Correlation Among the Expression, Clinical Significance and Prognosis of DJ-1 and Runt-Related Transcription Factor 3 in Rectal Cancer

Abstract

This study explored the correlation among the expression, clinical significance, and prognosis of DJ-1 and RUNX3 in rectal cancer. Eighty-five cases of resected rectal cancer tissue were obtained from patients treated from June 2014 to June 2015. The expression ofDJ-1 and RUNX3 mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR), and the expression of DJ-1 and RUNX3 protein was detected by immunohistochemistry. Their relationship to the clinicopathological features of the tumor was also analyzed. The correlation between the two proteins and survival prognosis [5-year disease-free survival(DFS)] was analyzed. RT-PCR results showed that the DJ-1 mRNA expression of cancer tissue increased, whereas that of RUNX3 decreased. Immunohistochemical results showed that the positive rate of DJ-1 protein of cancer tissue increased (69.41% vs. 21.18%), whereas that of RUNX3 decreased (40.00% vs. 77.65%). The positive expression of DJ-1 and RUNX3 was highly related to the cTNM staging of rectal cancer. Correlation analysis results showed a negative correlation between the genes in rectal cancer tissue. The follow-up records showed 5-year DFS data as follows: 50.00% for the DJ-1+/RUNX3− group, 57.14% for the DJ-1−/RUNX3− group, 71.43% for the DJ-1+/RUNX3+ group, and 82.50% for the DJ-1−/RUNX3+ group, suggesting that the 5-year DFS of the DJ-1+ group was lower, whereas that of the RUNX3+ group was higher. These results indicated that DJ-1 and RUNX3 were related to the occurrence, development, and progression of rectal cancer. Interestingly, there was a significant negative correlation between the genes, which was important for the treatment and prognosis of rectal cancer.