Correlation-based approaches to time-resolved fluorimetry

Hieftje, Gary M.; Haugen, G. R.

doi:10.1021/ac00229a002

Cited by 4 publications

(1 citation statement)

References 10 publications

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“…By multiplying the two time-varying wave forms together and integrating over all time, the cross-correlation of the waves is calculated. This is given by where τ is the delay time between the two functions and C 1,2 is the cross-correlation of the two functions f 1 and f 2 …”

Section: Resultsmentioning

confidence: 99%

Two-Channel Sequential Single-Molecule Measurement

et al. 1997

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Experimental results are given which demonstrate the sequential detection of single molecules with a measurement efficiency of near unity. IR140 dye molecules are detected in sequential probe volumes within a flowing stream through a 9 μm i.d. capillary. The measurement of single molecules was confirmed by means of autocorrelation, photobleaching, visual observation, and cross-correlation analysis. The number of single molecules photobleached prior to being measured in the second probe region was in excellent agreement with the bulk studies described by a photodestruction curve. A cross-correlation peak with a temporal delay corresponding to the interprobe volume transit time and a width in agreement with parabolic flow give a clear indication of sequential detection. The near unity measurement efficiencies for both channels indicates the great potential for rapid quantitative analysis of dilute solutions.

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Section: Resultsmentioning

confidence: 99%