Correlation between activity of ribonucleases and potato virus Y biosynthesis in tobacco plants

Antioxidant protection during ageing and senescence in chloroplasts of tobacco with modulated life span

“…Organelles were separated by the modified method of Š indelářová et al 34 Fresh leaves without the main veins were cut into pieces and homogenized in a homogenizer (Eta 1-012, Czechoslovakia) three times for 5 s with an ice-cold isolation medium (1:10 w (fresh mass, FM)/v) containing (pH 6.5) 10 mM sodium phosphate, 0.33 M sorbitol and 0.5% bovine serum albumin (BSA). The homogenates were filtered through two layers of cheesecloth and centrifuged at 2200g for 2 min at 48C.…”

Dark and light green tissues of tobacco leaves systemically infected with tobacco mosaic virus

“…So, ribonucleases (RNAses) seem to participate in plant defense mechanisms against viruses. Exogenous application of RNAses was reported to inhibit the development of viral pathogens, and RNAses were activated in infected plants (Diener 1961, Park et al 2004, Šindelářová and Šindelář 2004, Šindelářová et al 2000. The accumulation of barley stripe mosaic virus was significantly reduced in transgenic plants expressing a bacterial RNAse (Zhang et al 2001).…”

Ribonuclease activity of buckwheat

“…Šindelař and Šindelařova have observed positive correlation between RNase activi ty in tobacco and PVY reproduction as well [28]. The work of Šindelařova et al suggested that RNase activity is positively correlated with the susceptibility of tobacco cultivars to PVY [22]. This fact is obvious for susceptible cultivars.…”

“…BBV has negative-sense RNA genome and, taking into consideration its structural organization, may be included into Rhabdoviridae family [19]. So another motivation for involving BBV was that mechanism of rhabdovirus reproduction differs from that of positive-sense RNA genome virusestobacco mosaic virus, potato virus Y, bean pod mottle virus studied earlier in a similar sphere [8,[20][21][22].…”