Mimae, Takahiro; Ito, Masaoki; Kadoya, Takayuki; Miyata, Yoshihiro; Ito, Akihiko; Okada, Minoru

doi:10.1007/s12282-019-00964-5

Cited by 3 publications

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“…Consequently, it is causally related with migration and invasion of tumor cells and the presence of metastases. Similarly, to our study, this isoform has already been linked to metastases of breast tumors and its inhibition has reduced the invasiveness of malignant cells [49]. In any case, regardless of the isoform, inhibition of 14-3-3 protein is strongly linked to inhibition of cell proliferation and migration, in addition to stimulating apoptosis [46][47][48].…”

Section: Discussionsupporting

confidence: 83%

“…The 14-3-3 protein has ability to bind to oncogenic proteins and therefore its overexpression is highly related to several tumor types, such as breast, prostate, ovaries and lung cancer [47,48]. 14-3-3γ, found in this study, is one of the isoforms already identified in 14-3-3 protein, and is related to cell motility [48,49]. Consequently, it is causally related with migration and invasion of tumor cells and the presence of metastases.…”

Section: Discussionmentioning

confidence: 52%

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Proteomics Approach of Rapamycin Anti-Tumoral Effect on Primary and Metastatic Canine Mammary Tumor Cells In Vitro

et al. 2021

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Rapamycin is an antifungal drug with antitumor activity and acts inhibiting the mTOR complex. Due to drug antitumor potential, the aim of this study was to evaluate its effect on a preclinical model of primary mammary gland tumors and their metastases from female dogs. Four cell lines from our cell bank, two from primary canine mammary tumors (UNESP-CM1, UNESP-CM60) and two metastases (UNESP-MM1, and UNESP-MM4) were cultured in vitro and investigated for rapamycin IC50. Then, cell lines were treated with rapamycin IC50 dose and mRNA and protein were extracted in treated and non-treated cells to perform AKT, mTOR, PTEN and 4EBP1 gene expression and global proteomics by mass spectrometry. MTT assay demonstrated rapamycin IC50 dose for all different tumor cells between 2 and 10 μM. RT-qPCR from cultured cells, control versus treated group and primary tumor cells versus metastatic tumor cells, did not shown statistical differences. In proteomics were found 273 proteins in all groups, and after data normalization 49 and 92 proteins were used for statistical analysis for comparisons between control versus rapamycin treatment groups, and metastasis versus primary tumor versus metastasis rapamycin versus primary tumor rapamycin, respectively. Considering the two statistical analysis, four proteins, phosphoglycerate mutase, malate dehydrogenase, l-lactate dehydrogenase and nucleolin were found in decreased abundance in the rapamycin group and they are related with cellular metabolic processes and enhanced tumor malignant behavior. Two proteins, dihydrolipoamide dehydrogenase and superoxide dismutase, also related with metabolic processes, were found in higher abundance in rapamycin group and are associated with apoptosis. The results suggested that rapamycin was able to inhibit cell growth of mammary gland tumor and metastatic tumors cells in vitro, however, concentrations needed to reach the IC50 were higher when compared to other studies.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 52%

Proteomics Approach of Rapamycin Anti-Tumoral Effect on Primary and Metastatic Canine Mammary Tumor Cells In Vitro

et al. 2021

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“…In addition to EMT mediators, DUBs target other molecules involved in cancer invasiveness. High expression of 14-3-3γ is found in various cancers, such as breast cancer and NSCLC [137,138]. Overexpression of 14-3-3γ promotes cell migration and invasion and correlates with the invasiveness of cancer cells.…”

Section: Dubs and Metastasismentioning

confidence: 99%

Role of Deubiquitinases in Human Cancers: Potential Targeted Therapy

Lai

Chen

Tse

2020

IJMS

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Deubiquitinases (DUBs) are involved in various cellular functions. They deconjugate ubiquitin (UBQ) from ubiquitylated substrates to regulate their activity and stability. Studies on the roles of deubiquitylation have been conducted in various cancers to identify the carcinogenic roles of DUBs. In this review, we evaluate the biological roles of DUBs in cancer, including proliferation, cell cycle control, apoptosis, the DNA damage response, tumor suppression, oncogenesis, and metastasis. This review mainly focuses on the regulation of different downstream effectors and pathways via biochemical regulation and posttranslational modifications. We summarize the relationship between DUBs and human cancers and discuss the potential of DUBs as therapeutic targets for cancer treatment. This review also provides basic knowledge of DUBs in the development of cancers and highlights the importance of DUBs in cancer biology.

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TXNDC9 knockdown inhibits lung adenocarcinoma progression by targeting YWHAG

Wang

Pan

et al. 2022

Mol Med Rep

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lung adenocarcinoma (luad) is the most common form of lung cancer and with the highest mortality rate. Therefore, the identification and development of effective methods for the treatment of luad is of great importance. The present study aimed to investigate the role of thioredoxin domain-containing protein 9 (TXndc9) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein γ (YWHaG; also known as 14-3-3γ) in the progression of luad. The expression of TXndc9 and its association with the survival of patients with luad was analyzed using encyclopedia of rna interactomes. reverse transcription-quantitative Pcr and western blot analysis were used to detect TXndc9 mrna and protein expression levels, respectively, in in vitro studies. To investigate the role of TXndc9 in the progression of luad, TXndc9 was silenced using small interfering rna transfection. Furthermore, the viability, proliferation, migration, invasiveness and apoptosis of TXndc9-silenced a549 cells were detected using cell counting Kit (ccK)-8, colony formation, wound healing, Transwell and Tunel assays, respectively. The association between TXndc9 and YWHaG was analyzed using STrinG and Gene Expression Profiling Interactive Analysis databases, as well as co-immunoprecipitation assays. Subsequently, YWHaG was overexpressed to similarly determine effects of YWHaG on viability, proliferation, migration, invasiveness and apoptosis of a549 cells. TXndc9 expression was markedly upregulated in lung cancer cells, particularly a549 cells, and silencing of TXndc9 expression suppressed the viability of the lung cancer cells. The results also revealed that TXndc9 silencing exerted inhibitory effects on the viability, proliferation, migration and invasiveness of a549 cells, whereas the apoptotic rate was increased. Similar to TXndc9, YWHaG expression was also upregulated in the a549 cells. Furthermore, TXndc9 was demonstrated to bind to YWHaG and was positively associated with YWHaG. YWHaG overexpression reversed the inhibitory effects of TXndc9 silencing on luad, as evidenced by increased viability, proliferation, migration and invasiveness, and decreased apoptosis, of a549 cells. The present study demonstrated that the knockdown of TXndc9 exerted suppressive effects on luad, whereas YWHaG overexpression reversed the inhibitory effects of TXndc9 silencing on luad. Therefore, TXndc9 silencing may exert protective effects against luad by targeting YWHaG.

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Correction to: Breast cancer cell motility is promoted by 14-3-3γ

Abstract: The correct name of the corresponding author should be ''Takahiro Mimae'', and not ''Emiko Hiraoka'' as given in the original publication of the article.

Cited by 3 publications

References 0 publications

Proteomics Approach of Rapamycin Anti-Tumoral Effect on Primary and Metastatic Canine Mammary Tumor Cells In Vitro

Proteomics Approach of Rapamycin Anti-Tumoral Effect on Primary and Metastatic Canine Mammary Tumor Cells In Vitro

Role of Deubiquitinases in Human Cancers: Potential Targeted Therapy

TXNDC9 knockdown inhibits lung adenocarcinoma progression by targeting YWHAG

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