Correlation between adult pyrethroid resistance and knockdown resistance (kdr) mutations in Aedes albopictus (Diptera: Culicidae) field populations in China

Abstract

BackgroundArboviral disease transmitted by Aedes albopictus such as dengue fever is an important threat to human health. Pyrethroid resistance raises a great challenge for mosquito control. A systematic assessment of Ae. albopictus resistance status in China is urgently needed, and the study of correlation between pyrethroid resistance and knockdown resistance (kdr) mutations would provide information to guide the control of the Ae. albopictus vector.MethodsFive field populations of Ae. albopictus were collected from Jinan (JN), Hangzhou (HZ), Baoshan (BS), Yangpu (YP) and Haikou (HK), China in 2017. Insecticide-impregnated papers were prepared with four pyrethroid chemicals, deltamethrin, permethrin, beta-cypermethrin and lambda-cyhalothrin. The susceptibility of Ae. albopictus to pyrethroids was tested by the WHO tube assay. Kdr mutations were identified by PCR and sequencing. Moreover, the correlation analysis between kdr alleles and pyrethroid resistance was performed.ResultsAll five populations of Ae. albopictus showed resistance to four pyrethroid insecticides. One kdr mutant allele at codon 1532 and three at 1534 were detected with frequency of 5.33% (I1532T), 44.20% (F1534S), 1.83% (F1534 L) and 0.87% (F1534C), respectively. Both 1532 and 1534 mutation mosquitoes were found in the BS and YP populations. Allele I1532T was negatively correlated with deltamethrin resistance phenotype (OR < 1), while F1534S mutation was positively correlated with deltamethrin and permethrin resistance (OR > 1).ConclusionsThe five field populations of Ae. albopictus adults were all resistant to deltamethrin, permethrin, beta-cypermethrin and lambda-cyhalothrin. Mutant F1534S was clearly associated with pyrethroid resistance phenotype in Ae. albopictus and this could be developed as a molecular marker to monitor the pyrethroid resistance problem in China.Electronic supplementary materialThe online version of this article (10.1186/s40249-018-0471-y) contains supplementary material, which is available to authorized users.

Bionomics and insecticide resistance of Aedes albopictus in Shandong, a high latitude and high-risk dengue transmission area in China

“…However, the other results showed differences. In the present study, the I1532T mutation, which appeared only in the mosquito populations from Italy and Hainan Province, China, was detected [45], and a previous study in Hainan showed that this mutation was negatively associated with the deltamethrin resistance phenotype [42]. However, in the present study, the I1532T mutation was positively correlated with the deltamethrin resistance phenotype.…”

“…Previous studies [29,[42][43][44] only detected non-synonymous mutations in domain III (1532 and 1534) in Ae. albopictus from China.…”

Phenotypic and molecular analysis of insecticide resistance in Aedes albopictus populations from Greece

“…Previous studies also reported relatively high mutation frequencies (reaching 66%) in populations sampled from the Attica region [47,48]. To date, kdr F1534C has also been recorded in Brazil [49], Singapore, Vietnam [50] and China [51], while other substitutions in the same genomic locus, F1534L and F1534S, also associated with pyrethroid resistance, have been identified in the USA, China, Vietnam and Italy [47,[50][51][52].…”

“…This mutation has been also monitored and reported at considerable frequencies in Ae. albopictus populations from Italy [47,48], Albania [48] and China [51]. Although Kasai et al, 2019 [50] demonstrated a lack of association between this mutation and resistance to pyrethroids, a recent study revealed that VGSC channels carrying this mutation have reduced sensitivity to type I pyrethoids, notably at similar levels to channels harbouring the mutation F1534S [23].…”

Bioassay and molecular monitoring of insecticide resistance status in Aedes albopictus populations from Greece, to support evidence-based vector control

“…This mutation has also been reported at considerable frequencies in Ae. albopictus populations from Italy [48,49], Albania [49] and China [52]. Although Kasai et al [51] demonstrated a lack of association between this mutation and resistance to pyrethroids, a recent study revealed that VGSC channels carrying this mutation have reduced sensitivity to type I pyrethoids, notably at similar levels to channels harbouring the F1534S mutation [24].…”